PMID- 33751905
OWN - NLM
STAT- MEDLINE
DCOM- 20210625
LR  - 20210625
IS  - 1557-7600 (Electronic)
IS  - 1096-620X (Linking)
VI  - 24
IP  - 6
DP  - 2021 Jun
TI  - Anthocyanin-Rich Aronia Berry Extract Mitigates High-Fat and High-Sucrose 
      Diet-Induced Adipose Tissue Inflammation by Inhibiting Nuclear Factor-kappaB 
      Activation.
PG  - 586-594
LID - 10.1089/jmf.2020.0127 [doi]
AB  - Obesity-induced inflammation in adipose tissue (AT) promotes the development of 
      metabolic dysregulations by increasing macrophage recruitment in the stromal 
      vascular fraction (SVF). The activation of nuclear factor-kappaB (NF-kappaB) signaling in 
      macrophages serves as a pivotal mediator of AT inflammatory responses by 
      increasing the expression of proinflammatory genes in obesity. Given the 
      purported anti-inflammatory effects of berry consumption in humans, we evaluated 
      if anthocyanin-rich aronia berry extract (ARN) can prevent obesity-induced AT 
      inflammation in vivo. We also examined whether ARN suppresses lipopolysaccharide 
      (LPS)-induced NF-kappaB activation in RAW 264.7 macrophages and mouse bone 
      marrow-derived macrophages (BMDMs). Male C57BL/6J mice were fed a low-fat diet, a 
      high-fat (HF), and high-sucrose (HS) diet or HF/HS diet supplemented with 0.2% 
      ARN (HF/HS + ARN) for 14 weeks. Compared to HF-/HS-fed mice, ARN supplementation 
      tended to decrease fasting serum glucose (P = .07). Furthermore, ARN 
      supplementation significantly inhibited the phosphorylation of NF-kappaB p65 in 
      epididymal AT with a concomitant decrease in the expression of Cd11b and Tnfalpha 
      mRNAs in epididymal SVF isolated, compared with those from HF-/HS-fed mice. 
      Consistent with these in vivo findings, ARN treatment significantly decreased the 
      phosphorylation of p65 in LPS-stimulated RAW 264.7 macrophages and BMDMs. 
      Moreover, ARN suppressed LPS-induced mRNA expression of inflammation mediators 
      (iNos, Cox-2, Tnfalpha, Mcp-1, and Il-6) and glycolysis markers (Glut1, G6pdh, and 
      Hk1) in both cell types. Taken together, our in vivo and in vitro results suggest 
      that ARN supplementation may attenuate obesity-induced AT inflammation by 
      inhibiting NF-kappaB signaling and glycolytic pathway in macrophages.
FAU - Yu, Seok-Yeong
AU  - Yu SY
AD  - Department of Nutrition, University of Massachusetts, Amherst, Massachusetts, 
      USA.
FAU - Kim, Mi-Bo
AU  - Kim MB
AD  - Department of Nutritional Sciences, University of Connecticut, Storrs, 
      Connecticut, USA.
FAU - Park, Young-Ki
AU  - Park YK
AD  - Department of Nutritional Sciences, University of Connecticut, Storrs, 
      Connecticut, USA.
FAU - Bae, Minkyung
AU  - Bae M
AD  - Department of Nutritional Sciences, University of Connecticut, Storrs, 
      Connecticut, USA.
FAU - Kang, Hyunju
AU  - Kang H
AD  - Department of Nutritional Sciences, University of Connecticut, Storrs, 
      Connecticut, USA.
FAU - Hu, Siqi
AU  - Hu S
AD  - Department of Nutritional Sciences, University of Connecticut, Storrs, 
      Connecticut, USA.
FAU - Pham, Tho X
AU  - Pham TX
AD  - Department of Nutritional Sciences, University of Connecticut, Storrs, 
      Connecticut, USA.
FAU - Carpenter, Ryan
AU  - Carpenter R
AD  - Department of Chemical Engineering, University of Massachusetts, Amherst, 
      Massachusetts, USA.
FAU - Lee, Jungwoo
AU  - Lee J
AD  - Department of Chemical Engineering, University of Massachusetts, Amherst, 
      Massachusetts, USA.
FAU - Lee, Ok-Hwan
AU  - Lee OH
AD  - Department of Food Science and Biotechnology, Kangwon National University, 
      Chuncheon, Korea.
FAU - Lee, Ji-Young
AU  - Lee JY
AD  - Department of Nutritional Sciences, University of Connecticut, Storrs, 
      Connecticut, USA.
FAU - Kim, Young-Cheul
AU  - Kim YC
AD  - Department of Nutrition, University of Massachusetts, Amherst, Massachusetts, 
      USA.
LA  - eng
PT  - Journal Article
DEP - 20210322
PL  - United States
TA  - J Med Food
JT  - Journal of medicinal food
JID - 9812512
RN  - 0 (Anthocyanins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - 57-50-1 (Sucrose)
SB  - IM
MH  - Adipose Tissue
MH  - Animals
MH  - Anthocyanins
MH  - Diet, High-Fat/adverse effects
MH  - Inflammation/drug therapy/genetics
MH  - Lipopolysaccharides
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *NF-kappa B/genetics
MH  - *Photinia
MH  - Plant Extracts/pharmacology
MH  - Sucrose
OTO - NOTNLM
OT  - NF-kappaB signaling
OT  - adipose tissue inflammation
OT  - anthocyanin-rich aronia extract
OT  - bone marrow-derived macrophage
EDAT- 2021/03/23 06:00
MHDA- 2021/06/29 06:00
CRDT- 2021/03/22 20:10
PHST- 2021/03/23 06:00 [pubmed]
PHST- 2021/06/29 06:00 [medline]
PHST- 2021/03/22 20:10 [entrez]
AID - 10.1089/jmf.2020.0127 [doi]
PST - ppublish
SO  - J Med Food. 2021 Jun;24(6):586-594. doi: 10.1089/jmf.2020.0127. Epub 2021 Mar 22.