PMID- 33749571
OWN - NLM
STAT- MEDLINE
DCOM- 20220413
LR  - 20220413
IS  - 1475-2662 (Electronic)
IS  - 0007-1145 (Linking)
VI  - 127
IP  - 3
DP  - 2022 Feb 14
TI  - Interactions between dietary carbohydrate and thiamine: implications on the 
      growth performance and intestinal mitochondrial biogenesis and function of 
      Megalobrama amblycephala.
PG  - 321-334
LID - 10.1017/S000711452100101X [doi]
AB  - A12-week experiment was conducted to evaluate the influences of thiamine ongrowth 
      performance, and intestinal mitochondrial biogenesis and function 
      of Megalobramaamblycephala fed a high-carbohydrate (HC) diet. Fish (24.73 (sem 
      0.45) g) were randomly assigned to one of four diets: two carbohydrate (CHO) 
      levels (30 and 45 %) and two thiamine levels (0 and 1.5 mg/kg). HC diets 
      significantly decreased DGC, GRMBW, FIMBW, intestinal activities of amylase, 
      lipase, Na+, K+-ATPase, CK, complexes I, III and IV, intestinal ML, number of 
      mitochondrial per field, DeltaPsim, the P-AMPK: T-AMPK ratio, PGC-1beta protein expression 
      as well as the transcriptions of AMPKalpha1, AMPKalpha2, PGC-1beta, mitochondrial 
      transcription factor A, Opa-1, ND-1 and COX-1 and 2, while the opposite was true 
      for ATP, AMP and reactive oxygen species, and the transcriptions of 
      dynamin-related protein-1, fission-1 and mitochondrial fission factor. 
      Dietarythiamine concentrations significantly increased DGC, GRMBW, intestinal 
      activities of amylase, Na+, K+-ATPase, CK, complexes I and IV, intestinal ML, 
      number of mitochondrial per field, DeltaPsim, the P-AMPK:T-AMPK ratio, PGC-1beta protein 
      expression as well as the transcriptions of AMPKalpha1, AMPKalpha2, PGC-1beta, Opa-1, ND-1, 
      COX-1 and 2, SGLT-1 and GLUT-2. Furthermore, a significant interaction between 
      dietary CHO and thiamine was observed in DGC, GRMBW, intestinal activities of 
      amylase, CK, complexes I and IV, DeltaPsim, the AMP:ATP ratio, the P-AMPK:T-AMPK ratio, 
      PGC-1beta protein expression as well as the transcriptions of AMPKalpha1, AMPKalpha2, 
      PGC-1beta, Opa-1, COX-1 and 2, SGLT-1 and GLUT-2. Overall, thiamine supplementation 
      improved growth performance, and intestinal mitochondrial biogenesis and function 
      of M. amblycephala fed HC diets.
FAU - Xu, Chao
AU  - Xu C
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou510642, 
      People's Republic of China.
FAU - Li, Yuan-You
AU  - Li YY
AUID- ORCID: 0000-0002-1058-2484
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou510642, 
      People's Republic of China.
FAU - Brown, Paul B
AU  - Brown PB
AD  - Purdue University, Department of Forestry and Natural Resources, West Lafayette, 
      IN, 47907, USA.
FAU - Liu, Wen-Bin
AU  - Liu WB
AD  - Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College 
      of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang 
      Road, Nanjing210095, People's Republic of China.
FAU - Gao, Liu-Ling
AU  - Gao LL
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou510642, 
      People's Republic of China.
FAU - Ding, Zhi-Rong
AU  - Ding ZR
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou510642, 
      People's Republic of China.
FAU - Li, Xiang-Fei
AU  - Li XF
AUID- ORCID: 0000-0003-0841-0584
AD  - Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College 
      of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang 
      Road, Nanjing210095, People's Republic of China.
FAU - Xie, Di-Zhi
AU  - Xie DZ
AD  - College of Marine Sciences, South China Agricultural University, Guangzhou510642, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210322
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 415SHH325A (Adenosine Monophosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - EC 3.2.1.- (Amylases)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - X66NSO3N35 (Thiamine)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Adenosine Monophosphate
MH  - Adenosine Triphosphatases/metabolism
MH  - Adenosine Triphosphate/metabolism
MH  - Amylases/metabolism
MH  - Animals
MH  - *Dietary Carbohydrates/metabolism/pharmacology
MH  - *Organelle Biogenesis
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha/metabolism
MH  - Thiamine/pharmacology
OTO - NOTNLM
OT  - Carbohydrate utilisation
OT  - Fish culture
OT  - Intestinal function
OT  - Mitochondrial biogenesis and function
OT  - Thiamine
EDAT- 2021/03/23 06:00
MHDA- 2022/04/14 06:00
CRDT- 2021/03/22 12:35
PHST- 2021/03/23 06:00 [pubmed]
PHST- 2022/04/14 06:00 [medline]
PHST- 2021/03/22 12:35 [entrez]
AID - S000711452100101X [pii]
AID - 10.1017/S000711452100101X [doi]
PST - ppublish
SO  - Br J Nutr. 2022 Feb 14;127(3):321-334. doi: 10.1017/S000711452100101X. Epub 2021 
      Mar 22.