PMID- 33768096
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210327
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 9
DP  - 2021
TI  - Activation of AMPK Promotes Maturation of Cardiomyocytes Derived From Human 
      Induced Pluripotent Stem Cells.
PG  - 644667
LID - 10.3389/fcell.2021.644667 [doi]
LID - 644667
AB  - Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CMs) 
      (hiPSC-CMs) are a promising cell source for disease modeling, myocardial 
      regeneration, and drug assessment. However, hiPSC-CMs have certain immature fetal 
      CM-like properties that are different from the characteristics of adult CMs in 
      several aspects, including cellular structure, mitochondrial function, and 
      metabolism, thus limiting their applications. Adenosine 5'-monophosphate 
      (AMP)-activated protein kinase (AMPK) is an energy-sensing protein kinase 
      involved in the regulation of fatty acid oxidation and mitochondrial biogenesis 
      in cardiomyocytes. This study investigated the effects of AMPK on the maturation 
      of hiPSC-CMs. Activation of AMPK in hiPSC-CMs significantly increased the 
      expression of CM-specific markers and resulted in a more mature myocardial 
      structure compared to that in the control cells. We found that activation of AMPK 
      improved mitochondrial oxidative phosphorylation (OxPhos) and the oxygen 
      consumption rate (OCR). Additionally, our data demonstrated that activation of 
      AMPK increased mitochondrial fusion to promote the maturation of mitochondrial 
      structure and function. Overall, activation of AMPK is an effective approach to 
      promote hiPSC-CMs maturation, which may enhance the utility of hiPSC-CMs in 
      clinical applications.
CI  - Copyright (c) 2021 Ye, Zhang, Zhou, Tan, Xu, Yi, Yan, Xie, Zhang, Tian and Zhu.
FAU - Ye, Liang
AU  - Ye L
AD  - Department of Pediatric Research Institute, Ministry of Education Key Laboratory 
      of Child Development and Disorders, National Clinical Research Center for Child 
      Health and Disorders (Chongqing), China International Science and Technology 
      Cooperation Base of Child Development and Critical Disorders, Children's Hospital 
      of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
FAU - Zhang, Xinyuan
AU  - Zhang X
AD  - Department of Pediatric Research Institute, Ministry of Education Key Laboratory 
      of Child Development and Disorders, National Clinical Research Center for Child 
      Health and Disorders (Chongqing), China International Science and Technology 
      Cooperation Base of Child Development and Critical Disorders, Children's Hospital 
      of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
FAU - Zhou, Qin
AU  - Zhou Q
AD  - Department of Pediatric Research Institute, Ministry of Education Key Laboratory 
      of Child Development and Disorders, National Clinical Research Center for Child 
      Health and Disorders (Chongqing), China International Science and Technology 
      Cooperation Base of Child Development and Critical Disorders, Children's Hospital 
      of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
FAU - Tan, Bin
AU  - Tan B
AD  - Department of Pediatric Research Institute, Ministry of Education Key Laboratory 
      of Child Development and Disorders, National Clinical Research Center for Child 
      Health and Disorders (Chongqing), China International Science and Technology 
      Cooperation Base of Child Development and Critical Disorders, Children's Hospital 
      of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
FAU - Xu, Hao
AU  - Xu H
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
AD  - Department of Clinical Laboratory, Ministry of Education Key Laboratory of Child 
      Development and Disorders, National Clinical Research Center for Child Health and 
      Disorders (Chongqing), China International Science and Technology Cooperation 
      Base of Child Development and Critical Disorders, Children's Hospital of 
      Chongqing Medical University, Chongqing, China.
FAU - Yi, Qin
AU  - Yi Q
AD  - Department of Pediatric Research Institute, Ministry of Education Key Laboratory 
      of Child Development and Disorders, National Clinical Research Center for Child 
      Health and Disorders (Chongqing), China International Science and Technology 
      Cooperation Base of Child Development and Critical Disorders, Children's Hospital 
      of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
FAU - Yan, Liang
AU  - Yan L
AD  - Department of Pediatric Research Institute, Ministry of Education Key Laboratory 
      of Child Development and Disorders, National Clinical Research Center for Child 
      Health and Disorders (Chongqing), China International Science and Technology 
      Cooperation Base of Child Development and Critical Disorders, Children's Hospital 
      of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
FAU - Xie, Min
AU  - Xie M
AD  - Department of Pediatric Research Institute, Ministry of Education Key Laboratory 
      of Child Development and Disorders, National Clinical Research Center for Child 
      Health and Disorders (Chongqing), China International Science and Technology 
      Cooperation Base of Child Development and Critical Disorders, Children's Hospital 
      of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
FAU - Zhang, Yin
AU  - Zhang Y
AD  - Department of Pediatric Research Institute, Ministry of Education Key Laboratory 
      of Child Development and Disorders, National Clinical Research Center for Child 
      Health and Disorders (Chongqing), China International Science and Technology 
      Cooperation Base of Child Development and Critical Disorders, Children's Hospital 
      of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
FAU - Tian, Jie
AU  - Tian J
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
AD  - Department of Cardiovascular (Internal Medicine), Ministry of Education Key 
      Laboratory of Child Development and Disorders, National Clinical Research Center 
      for Child Health and Disorders (Chongqing), China International Science and 
      Technology Cooperation Base of Child Development and Critical Disorders, 
      Children's Hospital of Chongqing Medical University, Chongqing, China.
FAU - Zhu, Jing
AU  - Zhu J
AD  - Department of Pediatric Research Institute, Ministry of Education Key Laboratory 
      of Child Development and Disorders, National Clinical Research Center for Child 
      Health and Disorders (Chongqing), China International Science and Technology 
      Cooperation Base of Child Development and Critical Disorders, Children's Hospital 
      of Chongqing Medical University, Chongqing, China.
AD  - Chongqing Key Laboratory of Pediatrics, Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20210309
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC7985185
OTO - NOTNLM
OT  - AMPK
OT  - cardiomyocyte maturation
OT  - hiPSC-CMs
OT  - hiPSCs
OT  - mitochondrial
EDAT- 2021/03/27 06:00
MHDA- 2021/03/27 06:01
PMCR- 2021/01/01
CRDT- 2021/03/26 07:00
PHST- 2020/12/21 00:00 [received]
PHST- 2021/02/18 00:00 [accepted]
PHST- 2021/03/26 07:00 [entrez]
PHST- 2021/03/27 06:00 [pubmed]
PHST- 2021/03/27 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fcell.2021.644667 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2021 Mar 9;9:644667. doi: 10.3389/fcell.2021.644667. 
      eCollection 2021.