PMID- 33755802
OWN - NLM
STAT- MEDLINE
DCOM- 20210607
LR  - 20220716
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 166
IP  - 6
DP  - 2021 Jun
TI  - Development of a TaqMan loop-mediated isothermal amplification assay for the 
      rapid detection of pigeon paramyxovirus type 1.
PG  - 1599-1605
LID - 10.1007/s00705-021-04963-w [doi]
AB  - Pigeon paramyxovirus-1 (PPMV-1) is a strain of Newcastle disease virus (NDV) that 
      has adapted to infect pigeons and poses a constant threat to the commercial 
      poultry industry. Early detection via rapid and sensitive methods, along with 
      timely preventative and mitigating actions, is important for reducing the spread 
      of PPMV-1. Here, we report the development of a TaqMan loop-mediated isothermal 
      amplification assay (TaqMan-LAMP) for rapid and specific detection of PPMV-1 
      based on the F gene. This system makes use of six novel primers and a TaqMan 
      probe that targets nine distinct regions of the F gene that are highly conserved 
      among PPMV-1 isolates. The results showed that the limit of detection was 10 
      copies muL(-1) for PPMV-1 cDNA and 0.1 ng for PPMV-1 RNA. The reaction was 
      completed within 25 min and was thus faster than conventional RT-PCR. Moreover, 
      no cross-reactions with similar viruses or with peste des petits ruminants virus 
      (PPRV) or NDV LaSota vaccine strains were observed under the same conditions. To 
      evaluate the applicability of the assay, the TaqMan-LAMP assay and a commercial 
      RT-PCR assay were compared using 108 clinical samples, and the concordance rate 
      between two methods was found to be 96.3%. The newly developed PPMV-1 TaqMan-LAMP 
      assay can therefore be used for simple, efficient, rapid, specific, and sensitive 
      diagnosis of PPMV-1 infections.
FAU - Liang, Ruiying
AU  - Liang R
AUID- ORCID: 0000-0002-3377-4390
AD  - Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 
      100193, China.
AD  - Beijing Scientific Observation and Experimental Station of Veterinary Drugs and 
      Diagnostic Technology, Ministry of Agriculture, Beijing, 100193, China.
FAU - Liang, Lin
AU  - Liang L
AD  - Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 
      100193, China.
AD  - Beijing Scientific Observation and Experimental Station of Veterinary Drugs and 
      Diagnostic Technology, Ministry of Agriculture, Beijing, 100193, China.
FAU - Ren, Xiaoxia
AU  - Ren X
AD  - China Institute of Veterinary Drug Control, Beijing, 100081, China.
FAU - Jia, Yaxiong
AU  - Jia Y
AD  - Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 
      100193, China.
AD  - Beijing Scientific Observation and Experimental Station of Veterinary Drugs and 
      Diagnostic Technology, Ministry of Agriculture, Beijing, 100193, China.
FAU - Han, Kun
AU  - Han K
AD  - Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 
      100193, China.
AD  - Beijing Scientific Observation and Experimental Station of Veterinary Drugs and 
      Diagnostic Technology, Ministry of Agriculture, Beijing, 100193, China.
FAU - Zhao, Jingjie
AU  - Zhao J
AD  - Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 
      100193, China.
AD  - Beijing Scientific Observation and Experimental Station of Veterinary Drugs and 
      Diagnostic Technology, Ministry of Agriculture, Beijing, 100193, China.
FAU - Song, Cuiping
AU  - Song C
AD  - China Animal Health and Epidemiology Center, Qingdao, 266032, China. 
      songcuiping@cahec.cn.
FAU - Cui, Shangjin
AU  - Cui S
AD  - Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 
      100193, China. cuishangjin@caas.cn.
AD  - Beijing Scientific Observation and Experimental Station of Veterinary Drugs and 
      Diagnostic Technology, Ministry of Agriculture, Beijing, 100193, China. 
      cuishangjin@caas.cn.
LA  - eng
GR  - BAIC-2020/The Modern Agricultural Industry Technology System Beijing Poultry 
      Innovation Team/
GR  - ASTIP-IAS15/The Agricultural Science and Technology Innovation Program of China/
PT  - Journal Article
DEP - 20210323
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (RNA, Viral)
RN  - LAMP assay
SB  - IM
MH  - Animals
MH  - Columbidae
MH  - Feces/virology
MH  - Molecular Diagnostic Techniques/methods/*veterinary
MH  - Newcastle disease virus/*genetics/*isolation & purification
MH  - Nucleic Acid Amplification Techniques/methods/*veterinary
MH  - RNA, Viral
MH  - Sensitivity and Specificity
MH  - Time Factors
PMC - PMC7986176
COIS- The authors declare that they have no competing interests.
EDAT- 2021/03/24 06:00
MHDA- 2021/06/08 06:00
PMCR- 2021/03/23
CRDT- 2021/03/23 17:54
PHST- 2020/08/12 00:00 [received]
PHST- 2020/12/02 00:00 [accepted]
PHST- 2021/03/24 06:00 [pubmed]
PHST- 2021/06/08 06:00 [medline]
PHST- 2021/03/23 17:54 [entrez]
PHST- 2021/03/23 00:00 [pmc-release]
AID - 10.1007/s00705-021-04963-w [pii]
AID - 4963 [pii]
AID - 10.1007/s00705-021-04963-w [doi]
PST - ppublish
SO  - Arch Virol. 2021 Jun;166(6):1599-1605. doi: 10.1007/s00705-021-04963-w. Epub 2021 
      Mar 23.