PMID- 33765573
OWN - NLM
STAT- MEDLINE
DCOM- 20210427
LR  - 20210427
IS  - 1095-8630 (Electronic)
IS  - 0301-4797 (Linking)
VI  - 288
DP  - 2021 Jun 15
TI  - Biodegradation and physiological response mechanism of Bacillus aryabhattai to 
      cyclotetramethylenete-tranitramine (HMX) contamination.
PG  - 112247
LID - S0301-4797(21)00309-1 [pii]
LID - 10.1016/j.jenvman.2021.112247 [doi]
AB  - This study aims to reveal the biodegradation and interaction mechanism of 
      cyclotetramethylenete-tranitramine (HMX) by a newly isolated bacteria. In this 
      study, a bacterial strain (Bacillus aryabhattai) with high efficiency for HMX 
      degradation was used as the test organism to analyze the changes in growth 
      status, cell function, and mineral metabolism following exposure to different 
      stress concentrations (0 and 5 mg L(-1)) of HMX. Non-targeted metabonomics was 
      used to reveal the metabolic response of this strain to HMX stress. The results 
      showed that when the HMX concentration was 5 mg L(-1), the removal rate of HMX 
      within 24 h of inoculation with Bacillus aryabhatta was as high as 90.5%, the 
      OD(600) turbidity was 1.024, and the BOD(5) was 225 mg L(-1). Scanning electron 
      microscope (SEM) images showed that the morphology of bacteria was not obvious 
      Variety, Fourier transform infrared spectroscopy (FTIR) showed that the cell 
      surface -OH functional groups drifted, and ICP-MS showed that the cell mineral 
      element metabolism was disturbed. Non-targeted metabonomics showed that HMX 
      induced the differential expression of 254 metabolites (133 upregulated and 221 
      downregulated). The main differentially expressed metabolites during HMX stress 
      were lipids and lipid-like molecules, and the most significantly affected 
      metabolic pathway was purine metabolism. At the same time, the primary metabolic 
      network of bacteria was disordered. These results confirmed that Bacillus 
      aryabhattai has a high tolerance to HMX and can efficiently degrade HMX. The 
      degradation mechanism involves the extracellular decomposition of HMX and 
      transformation of the degradation products into intracellular purines, amino 
      sugars, and nucleoside sugars that then participate in cell metabolism.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Yang, Xu
AU  - Yang X
AD  - School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010, China.
FAU - Lai, Jin-Long
AU  - Lai JL
AD  - School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010, China.
FAU - Li, Jie
AU  - Li J
AD  - School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010, China.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010, China. Electronic address: zhangyuustc@163.com.
FAU - Luo, Xue-Gang
AU  - Luo XG
AD  - School of Life Science and Engineering, Southwest University of Science and 
      Technology, Mianyang, 621010, China.
FAU - Han, Meng-Wei
AU  - Han MW
AD  - State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China.
FAU - Zhu, Yong-Bing
AU  - Zhu YB
AD  - National NBC National Key Laboratory of Civilian Protection, Beijing, 102205, 
      China.
FAU - Zhao, San-Ping
AU  - Zhao SP
AD  - State Key Laboratory of NBC Protection for Civilian, Beijing, 102205, China. 
      Electronic address: spzhao@mail.ustc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210322
PL  - England
TA  - J Environ Manage
JT  - Journal of environmental management
JID - 0401664
RN  - 0 (Azocines)
RN  - LLW94W5BSJ (octogen)
RN  - Bacillus aryabhattai
SB  - IM
MH  - Azocines
MH  - *Bacillus
MH  - Biodegradation, Environmental
OTO - NOTNLM
OT  - Biodegradation
OT  - HMX
OT  - Metabonomics
OT  - Microbial remediation
EDAT- 2021/03/26 06:00
MHDA- 2021/04/28 06:00
CRDT- 2021/03/25 20:18
PHST- 2020/12/20 00:00 [received]
PHST- 2021/01/27 00:00 [revised]
PHST- 2021/02/19 00:00 [accepted]
PHST- 2021/03/26 06:00 [pubmed]
PHST- 2021/04/28 06:00 [medline]
PHST- 2021/03/25 20:18 [entrez]
AID - S0301-4797(21)00309-1 [pii]
AID - 10.1016/j.jenvman.2021.112247 [doi]
PST - ppublish
SO  - J Environ Manage. 2021 Jun 15;288:112247. doi: 10.1016/j.jenvman.2021.112247. 
      Epub 2021 Mar 22.