PMID- 33743514
OWN - NLM
STAT- MEDLINE
DCOM- 20210628
LR  - 20210628
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1171
DP  - 2021 May 1
TI  - Ultrasound-assisted solid phase microextraction-HPLC method based on 
      Fe(3)O(4)@SiO(2)-NH(2)-molecularly imprinted polymer magnetic nano-sorbent for 
      rapid and efficient extraction of harmaline from Peganum harmala extract.
PG  - 122640
LID - S1570-0232(21)00120-3 [pii]
LID - 10.1016/j.jchromb.2021.122640 [doi]
AB  - In the present study, a magnetic molecularly imprinted polymer (MMIP) was 
      synthesized for the extraction of harmaline from Peganum harmala by dispersive 
      solid-phase microextraction (DSPME). The MMIP for selective and intelligent 
      extraction of harmaline with excellent functionality and high selectivity was 
      synthesized using the sol-gel method with functionalized superparamagnetic 
      core-shell nanoparticles, ethylene glycol dimethacrylate (EDMA) as a 
      cross-linker, methacrylic acid (MAA) as a functional monomer, and 
      2,2-azobisisobutyronitrile (AIBN) as a porogen. To study the properties and 
      morphology of the coated polymer, FT-IR spectroscopy, FESEM, TEM images, and VSM 
      were used. The DSPME-HPLC-UV equipment was used to quantify and analyze the data 
      obtained from harmaline extraction. In this research, the efficiency of the 
      synthesized polymer in harmaline extraction was modeled and optimized using the 
      response surface methodology based on central composite design (RSM-CCD). In 
      addition, for modeling the isotherm of harmaline sorption by the MMIP, Langmuir 
      and Freundlich isotherm equations were used. The obtained results showed that the 
      extraction of harmaline with the MMIP was well described with Freundlich 
      isotherm. The results of the validation of the method showed that the measurement 
      of harmaline in the concentration range of 1.0-4000 ng mL(-1) followed a linear 
      relationship (R(2) = 9986.0). Moreover, the accuracy or repeatability index (% 
      RSD) was determined to be < 10, and the LOQ and LOD values were 0.526 and 
      0.158 ng mL(-1), respectively. The results of this study showed that the DSPME 
      technique by using the synthesized MMIP as an effective sorbent with high 
      efficiency and capacity could be utilized for pre-concentration and extraction of 
      harmaline from real and complex samples.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Sadegh, Negar
AU  - Sadegh N
AD  - Department of Chemistry, Faculty of Sciences, Shahrekord University, P.O. Box 
      115, Shahrekord, Iran.
FAU - Asfaram, Arash
AU  - Asfaram A
AD  - Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, 
      Iran. Electronic address: arash.asfaram@yums.ac.ir.
FAU - Javadian, Hamedreza
AU  - Javadian H
AD  - Department of Chemical Engineering, ETSEIB, Universitat Politecnica de Catalunya, 
      Diagonal 647, 08028 Barcelona, Spain.
FAU - Haddadi, Hedayat
AU  - Haddadi H
AD  - Department of Chemistry, Faculty of Sciences, Shahrekord University, P.O. Box 
      115, Shahrekord, Iran.
FAU - Sharifpour, Ebrahim
AU  - Sharifpour E
AD  - Social Determinants of Health Research Center, Yasuj University of Medical 
      Sciences, Yasuj, Iran.
LA  - eng
PT  - Journal Article
DEP - 20210309
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Molecularly Imprinted Polymers)
RN  - 0 (Plant Extracts)
RN  - CN58I4TOET (Harmaline)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - *Harmaline/analysis/chemistry/isolation & purification
MH  - Limit of Detection
MH  - Linear Models
MH  - Magnetite Nanoparticles/*chemistry
MH  - Molecular Imprinting/methods
MH  - Molecularly Imprinted Polymers/*chemistry
MH  - Peganum/*chemistry
MH  - Plant Extracts/chemistry
MH  - Reproducibility of Results
MH  - Solid Phase Microextraction/methods
MH  - Sonication/methods
OTO - NOTNLM
OT  - DSPME-HPLC-UV
OT  - Harmaline
OT  - Magnetic molecularly imprinted polymer (MMIP)
OT  - Solid-phase microextraction
EDAT- 2021/03/21 06:00
MHDA- 2021/06/29 06:00
CRDT- 2021/03/20 20:20
PHST- 2021/01/24 00:00 [received]
PHST- 2021/02/23 00:00 [revised]
PHST- 2021/02/27 00:00 [accepted]
PHST- 2021/03/21 06:00 [pubmed]
PHST- 2021/06/29 06:00 [medline]
PHST- 2021/03/20 20:20 [entrez]
AID - S1570-0232(21)00120-3 [pii]
AID - 10.1016/j.jchromb.2021.122640 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2021 May 1;1171:122640. doi: 
      10.1016/j.jchromb.2021.122640. Epub 2021 Mar 9.