PMID- 33762200
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20240214
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 154
DP  - 2021 May
TI  - Defining chaperone-usher fimbriae repertoire in Serratia marcescens.
PG  - 104857
LID - S0882-4010(21)00129-7 [pii]
LID - 10.1016/j.micpath.2021.104857 [doi]
AB  - Chaperone-usher (CU) fimbriae are surface organelles particularly prevalent among 
      the Enterobacteriaceae. Mainly associated to their adhesive properties, CU 
      fimbriae play key roles in biofilm formation and host cell interactions. Little 
      is known about the fimbriome composition of the opportunistic human pathogen 
      Serratia marcescens. Here, by using a search based on consensus fimbrial usher 
      protein (FUP) sequences, we identified 421 FUPs across 39 S. marcescens genomes. 
      Further analysis of the FUP-containing loci allowed us to classify them into 20 
      conserved CU operons, 6 of which form the S. marcescens core CU fimbriome. A new 
      systematic nomenclature is proposed according to FUP sequence phylogeny. We also 
      established an in vivo transcriptional assay comparing CU promoter expression 
      between an environmental and a clinical isolate of S. marcescens, which revealed 
      that promoters from 3 core CU operons (referred as fgov, fpo, and fps) are 
      predominantly expressed in the two strains and might represent key core adhesion 
      appendages contributing to S. marcescens pathogenesis.
CI  - Copyright (c) 2021. Published by Elsevier Ltd.
FAU - Gonzalez-Montalvo, Martin A
AU  - Gonzalez-Montalvo MA
AD  - Universidad Autonoma de Nuevo Leon, Facultad de Medicina, Departamento de 
      Microbiologia, Monterrey, Nuevo Leon, 64460, Mexico.
FAU - Tavares-Carreon, Faviola
AU  - Tavares-Carreon F
AD  - Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Biologicas, San Nicolas 
      de los Garza, Nuevo Leon, 66455, Mexico.
FAU - Gonzalez, Gloria M
AU  - Gonzalez GM
AD  - Universidad Autonoma de Nuevo Leon, Facultad de Medicina, Departamento de 
      Microbiologia, Monterrey, Nuevo Leon, 64460, Mexico.
FAU - Villanueva-Lozano, Hiram
AU  - Villanueva-Lozano H
AD  - Universidad Autonoma de Nuevo Leon, Facultad de Medicina, Departamento de 
      Microbiologia, Monterrey, Nuevo Leon, 64460, Mexico.
FAU - Garcia-Romero, Inmaculada
AU  - Garcia-Romero I
AD  - Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, 
      Belfast, BT9 7BL, United Kingdom.
FAU - Zomosa-Signoret, Viviana C
AU  - Zomosa-Signoret VC
AD  - Universidad Autonoma de Nuevo Leon, Facultad de Medicina, Departamento de 
      Bioquimica y Medicina Molecular, Monterrey, Nuevo Leon, 64460, Mexico.
FAU - Valvano, Miguel A
AU  - Valvano MA
AD  - Wellcome-Wolfson Institute for Experimental Medicine, Queen's University Belfast, 
      Belfast, BT9 7BL, United Kingdom.
FAU - Andrade, Angel
AU  - Andrade A
AD  - Universidad Autonoma de Nuevo Leon, Facultad de Medicina, Departamento de 
      Microbiologia, Monterrey, Nuevo Leon, 64460, Mexico. Electronic address: 
      angel.andradet@uanl.edu.mx.
LA  - eng
GR  - MR/P022480/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
DEP - 20210321
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Molecular Chaperones)
SB  - IM
MH  - *Fimbriae, Bacterial/genetics
MH  - Humans
MH  - Molecular Chaperones/genetics
MH  - Operon
MH  - Phylogeny
MH  - *Serratia marcescens/genetics
OTO - NOTNLM
OT  - Fimbriae
OT  - Serratia marcescens
OT  - Usher protein
EDAT- 2021/03/26 06:00
MHDA- 2021/06/22 06:00
CRDT- 2021/03/25 05:49
PHST- 2021/01/06 00:00 [received]
PHST- 2021/03/12 00:00 [accepted]
PHST- 2021/03/26 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/03/25 05:49 [entrez]
AID - S0882-4010(21)00129-7 [pii]
AID - 10.1016/j.micpath.2021.104857 [doi]
PST - ppublish
SO  - Microb Pathog. 2021 May;154:104857. doi: 10.1016/j.micpath.2021.104857. Epub 2021 
      Mar 21.