PMID- 33755879
OWN - NLM
STAT- MEDLINE
DCOM- 20210906
LR  - 20210906
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 476
IP  - 8
DP  - 2021 Aug
TI  - Circ_0023028 contributes to the progression of laryngeal squamous cell carcinoma 
      by upregulating LASP1 through miR-486-3p.
PG  - 2951-2961
LID - 10.1007/s11010-021-04129-x [doi]
AB  - Circular RNAs (circRNAs) are implicated in the tumorigenesis of human cancers. 
      However, the effects of circRNAs on laryngeal squamous cell carcinoma (LSCC) are 
      largely unknown. Here, we aimed to explore the roles of circ_0023028 in LSCC 
      development. Quantitative real-time polymerase chain reaction (qRT-PCR) was 
      performed to measure circ_0023028, miR-486-3p, and Lin-Isl-Mec (LIM) and SH3 
      domain protein 1 (LASP1) mRNA. The characteristics of circ_0023028 were 
      determined by RNase R digestion assay and Actinomycin D assay. Cell Counting 
      Kit-8 (CCK-8) assay and colony formation assay were utilized for cell 
      proliferation. Transwell assay was adopted for cell invasion and migration. Flow 
      cytometry analysis was carried out to analyze cell cycle and apoptosis. RNA 
      pull-down assay and dual-luciferase reporter assay were used to explore the 
      association between miR-486-3p and circ_0023028 or LASP1. Western blot assay was 
      adopted to measure LASP1 protein level. Murine xenograft model was executed to 
      investigate the function of circ_0023028 in vivo. High expression of circ_0023028 
      was observed in LSCC tissues and cells. Circ_0023028 was stable and possessed a 
      loop structure. Circ_0023028 silencing suppressed LSCC cell proliferation, 
      metastasis and cell cycle process and induced apoptosis in vitro and hampered 
      tumor growth in vivo. Further mechanism analysis demonstrated that circ_0023028 
      could sponge miR-486-3p to regulate LASP1 expression in LSCC cells. The malignant 
      behaviors of LSCC cells mediated by circ_0023028 knockdown were rescued by the 
      inhibition of miR-486-3p. Moreover, miR-486-3p suppressed LSCC cell progression 
      via binding to LASP1. Circ_0023028 knockdown might impede the progression of LSCC 
      by regulating miR-486-3p/LASP1 axis, which could provide a novel insight on the 
      mechanism of LSCC progression.
FAU - Zheng, Yuebin
AU  - Zheng Y
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Zigong First People's 
      Hospital, No.42, Shangyihao Yi Zhi Road, Ziliujing, Zigong, 643000, Sichuan, 
      China.
FAU - Duan, Lifu
AU  - Duan L
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Zigong First People's 
      Hospital, No.42, Shangyihao Yi Zhi Road, Ziliujing, Zigong, 643000, Sichuan, 
      China.
FAU - Yang, Yirong
AU  - Yang Y
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Zigong First People's 
      Hospital, No.42, Shangyihao Yi Zhi Road, Ziliujing, Zigong, 643000, Sichuan, 
      China.
FAU - Luo, Dengyao
AU  - Luo D
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Zigong First People's 
      Hospital, No.42, Shangyihao Yi Zhi Road, Ziliujing, Zigong, 643000, Sichuan, 
      China.
FAU - Yan, Bincheng
AU  - Yan B
AUID- ORCID: 0000-0002-7208-3808
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Zigong First People's 
      Hospital, No.42, Shangyihao Yi Zhi Road, Ziliujing, Zigong, 643000, Sichuan, 
      China. qghwdz3@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210323
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (LASP1 protein, human)
RN  - 0 (LIM Domain Proteins)
RN  - 0 (MIRN486 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/genetics/*metabolism
MH  - Animals
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*pathology
MH  - Cell Proliferation
MH  - Cytoskeletal Proteins/genetics/*metabolism
MH  - Disease Progression
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - LIM Domain Proteins/genetics/*metabolism
MH  - Laryngeal Neoplasms/genetics/metabolism/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Prognosis
MH  - RNA, Circular/*genetics
MH  - Tumor Cells, Cultured
MH  - Xenograft Model Antitumor Assays
OTO - NOTNLM
OT  - Circ_0023028
OT  - LASP1
OT  - LSCC
OT  - MiR-486-3p
EDAT- 2021/03/24 06:00
MHDA- 2021/09/07 06:00
CRDT- 2021/03/23 17:57
PHST- 2020/09/22 00:00 [received]
PHST- 2021/03/04 00:00 [accepted]
PHST- 2021/03/24 06:00 [pubmed]
PHST- 2021/09/07 06:00 [medline]
PHST- 2021/03/23 17:57 [entrez]
AID - 10.1007/s11010-021-04129-x [pii]
AID - 10.1007/s11010-021-04129-x [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2021 Aug;476(8):2951-2961. doi: 10.1007/s11010-021-04129-x. 
      Epub 2021 Mar 23.