PMID- 33748159
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210323
IS  - 2296-858X (Print)
IS  - 2296-858X (Electronic)
IS  - 2296-858X (Linking)
VI  - 8
DP  - 2021
TI  - ZBTB20 in Nociceptive Neurons of the Trigeminal Ganglia Regulates Pruritus.
PG  - 626554
LID - 10.3389/fmed.2021.626554 [doi]
LID - 626554
AB  - Recent studies have shown that ZBTB20, a zinc-finger protein containing 
      transcription factor, is highly expressed in small-diameter primary sensory 
      neurons in mice, and modulates pain through regulating TRP channels. However, 
      whether ZBTB20 regulates itch sensation has not been demonstrated. In this study, 
      small-diameter primary sensory neuron-specific ZBTB20 knockout (PN-ZB20KO) mice 
      were used to investigate the role of ZBTB20 in the regulation of itch sensation. 
      First, both histamine-dependent and non-histamine-dependent itch behaviors 
      induced by injection of histamine and chloroquine (CQ) into the cheek were 
      significantly diminished in PN-ZB20KO mice. Second, double immunohistochemistry 
      showed that ZBTB20 was mainly expressed in CGRP-labeled small peptidergic neurons 
      and was expressed at low levels in IB4-labeled small non-peptidergic and 
      NF200-labeled large neurons in the trigeminal ganglia (TG). ZBTB20 was also 
      expressed in most TRPV1(+) and TRPA1(+) neurons and to a lesser extent in 
      TRPM8(+) neurons in the TG. Furthermore, cheek injection of histamine and CQ 
      enhanced the mRNA expression of TRPV1 and TRPA1 but not TRPM8 in the TG. 
      Moreover, TRPV1 and TRPA1 knockout (KO) mice exhibited attenuation of itch 
      behavior induced by histamine and CQ, respectively. Finally, silencing endogenous 
      ZBTB20 with recombinant lentivirus expressing a short hairpin RNA against ZBTB20 
      (LV-shZBTB20) in TG neurons attenuated histamine- and non-histamine-induced itch 
      and downregulated TRP channels in the TG. Our study suggests that ZBTB20 plays an 
      important role in mediating itch in small primary sensory neurons.
CI  - Copyright (c) 2021 Jia, Dai, Ren, Wang, Zhang and Zhang.
FAU - Jia, Xin
AU  - Jia X
AD  - The First Rehabilitation Hospital of Shanghai, Tongji University School of 
      Medicine, Shanghai, China.
AD  - Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of 
      Ministry of Education, Orthopaedic Department of Tongji Hospital, School of 
      Medicine, Tongji University, Shanghai, China.
FAU - Dai, Meng-Han
AU  - Dai MH
AD  - The First Rehabilitation Hospital of Shanghai, Tongji University School of 
      Medicine, Shanghai, China.
AD  - Department of Anesthesiology, Renmin Hospital, Hubei University of Medicine, 
      Shiyan, China.
FAU - Ren, An-Jing
AU  - Ren AJ
AD  - Department of Pathophysiology, Naval Medical University, Shanghai, China.
FAU - Wang, Ting-Ting
AU  - Wang TT
AD  - The First Rehabilitation Hospital of Shanghai, Tongji University School of 
      Medicine, Shanghai, China.
AD  - Department of Dermatology, Tongren Hospital Shanghai Jiao Tong University School 
      of Medicine, Shanghai, China.
FAU - Zhang, Weiping J
AU  - Zhang WJ
AD  - Department of Pathophysiology, Naval Medical University, Shanghai, China.
AD  - NHC Key Laboratory of Hormones and Development, Tianjin Institute of 
      Endocrinology, Tianjin Medical University Chu Hsien-I Memorial Hospital, Tianjin, 
      China.
FAU - Zhang, Ling
AU  - Zhang L
AD  - The First Rehabilitation Hospital of Shanghai, Tongji University School of 
      Medicine, Shanghai, China.
AD  - Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of 
      Ministry of Education, Orthopaedic Department of Tongji Hospital, School of 
      Medicine, Tongji University, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20210304
PL  - Switzerland
TA  - Front Med (Lausanne)
JT  - Frontiers in medicine
JID - 101648047
PMC - PMC7969640
OTO - NOTNLM
OT  - TRPA1
OT  - TRPV1
OT  - ZBTB20
OT  - itch
OT  - pain
OT  - pruritus
OT  - trigeminal ganglia
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/03/23 06:00
MHDA- 2021/03/23 06:01
PMCR- 2021/03/04
CRDT- 2021/03/22 08:22
PHST- 2020/11/06 00:00 [received]
PHST- 2021/01/11 00:00 [accepted]
PHST- 2021/03/22 08:22 [entrez]
PHST- 2021/03/23 06:00 [pubmed]
PHST- 2021/03/23 06:01 [medline]
PHST- 2021/03/04 00:00 [pmc-release]
AID - 10.3389/fmed.2021.626554 [doi]
PST - epublish
SO  - Front Med (Lausanne). 2021 Mar 4;8:626554. doi: 10.3389/fmed.2021.626554. 
      eCollection 2021.