PMID- 33754017 OWN - NLM STAT- MEDLINE DCOM- 20210816 LR - 20210816 IS - 1838-7640 (Electronic) IS - 1838-7640 (Linking) VI - 11 IP - 10 DP - 2021 TI - Decreased neuronal synaptosome associated protein 29 contributes to poststroke cognitive impairment by disrupting presynaptic maintenance. PG - 4616-4636 LID - 10.7150/thno.54210 [doi] AB - Background: Poststroke cognitive impairments are common in stroke survivors, and pose a high risk of incident dementia. However, the cause of these cognitive impairments is obscure and required an investigation. Methods: Oxygen-glucose deprivation (OGD) model and middle cerebral artery occlusion (MCAO) model were used to imitate in vitro or in vivo acute cerebral ischemia, respectively. The differentially expressed synaptosome associated protein 29 (SNAP29)-interacting proteins upon ischemia and reperfusion were analyzed with bioinformatics analysis and the results indicated that the changes of SNAP29 after acute ischemia were mainly involved in the synaptic functions. The outcomes of SNAP29 reduction were assessed with SNAP29 knockdown, which mimicked the distribution of SNAP29 along neuronal processes after acute ischemia. Using the whole-cell patch clamp recording method and transmission electron microscope, the pre-synaptic function and readily releasable pool (RRP) were observed after SNAP29 knock down. Using photogenetic manipulations and behavioral tests, the neuronal projection and cognitive functions of mice with SNAP29 knock down in hippocampus CA1 region were evaluated. Results: It was found that SNAP29 protein levels decreased in both in vitro and in vivo ischemic models. Further, the SNAP29 reduction wasn't associated with impaired autophagy flux and neuronal survival. When SNAP29 was knocked down in primary cortical neurons, the frequency of AMPARs-mediated mEPSCs, but not the amplitude, significantly decreased. Meanwhile, the mice with SNAP29 knockdown at CA1 region of hippocampus developed an impairment in hippocampus-mPFC (middle prefrontal cortex) circuit and behavioral dysfunctions. Moreover, the size of RRP at presynaptic sites was diminished. Conclusion: Since SNAP29 protein levels didn't significantly influence the neuronal survival and its decrease was sufficient to disturb the neural circuit via a presynaptic manner, the SNAP29-associated strategies may be an efficient target against poststroke synaptic dysfunction and cognitive deficits. CI - (c) The author(s). FAU - Yan, Weijie AU - Yan W AD - Department of Neurobiology, Department of Physiology and Pathophysiology, Key Laboratory for Neurodegenerative Disorders of the Ministry of Education, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China. FAU - Fan, Jiahui AU - Fan J AD - Department of Neurobiology, Department of Physiology and Pathophysiology, Key Laboratory for Neurodegenerative Disorders of the Ministry of Education, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China. FAU - Zhang, Xia AU - Zhang X AD - Department of Neurobiology, Department of Physiology and Pathophysiology, Key Laboratory for Neurodegenerative Disorders of the Ministry of Education, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China. FAU - Song, Huimeng AU - Song H AD - Department of Neurobiology, Department of Physiology and Pathophysiology, Key Laboratory for Neurodegenerative Disorders of the Ministry of Education, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China. FAU - Wan, Rongqi AU - Wan R AD - Department of Neurobiology, Department of Physiology and Pathophysiology, Key Laboratory for Neurodegenerative Disorders of the Ministry of Education, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China. FAU - Wang, Wei AU - Wang W AD - Department of Neurobiology, Department of Physiology and Pathophysiology, Key Laboratory for Neurodegenerative Disorders of the Ministry of Education, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China. FAU - Yin, Yanling AU - Yin Y AD - Department of Neurobiology, Department of Physiology and Pathophysiology, Key Laboratory for Neurodegenerative Disorders of the Ministry of Education, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, PR China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20210304 PL - Australia TA - Theranostics JT - Theranostics JID - 101552395 RN - 0 (Qb-SNARE Proteins) RN - 0 (Qc-SNARE Proteins) RN - 0 (Receptors, AMPA) RN - 0 (Snap29 protein, mouse) RN - 0 (Snap29 protein, rat) SB - IM MH - Animals MH - Autophagy/genetics MH - Cell Survival/genetics MH - Cognitive Dysfunction/etiology/*genetics/metabolism MH - Disease Models, Animal MH - Excitatory Postsynaptic Potentials/*genetics MH - Gene Knockdown Techniques MH - Hypoglycemia MH - Hypoxia MH - In Vitro Techniques MH - Infarction, Middle Cerebral Artery MH - Ischemic Stroke/*complications MH - Mice MH - Neurons/*metabolism MH - Patch-Clamp Techniques MH - Presynaptic Terminals/*metabolism MH - Primary Cell Culture MH - Qb-SNARE Proteins/*genetics/metabolism MH - Qc-SNARE Proteins/*genetics/metabolism MH - Rats MH - Receptors, AMPA/metabolism MH - Synaptic Vesicles/*metabolism PMC - PMC7978312 OTO - NOTNLM OT - SNAP29 OT - cognitive impairment OT - ischemic stroke OT - synaptic function OT - synaptic vesicle release COIS- Competing Interests: The authors have declared that no competing interest exists. EDAT- 2021/03/24 06:00 MHDA- 2021/08/17 06:00 PMCR- 2021/01/01 CRDT- 2021/03/23 06:56 PHST- 2020/10/08 00:00 [received] PHST- 2021/02/07 00:00 [accepted] PHST- 2021/03/23 06:56 [entrez] PHST- 2021/03/24 06:00 [pubmed] PHST- 2021/08/17 06:00 [medline] PHST- 2021/01/01 00:00 [pmc-release] AID - thnov11p4616 [pii] AID - 10.7150/thno.54210 [doi] PST - epublish SO - Theranostics. 2021 Mar 4;11(10):4616-4636. doi: 10.7150/thno.54210. eCollection 2021.