PMID- 33740692
OWN - NLM
STAT- MEDLINE
DCOM- 20210628
LR  - 20210628
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1171
DP  - 2021 May 1
TI  - Sensitive determination of methotrexate in plasma of children with acute leukemia 
      using double-solvent supramolecular systemas a novel extractant for dispersive 
      liquid-liquid microextraction.
PG  - 122628
LID - S1570-0232(21)00108-2 [pii]
LID - 10.1016/j.jchromb.2021.122628 [doi]
AB  - Methotrexate, as a folate antagonist, is one of the first anti-neoplasm drugs 
      offered and is still used as an effective drug in the treatment of various 
      malignancies. Methotrexate has a narrow treatment index and is associated with 
      numerous side effects.In thisresearch, for the first time a double-solvent 
      supramolecular system (DSS) was developed as an extractant without disperser 
      solvent for dispersive liquid-liquid microextraction (DLLME). DSS - DLLME was 
      applied to the extraction of methotrexate in plasma of children with acute 
      leukemiaprior to itsdetermination by high-performance liquid 
      chromatography-ultraviolet detection (HPLC - UV). In the present method, two long 
      normal chain alcohols are mixed in a particular ratio, and then it is injected 
      into the sample solution, which is on the magnetic stirrer. In this case, the 
      mixture of the two alcohol changes to new supramolecular aggregate. This new 
      supermolecule is used as an extractant, which has a higher extraction power than 
      any of its components alone. Under the optimum conditions, the calibration graph 
      was linear in the rage of 0.1-150 microg L(-1) with detection limit of 0.03 microg L(-1). 
      Relative standard deviations (RSDs) including intra-day and inter-day of method 
      based on7 replicate determinations of 100.0 microg L(-1)of methotrexate were 2.6% and 
      4.8%,respectively. The results proved that DSS - DLLME is a sensitive, very 
      simple, inexpensive, environmental friendly, rapid and efficient method for the 
      preconcentration of trace amount of drugs in biological samples.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Akramipour, Reza
AU  - Akramipour R
AD  - School of Medical, Kermanshah University of Medical Sciences, Kermanshah, Iran; 
      Clinical Research Development Center, Imam Khomeini and Mohammad Kermanshahi and 
      Farabi Hospitals, Kermanshah University of Medical Sciences, Kermanshah, Iran.
FAU - Fattahi, Nazir
AU  - Fattahi N
AD  - Research Center for Environmental Determinants of Health (RCEDH), Health 
      Inestitue, Kermanshah University of Medical Sciences, Kermanshah, Iran. 
      Electronic address: n.fattahi@kums.ac.ir.
FAU - Golpayegani, Mohammad Reza
AU  - Golpayegani MR
AD  - School of Medical, Kermanshah University of Medical Sciences, Kermanshah, Iran; 
      Clinical Research Development Center, Imam Khomeini and Mohammad Kermanshahi and 
      Farabi Hospitals, Kermanshah University of Medical Sciences, Kermanshah, Iran. 
      Electronic address: mrgolpayegani@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20210306
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Solvents)
RN  - YL5FZ2Y5U1 (Methotrexate)
SB  - IM
MH  - Acute Disease
MH  - Child
MH  - Chromatography, High Pressure Liquid/methods
MH  - Humans
MH  - Leukemia/*drug therapy
MH  - Limit of Detection
MH  - Linear Models
MH  - Liquid Phase Microextraction/*methods
MH  - Methotrexate/*blood/isolation & purification/therapeutic use
MH  - Reproducibility of Results
MH  - Solvents/chemistry
OTO - NOTNLM
OT  - Children with acute leukemia
OT  - Dispersive liquid-liquid microextraction
OT  - Double-solvent supramolecular system
OT  - Methotrexate
OT  - Plasma analysis
EDAT- 2021/03/20 06:00
MHDA- 2021/06/29 06:00
CRDT- 2021/03/19 20:23
PHST- 2020/08/28 00:00 [received]
PHST- 2021/02/20 00:00 [revised]
PHST- 2021/02/23 00:00 [accepted]
PHST- 2021/03/20 06:00 [pubmed]
PHST- 2021/06/29 06:00 [medline]
PHST- 2021/03/19 20:23 [entrez]
AID - S1570-0232(21)00108-2 [pii]
AID - 10.1016/j.jchromb.2021.122628 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2021 May 1;1171:122628. doi: 
      10.1016/j.jchromb.2021.122628. Epub 2021 Mar 6.