PMID- 33759195
OWN - NLM
STAT- MEDLINE
DCOM- 20211213
LR  - 20211214
IS  - 1749-6632 (Electronic)
IS  - 0077-8923 (Linking)
VI  - 1503
IP  - 1
DP  - 2021 Nov
TI  - Extracellular vesicles from M1-polarized macrophages promote inflammation in the 
      temporomandibular joint via miR-1246 activation of the Wnt/beta-catenin pathway.
PG  - 48-59
LID - 10.1111/nyas.14590 [doi]
AB  - Macrophage-mediated regulation of chondrocytes plays an important role in 
      promoting temporomandibular joint (TMJ) inflammation. We investigated whether 
      extracellular vesicles (EVs) derived from M1 macrophages (M1-EVs) have a 
      proinflammatory effect on TMJ inflammation and what the associated mechanisms 
      are. In vitro, purified THP-1 cell-derived M1-EVs were applied to human TMJ 
      condylar chondrocytes, and in vivo M1-EVs derived from bone marrow-derived 
      macrophages (BMDMs) were injected into rat TMJs. The levels of IL-6, IL-8, IL-1beta, 
      and matrix metalloproteinase were then evaluated and found to be upregulated in 
      the chondrocytes and rat TMJs. MicroRNA sequencing analysis was performed to 
      identify differential expression of miRNAs, including miR-1246. High expression 
      of miR-1246 in M1-EVs from synovial fluid of patients with TMJ osteoarthritis and 
      synovitis was verified by RT-PCR. We then identified miR-1246 targets GSK3beta and 
      Axin2 and found that miR-1246 inhibits GSK3beta and Axin2 expression, causing 
      activation of the Wnt/beta-catenin pathway and inflammation in condylar 
      chondrocytes. Our study found that M1-EVs promote inflammation by transfer of 
      miR-1246 to condylar chondrocytes, thus providing new insight into one mechanism 
      that can promote TMJ inflammation.
CI  - (c) 2021 New York Academy of Sciences.
FAU - Peng, Sisi
AU  - Peng S
AD  - College of Stomatology, Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing, 
      China.
AD  - Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher 
      Education, Chongqing, China.
FAU - Yan, Yin
AU  - Yan Y
AD  - College of Stomatology, Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing, 
      China.
AD  - Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher 
      Education, Chongqing, China.
FAU - Li, Rui
AU  - Li R
AD  - College of Stomatology, Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing, 
      China.
AD  - Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher 
      Education, Chongqing, China.
FAU - Dai, Hongwei
AU  - Dai H
AD  - College of Stomatology, Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing, 
      China.
AD  - Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher 
      Education, Chongqing, China.
FAU - Xu, Jie
AU  - Xu J
AD  - College of Stomatology, Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing, 
      China.
AD  - Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher 
      Education, Chongqing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210323
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (MIRN1246 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Arthritis/*etiology/*metabolism/pathology
MH  - Biomarkers
MH  - Cytokines/metabolism
MH  - Disease Susceptibility
MH  - Extracellular Vesicles/*metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Inflammation Mediators
MH  - Macrophage Activation/genetics/immunology
MH  - Macrophages/immunology/*metabolism
MH  - MicroRNAs/*genetics
MH  - Temporomandibular Joint/*pathology
MH  - *Wnt Signaling Pathway
OTO - NOTNLM
OT  - M1
OT  - Wnt/beta-catenin
OT  - extracellular vesicles
OT  - inflammation
OT  - macrophage
OT  - microRNA
OT  - temporomandibular joint
EDAT- 2021/03/25 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/03/24 07:02
PHST- 2021/02/17 00:00 [revised]
PHST- 2020/10/28 00:00 [received]
PHST- 2021/02/24 00:00 [accepted]
PHST- 2021/03/25 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/03/24 07:02 [entrez]
AID - 10.1111/nyas.14590 [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2021 Nov;1503(1):48-59. doi: 10.1111/nyas.14590. Epub 2021 Mar 
      23.