PMID- 3376422
OWN - NLM
STAT- MEDLINE
DCOM- 19880711
LR  - 20190828
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 18
IP  - 1
DP  - 1988 Feb
TI  - Generation and characterization of murine monoclonal antibodies specific for 
      bovine immunoglobulin E.
PG  - 53-66
AB  - Monoclonal antibodies were produced against serum-derived bovine immunoglobulin E 
      (IgE). Culture supernatants of hybridomas were initially screened by 
      enzyme-linked immunosorbent assay (ELISA). Supernatant-derived antibodies were 
      concentrated and further characterized using ELISA, reverse cutaneous 
      anaphylaxis, immunohistochemical staining, and immunoblotting of IgE-containing 
      samples separated by SDS-polyacrylamide gel electrophoresis (PAGE). Eight 
      monoclonal antibodies showed specificity for bovine epsilon immunoglobulin heavy 
      chain. Two antibodies (E2 and E32) reacted in immunoblots of SDS-PAGE of serum 
      IgE under reducing conditions. Additionally, E2, E5, and E32 detected epsilon 
      chain in serum separated by SDS-PAGE and then renatured. Antigen-specific IgE was 
      detected in Western blots by E5 and E32. Immunoperoxidase staining of 
      IgE-containing cells in mesenteric lymph node sections was detected with E5, E21 
      and E32. All eight antibodies produced positive reverse cutaneous anaphylaxis 
      reactions in calf skin. All functioned well in ELISA as a plate-sensitizing 
      reagent for quantitation of total IgE; E5 and E32 worked well as a primary 
      antibody in antigen-specific IgE assays. These antibodies will be useful in 
      research applications and in diagnostic assays.
FAU - Thatcher, E F
AU  - Thatcher EF
AD  - Department of Veterinary Microbiology and Immunology, School of Veterinary 
      Medicine, University of California, Davis 95616.
FAU - Gershwin, L J
AU  - Gershwin LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Monoclonal)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibody Specificity
MH  - Cattle
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoglobulin E/analysis/*immunology
MH  - Immunohistochemistry
MH  - Mice
MH  - Passive Cutaneous Anaphylaxis
EDAT- 1988/02/01 00:00
MHDA- 1988/02/01 00:01
CRDT- 1988/02/01 00:00
PHST- 1988/02/01 00:00 [pubmed]
PHST- 1988/02/01 00:01 [medline]
PHST- 1988/02/01 00:00 [entrez]
AID - 0165-2427(88)90036-0 [pii]
AID - 10.1016/0165-2427(88)90036-0 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 1988 Feb;18(1):53-66. doi: 
      10.1016/0165-2427(88)90036-0.