PMID- 33755281
OWN - NLM
STAT- MEDLINE
DCOM- 20210727
LR  - 20210727
IS  - 1099-0461 (Electronic)
IS  - 1095-6670 (Linking)
VI  - 35
IP  - 6
DP  - 2021 Jun
TI  - Zerumin A attenuates the inflammatory responses in LPS-stimulated H9c2 
      cardiomyoblasts.
PG  - 1-11
LID - 10.1002/jbt.22777 [doi]
AB  - Zerumin A (ZA) is one of the potential components of Curcuma amada rhizomes, and 
      it has been shown to possess a variety of pharmacological activities. This study 
      deals with the beneficial activity of ZA in lipopolysaccharide (LPS)-stimulated 
      inflammation in H9c2 cardiomyoblasts. Herein, H9c2 cells were preincubated with 
      ZA for 1 h and stimulated with LPS for 24 h. The cells were analyzed for the 
      expression of various pro-inflammatory mediators and signaling molecules. Results 
      showed that the cell viability was significantly improved and reactive oxygen 
      species production was alleviated remarkably with ZA pretreatment. We also found 
      that ZA pretreatment significantly suppressed the upregulation of inducible 
      nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) protein levels, and nitric 
      oxide (NO) release in LPS-stimulated cells. In addition, ZA 
      significantly ameliorated LPS-elicited overexpression of pro-inflammatory 
      chemokines and cytokines such as monocyte chemoattractant protein-1 (MCP-1), 
      tumor necrosis factor alpha (TNF- alpha), interferon-gamma (IFN-gamma), and interleukin-1 (IL-1) 
      in H9c2 cells, and it upregulated the synthesis of the anti-inflammatory cytokine 
      interleukin-10 (IL-10). Moreover, pretreatment with ZA and the mitogen-activated 
      protein kinases (MAPK) pathway inhibitors also reduced the phosphorylation of 
      extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinases (JNK), and 
      p38. ZA significantly inhibited IkB-a phosphorylation and nuclear factor (NF)-kB 
      p65 subunit translocation into nuclei. Overall data demonstrated that ZA protects 
      cardiomyocytes against LPS injury by inhibiting NF-kB p65 activation via the MAPK 
      signaling pathway in vitro. These findings suggest that ZA may be a promising 
      agent for a detailed study for the prevention or treatment of myocardial 
      dysfunction in sepsis.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Shyni, G L
AU  - Shyni GL
AD  - Biochemistry and Molecular Mechanism Laboratory, Agro-Processing and Technology 
      Division, CSIR-National Institute for Interdisciplinary Science and Technology 
      (CSIR-NIIST), Thiruvananthapuram, Kerala, India.
FAU - Renjitha, J
AU  - Renjitha J
AD  - Chemical Sciences and Technology Division, CSIR-National Institute for 
      Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram, 
      Kerala, India.
AD  - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, UP, India.
FAU - B Somappa, Sasidhar
AU  - B Somappa S
AD  - Chemical Sciences and Technology Division, CSIR-National Institute for 
      Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram, 
      Kerala, India.
AD  - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, UP, India.
FAU - Raghu, K G
AU  - Raghu KG
AUID- ORCID: 0000-0002-1341-5470
AD  - Biochemistry and Molecular Mechanism Laboratory, Agro-Processing and Technology 
      Division, CSIR-National Institute for Interdisciplinary Science and Technology 
      (CSIR-NIIST), Thiruvananthapuram, Kerala, India.
AD  - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, UP, India.
LA  - eng
PT  - Journal Article
DEP - 20210323
PL  - United States
TA  - J Biochem Mol Toxicol
JT  - Journal of biochemical and molecular toxicology
JID - 9717231
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Diterpenes)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Cell Line
MH  - Cytokines/metabolism
MH  - Diterpenes/*pharmacology
MH  - Inflammation/chemically induced/drug therapy/metabolism/pathology
MH  - Lipopolysaccharides/*toxicity
MH  - MAP Kinase Signaling System/*drug effects
MH  - Myoblasts, Cardiac/*metabolism/pathology
MH  - Rats
OTO - NOTNLM
OT  - H9c2 cells
OT  - Zerumin A
OT  - cytokines
OT  - inducible nitric oxide synthase
OT  - lipopolysaccharide
EDAT- 2021/03/24 06:00
MHDA- 2021/07/28 06:00
CRDT- 2021/03/23 12:43
PHST- 2020/12/21 00:00 [revised]
PHST- 2020/09/23 00:00 [received]
PHST- 2021/03/12 00:00 [accepted]
PHST- 2021/03/24 06:00 [pubmed]
PHST- 2021/07/28 06:00 [medline]
PHST- 2021/03/23 12:43 [entrez]
AID - 10.1002/jbt.22777 [doi]
PST - ppublish
SO  - J Biochem Mol Toxicol. 2021 Jun;35(6):1-11. doi: 10.1002/jbt.22777. Epub 2021 Mar 
      23.