PMID- 33749119
OWN - NLM
STAT- MEDLINE
DCOM- 20220204
LR  - 20220204
IS  - 1755-148X (Electronic)
IS  - 1755-1471 (Linking)
VI  - 34
IP  - 5
DP  - 2021 Sep
TI  - The role of melanocytes in the human choroidal microenvironment and inflammation: 
      Insights from the transcriptome.
PG  - 928-945
LID - 10.1111/pcmr.12972 [doi]
AB  - The choroid within the human eye contains a rich milieu of cells including 
      melanocytes. Human choroidal melanocytes (HCMs) absorb light, regulate free 
      radical production, and were recently shown to modulate inflammation. This study 
      aimed to identify key genes and pathways involved in the inflammatory response of 
      HCMs through the use of RNA-seq. Primary HCMs were cultured from donor choroids, 
      RNA was extracted from control and lipopolysaccharide (LPS)-treated HCMs, and 
      mRNA was sequenced. Functional annotation and pathway analysis were performed 
      using gene ontology and gene set enrichment analyses. Representative RNA-seq 
      results were verified with RT-qPCR and protein measurements. We detected 100 
      differentially expressed genes including an array of CCL and CXCL cytokines and 
      mediators of cell-cell and cell-matrix adhesion, such as ICAM1, CLDN1, CCN3, 
      ITGA1 and ITGA11. Functional annotation showed that these gene sets control 
      inflammatory pathways, immune cell trafficking, cell-cell adhesion, interactions 
      with the extracellular matrix and blood vessels, angiogenesis and 
      epithelial-to-mesenchymal transitions. Our study provides insights into the 
      transcriptional regulation of primary HCMs in response to inflammatory stimuli 
      and identifies novel melanocyte-driven mechanisms potentially involved in 
      choroidal homeostasis and inflammation.
CI  - (c) 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Cioanca, Adrian V
AU  - Cioanca AV
AUID- ORCID: 0000-0003-1379-9993
AD  - John Curtin School of Medical Research, The Australian National University, 
      Canberra, ACT, Australia.
FAU - Wu, Chieh-Lin Stanley
AU  - Wu CS
AD  - School of Optometry and Vision Science, University of NSW, Sydney, NSW, 
      Australia.
AD  - Save Sight Institute, University of Sydney, Sydney, NSW, Australia.
FAU - Natoli, Riccardo
AU  - Natoli R
AD  - John Curtin School of Medical Research, The Australian National University, 
      Canberra, ACT, Australia.
FAU - Conway, R Max
AU  - Conway RM
AD  - Save Sight Institute, University of Sydney, Sydney, NSW, Australia.
FAU - McCluskey, Peter J
AU  - McCluskey PJ
AD  - Save Sight Institute, University of Sydney, Sydney, NSW, Australia.
FAU - Jager, Martine J
AU  - Jager MJ
AD  - Department of Ophthalmology, Leiden University Medical Center, Leiden, The 
      Netherlands.
FAU - Sitiwin, Ephrem I
AU  - Sitiwin EI
AD  - Save Sight Institute, University of Sydney, Sydney, NSW, Australia.
FAU - Eamegdool, Steven S
AU  - Eamegdool SS
AD  - Save Sight Institute, University of Sydney, Sydney, NSW, Australia.
AD  - Eye Genetics Research Unit, Children's Medical Research Institute, Westmead 
      Hospital, Westmead, NSW, Australia.
FAU - Madigan, Michele C
AU  - Madigan MC
AD  - School of Optometry and Vision Science, University of NSW, Sydney, NSW, 
      Australia.
AD  - Save Sight Institute, University of Sydney, Sydney, NSW, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210331
PL  - England
TA  - Pigment Cell Melanoma Res
JT  - Pigment cell & melanoma research
JID - 101318927
SB  - IM
MH  - *Cellular Microenvironment
MH  - Choroid/cytology/*metabolism
MH  - Humans
MH  - Melanocytes/cytology/*metabolism
MH  - *RNA-Seq
MH  - *Transcriptome
OTO - NOTNLM
OT  - adhesion
OT  - choroid
OT  - extracellular matrix
OT  - inflammation
OT  - melanocortin receptor 1
OT  - melanocytes
OT  - vasculature
EDAT- 2021/03/23 06:00
MHDA- 2022/02/05 06:00
CRDT- 2021/03/22 08:49
PHST- 2021/02/22 00:00 [revised]
PHST- 2020/11/17 00:00 [received]
PHST- 2021/03/13 00:00 [accepted]
PHST- 2021/03/23 06:00 [pubmed]
PHST- 2022/02/05 06:00 [medline]
PHST- 2021/03/22 08:49 [entrez]
AID - 10.1111/pcmr.12972 [doi]
PST - ppublish
SO  - Pigment Cell Melanoma Res. 2021 Sep;34(5):928-945. doi: 10.1111/pcmr.12972. Epub 
      2021 Mar 31.