PMID- 33746885
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210323
IS  - 1664-2295 (Print)
IS  - 1664-2295 (Electronic)
IS  - 1664-2295 (Linking)
VI  - 12
DP  - 2021
TI  - Adiponectin-Transfected Endothelial Progenitor Cells Have Protective Effects 
      After 2-Hour Middle-Cerebral Artery Occlusion in Rats With Type 2 Diabetes 
      Mellitus.
PG  - 630681
LID - 10.3389/fneur.2021.630681 [doi]
LID - 630681
AB  - Objectives: This present study aimed to examine the effects of 
      adiponectin-transfected endothelial progenitor cells (LV-APN-EPCs) on cerebral 
      ischemia-reperfusion injury in rats with type 2 diabetes mellitus (T2DM) and to 
      explore the underlying mechanisms. Methods: Seventy male Sprague-Dawley rats with 
      T2DM were randomly divided into sham, phosphate-buffered saline (PBS), 
      LV-APN-EPCs, LV-EPCs, and EPCs groups. Transient middle cerebral artery occlusion 
      (MCAO) was induced by the intraluminal suture method. After 1 h of reperfusion, 
      the five interventions were performed by tail-vein injections. The modified 
      neurological severity score (mNSS) was used to assess neurological function 
      before and on days 1, 7, and 14 after MCAO. After 14 days, magnetic resonance 
      imaging scanning, hematoxylin and eosin staining, terminal dUTP nick-end labeling 
      staining, Western blotting analysis, cluster of differentiation (CD) 31 
      immunofluorescence, and enzyme-linked immunosorbent assay were used to evaluate 
      infarct rate, morphological damage, cell apoptosis, and microvessel density. 
      Results: Compared with PBS, LV-EPCs, and EPCs groups, the LV-APN-EPCs group 
      showed significantly lower mNSS score, lower infarct rate, and less morphological 
      damage (all P < 0.05). In addition, compared with other groups, the LV-APN-EPCs 
      group had significantly increased levels of B cell lymphoma/leukemia-2 (Bcl-2) 
      protein, CD31+ microvessels, endothelial nitric oxide synthase, and vascular 
      endothelial growth factor, and decreased levels of Bcl-2-associated X protein and 
      neuronal apoptosis in the peri-infarct cortex (all P < 0.05). Conclusion: These 
      results suggest that LV-APN-EPCs exert protective effects against cerebral 
      ischemia-reperfusion injury in T2DM rats by increasing angiogenesis.
CI  - Copyright (c) 2021 Wang, Li, Zhang, Zhang, Feng, Kong, Aiziretiaili, Luo, Cai, Hong 
      and Liu.
FAU - Wang, Meiyao
AU  - Wang M
AD  - Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, China.
AD  - Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, China.
FAU - Li, Yan
AU  - Li Y
AD  - Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, China.
FAU - Zhang, Renwei
AU  - Zhang R
AD  - Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, China.
FAU - Zhang, Shuaimei
AU  - Zhang S
AD  - Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, China.
FAU - Feng, Hongliang
AU  - Feng H
AD  - Guangdong Mental Health Center, Guangdong Provincial People's Hospital, Guangdong 
      Academy of Medical Sciences, Guangzhou, China.
FAU - Kong, Zhaohong
AU  - Kong Z
AD  - Department of Neurology, Renmin Hospital of Wuhan University, Wuhan, China.
FAU - Aiziretiaili, Nadire
AU  - Aiziretiaili N
AD  - Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, China.
FAU - Luo, Zhengjin
AU  - Luo Z
AD  - Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, China.
FAU - Cai, Qi
AU  - Cai Q
AD  - Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, China.
FAU - Hong, Yan
AU  - Hong Y
AD  - Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, China.
FAU - Liu, Yumin
AU  - Liu Y
AD  - Department of Neurology, Zhongnan Hospital of Wuhan University, Wuhan, China.
LA  - eng
PT  - Journal Article
DEP - 20210303
PL  - Switzerland
TA  - Front Neurol
JT  - Frontiers in neurology
JID - 101546899
PMC - PMC7966523
OTO - NOTNLM
OT  - acute ischemic stroke
OT  - adiponectin-transfected endothelial progenitor cell
OT  - angiogenesis
OT  - protective effects
OT  - type 2 diabetes mellitus
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/03/23 06:00
MHDA- 2021/03/23 06:01
PMCR- 2021/03/03
CRDT- 2021/03/22 08:08
PHST- 2020/11/18 00:00 [received]
PHST- 2021/01/28 00:00 [accepted]
PHST- 2021/03/22 08:08 [entrez]
PHST- 2021/03/23 06:00 [pubmed]
PHST- 2021/03/23 06:01 [medline]
PHST- 2021/03/03 00:00 [pmc-release]
AID - 10.3389/fneur.2021.630681 [doi]
PST - epublish
SO  - Front Neurol. 2021 Mar 3;12:630681. doi: 10.3389/fneur.2021.630681. eCollection 
      2021.