PMID- 33750035
OWN - NLM
STAT- MEDLINE
DCOM- 20211101
LR  - 20220606
IS  - 2472-1727 (Electronic)
VI  - 113
IP  - 12
DP  - 2021 Jul 15
TI  - Verbascoside-enriched fraction from Buddleja cordata Kunth ameliorates the 
      effects of diabetic embryopathy in an animal model.
PG  - 981-994
LID - 10.1002/bdr2.1894 [doi]
AB  - BACKGROUND: The deleterious effects of diabetes mellitus (DM) over development 
      are apparently due to an increase in oxidative stress. Some antioxidants could 
      prevent developmental alterations produced by diabetic state. Extracts of plants 
      of the genus Buddleja are used traditionally for Mexican indigens to ameliorate 
      some diseases. The purpose of this work was to evaluate the effect of the extract 
      of Buddleja cordata over diabetic embryopathy. METHODS: Two experimental 
      approaches were used: an in vivo study and an in vitro model. In the first, rats 
      were treated with streptozotocin, streptozotocin plus methanolic extract of B. 
      cordata, or none. Females were sacrificed at gestational day (GD) 19, and 
      biochemical clinical parameters were measured; also, the fetuses were obtained 
      and morphologically analyzed. In the in vitro model, a verbascoside-enriched 
      fraction (VEF) of the extract was used in whole embryo culture in order to search 
      for the mechanisms for embryoprotection effect over hyperglycemia-induced 
      malformations. RESULTS: In the in vivo experiments, B. cordata extract reduces 
      the frequency and severity of fetal malformations produced by chemically induced 
      diabetes, and additionally partially ameliorates the diabetic condition; in the 
      in vitro model, both severity and frequency of embryo dysmorphogenesis were 
      reduced by the VEF; also, this fraction reduces lipoperoxidation without 
      affecting the activity of the antioxidant enzymes. CONCLUSION: The results 
      suggest that verbascoside of methanolic extract and enriched fraction can 
      directly affect the redox state, and thus, prevents the embryotoxicity mediated 
      by oxidative stress, in embryos of diabetic pregnancy.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Chirino-Galindo, Gladys
AU  - Chirino-Galindo G
AD  - Laboratorio de Metabolismo de la Diabetes Mellitus, Unidad de Morfofisiologia, 
      Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de 
      Mexico, Mexico, Mexico.
FAU - Lopez-Quintero, Ilse-Valeria
AU  - Lopez-Quintero IV
AD  - Laboratorio de Metabolismo de la Diabetes Mellitus, Unidad de Morfofisiologia, 
      Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de 
      Mexico, Mexico, Mexico.
FAU - Ramirez-Dominguez, Liliana-Berenice
AU  - Ramirez-Dominguez LB
AD  - Laboratorio de Metabolismo de la Diabetes Mellitus, Unidad de Morfofisiologia, 
      Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de 
      Mexico, Mexico, Mexico.
FAU - Cabrera-Najera, Leonardo-Elias
AU  - Cabrera-Najera LE
AD  - Laboratorio de Metabolismo de la Diabetes Mellitus, Unidad de Morfofisiologia, 
      Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de 
      Mexico, Mexico, Mexico.
FAU - Estrella-Parra, Edgar-Antonio
AU  - Estrella-Parra EA
AD  - Laboratorio de Fitoquimica, Unidad de Biologia y Prototipos, Facultad de Estudios 
      Superiores Iztacala, Universidad Nacional Autonoma de Mexico, Mexico, Mexico.
FAU - Garcia-Bores, Ana-Maria
AU  - Garcia-Bores AM
AD  - Laboratorio de Fitoquimica, Unidad de Biologia y Prototipos, Facultad de Estudios 
      Superiores Iztacala, Universidad Nacional Autonoma de Mexico, Mexico, Mexico.
FAU - Palomar-Morales, Martin
AU  - Palomar-Morales M
AUID- ORCID: 0000-0003-1382-276X
AD  - Laboratorio de Metabolismo de la Diabetes Mellitus, Unidad de Morfofisiologia, 
      Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de 
      Mexico, Mexico, Mexico.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210322
PL  - United States
TA  - Birth Defects Res
JT  - Birth defects research
JID - 101701004
RN  - 0 (Glucosides)
RN  - 0 (Phenols)
RN  - 3TGX09BD5B (acteoside)
SB  - IM
EIN - Birth Defects Res. 2022 Jul 15;114(12):683. PMID: 35666081
MH  - Animals
MH  - *Buddleja
MH  - *Diabetes Mellitus
MH  - Disease Models, Animal
MH  - *Fetal Diseases
MH  - Glucosides
MH  - Phenols
MH  - Pregnancy
MH  - Rats
OTO - NOTNLM
OT  - Buddleja cordata
OT  - embryo culture
OT  - embryoprotection
OT  - oxidative stress
OT  - teratogenesis
EDAT- 2021/03/23 06:00
MHDA- 2021/11/03 06:00
CRDT- 2021/03/22 13:05
PHST- 2021/02/24 00:00 [revised]
PHST- 2020/10/01 00:00 [received]
PHST- 2021/03/14 00:00 [accepted]
PHST- 2021/03/23 06:00 [pubmed]
PHST- 2021/11/03 06:00 [medline]
PHST- 2021/03/22 13:05 [entrez]
AID - 10.1002/bdr2.1894 [doi]
PST - ppublish
SO  - Birth Defects Res. 2021 Jul 15;113(12):981-994. doi: 10.1002/bdr2.1894. Epub 2021 
      Mar 22.