PMID- 33754256
OWN - NLM
STAT- MEDLINE
DCOM- 20211108
LR  - 20211108
IS  - 1573-904X (Electronic)
IS  - 0724-8741 (Linking)
VI  - 38
IP  - 4
DP  - 2021 Apr
TI  - Ephedra Herb, Mao, Inhibits Antigen-Induced Mast Cell Degranulation by Induction 
      of the Affinity Receptor for IgE Internalization.
PG  - 569-581
LID - 10.1007/s11095-021-03020-0 [doi]
AB  - PURPOSE: Ephedra herb (Mao) exerts potent anti-allergic effects. This study aimed 
      to examine the underlying mechanisms of Mao on allergic inflammation using in 
      vitro cultured mast cells (MCs) and an in vivo model of MC-dependent anaphylaxis. 
      METHODS: Bone marrow-derived MCs (BMMCs) were presensitized with 
      anti-2,4-dinitrophenol (DNP) immunoglobulin E (IgE) and challenged with antigens 
      (Ag; DNP-human serum albumin). Degranulation responses and cell surface 
      high-affinity receptor for IgE (FcepsilonRI) expression were assessed with/without Mao 
      treatment. Passive systemic anaphylaxis (PSA)-treated mice were administered Mao 
      and the pathophysiological responses were evaluated. RESULTS: Mao inhibited 
      Ag-induced BMMC degranulation, but not polyclonal activation with phorbol 
      12-myristate 13-acetate (PMA) and ionomycin, indicating that Mao inhibits 
      IgE-dependent activation of BMMCs. Mao-treated BMMCs exhibited significant 
      reductions in expression of surface IgE and its receptor FcepsilonRI. Analysis of 
      subcellular localization revealed that Mao induces FcepsilonRI internalization in BMMCs 
      without degranulation. In the PSA mouse model, Mao administration prevented 
      antigen-induced hypothermia. Mao administration significantly reduced cell 
      surface expression of IgE-bound FcepsilonRI on peritoneal MCs. CONCLUSIONS: Mao induced 
      FcepsilonRI internalization in MCs, thereby inhibiting Ag-induced IgE-dependent 
      degranulation. The inhibitory effects of Mao on MC degranulation may offer a 
      novel therapeutic approach for allergic diseases.
FAU - Nagata, Yuka
AU  - Nagata Y
AD  - Laboratory of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Institute 
      of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi 
      Ishikawa, Kanazawa, 920-1192, Japan.
FAU - Ando, Hirokazu
AU  - Ando H
AD  - Laboratory of Molecular Pharmacognosy, Faculty of Pharmaceutical Sciences, 
      Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 
      Kanazawa, Japan.
FAU - Sasaki, Yohei
AU  - Sasaki Y
AD  - Laboratory of Molecular Pharmacognosy, Faculty of Pharmaceutical Sciences, 
      Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 
      Kanazawa, Japan.
FAU - Suzuki, Ryo
AU  - Suzuki R
AUID- ORCID: 0000-0002-9580-7266
AD  - Laboratory of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Institute 
      of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi 
      Ishikawa, Kanazawa, 920-1192, Japan. suzukir@p.kanazawa-u.ac.jp.
LA  - eng
GR  - 16H05082/Japan Society for the Promotion of Science/
PT  - Journal Article
DEP - 20210322
PL  - United States
TA  - Pharm Res
JT  - Pharmaceutical research
JID - 8406521
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Plant Extracts)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 56092-81-0 (Ionomycin)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Anaphylaxis/*drug therapy/immunology
MH  - Animals
MH  - Anti-Allergic Agents/*pharmacology/therapeutic use
MH  - Cell Degranulation/drug effects/immunology
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Ephedra/*chemistry
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/metabolism
MH  - Ionomycin/immunology
MH  - Mast Cells/*drug effects/immunology
MH  - Medicine, Kampo/methods
MH  - Mice
MH  - Plant Extracts/*pharmacology/therapeutic use
MH  - Plant Stems/chemistry
MH  - Primary Cell Culture
MH  - Signal Transduction/drug effects/immunology
MH  - Tetradecanoylphorbol Acetate/immunology
OTO - NOTNLM
OT  - FcepsilonRI
OT  - degranulation
OT  - ephedra herb
OT  - internalization
OT  - mast cell
EDAT- 2021/03/24 06:00
MHDA- 2021/11/09 06:00
CRDT- 2021/03/23 07:03
PHST- 2020/10/26 00:00 [received]
PHST- 2021/02/22 00:00 [accepted]
PHST- 2021/03/24 06:00 [pubmed]
PHST- 2021/11/09 06:00 [medline]
PHST- 2021/03/23 07:03 [entrez]
AID - 10.1007/s11095-021-03020-0 [pii]
AID - 10.1007/s11095-021-03020-0 [doi]
PST - ppublish
SO  - Pharm Res. 2021 Apr;38(4):569-581. doi: 10.1007/s11095-021-03020-0. Epub 2021 Mar 
      22.