PMID- 33771629
OWN - NLM
STAT- MEDLINE
DCOM- 20210617
LR  - 20210617
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 154
DP  - 2021 May
TI  - Paracoccidioidesbrasiliensis induces IL-32 and is controlled by 
      IL-15/IL-32/vitamin D pathway in vitro.
PG  - 104864
LID - S0882-4010(21)00136-4 [pii]
LID - 10.1016/j.micpath.2021.104864 [doi]
AB  - Paracoccidioidomycosis (PCM) is a systemic fungal disease caused by 
      Paracoccidioides spp., whose clinical outcome depends on immune response. 
      Interleukin 32 (IL-32) is a cytokine present in inflammatory and infectious 
      diseases, including bacterial, virus and protozoan infections. Its role in fungal 
      disease remains unclear. The axis IL-15, IL-32 and vitamin D leads to 
      microbicidal capacity against intracellular pathogens. Thus, the aims of this 
      study were to investigate the production of IL-32 during Paracoccidioides spp. 
      infection and whether this cytokine and IL-15 can increase P. brasiliensis 
      control in a vitamin D dependent manner. IL-32 was highly detected in oral 
      lesions from patients with PCM. In addition, high production of this cytokine was 
      intracellularly detected in peripheral blood mononuclear cells (PBMCs) from 
      healthy donors after exposure to particulated P. brasiliensis antigens (PbAg). 
      The IL-32gamma isoform was predominantly expressed, but there was mRNA alternative 
      splicing for IL-32alpha isoform. The induction of IL-32 was dependent on Dectin-1 
      receptor. Infection of PBMCs with P. brasiliensis yeasts did not significantly 
      induce IL-32 production even after activation with exogenous IFN-gamma or IL-15 
      treatments. Although IL-15 was a potent inducer of IL-32 production, treatment 
      with this cytokine did not increase the fungal control unless vitamin D was 
      present in high levels. In this case, both IL-15 and IL-32 increased fungicidal 
      activity of PBMCs. Together, data showed that IL-32 is present in lesions of PCM, 
      PbAg induces IL-32, and the axis of IL-15/IL-32/vitamin D can contribute to 
      control fungal infection. The data suggest that exposure to molecules from P. 
      brasiliensis, as beta-glucans, is needed to induce IL-32 production since only 
      heat-killed and sonicated P. brasiliensis yeasts were able to increase IL-32, 
      which was blocked by anti-Dectin-1 antibodies. This is the first description 
      about IL-15/IL-32/vitamin D pathway role in P. brasiliensis infection.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Guimaraes de Matos, Grazzielle
AU  - Guimaraes de Matos G
AD  - Laboratorio de Imunidade Natural (LIN), Instituto de Patologia Tropical e Saude 
      Publica, Universidade Federal de Goias, Goiania, Goias, Brazil.
FAU - Barroso de Figueiredo, Ana Marina
AU  - Barroso de Figueiredo AM
AD  - Laboratorio de Imunidade Natural (LIN), Instituto de Patologia Tropical e Saude 
      Publica, Universidade Federal de Goias, Goiania, Goias, Brazil.
FAU - Diniz Goncalves, Pedro Hugo
AU  - Diniz Goncalves PH
AD  - Laboratorio de Imunidade Natural (LIN), Instituto de Patologia Tropical e Saude 
      Publica, Universidade Federal de Goias, Goiania, Goias, Brazil.
FAU - Luiz de Lima Silva, Lucas
AU  - Luiz de Lima Silva L
AD  - Laboratorio de Imunidade Natural (LIN), Instituto de Patologia Tropical e Saude 
      Publica, Universidade Federal de Goias, Goiania, Goias, Brazil.
FAU - Bastista, Aline Carvalho
AU  - Bastista AC
AD  - Faculdade de Odontologia, Universidade Federal de Goias, Goiania, Goias, Brazil.
FAU - Borges, Clayton Luiz
AU  - Borges CL
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias (UFG), Goiania, Goias, Brazil.
FAU - Maria de Almeida Soares, Celia
AU  - Maria de Almeida Soares C
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias (UFG), Goiania, Goias, Brazil.
FAU - Joosten, Leo A B
AU  - Joosten LAB
AD  - Department of Internal Medicine and Radboud Center of Infectious Diseases (RCI), 
      Radboud University Medical Center, Nijmegen, the Netherlands.
FAU - Ribeiro-Dias, Fatima
AU  - Ribeiro-Dias F
AD  - Laboratorio de Imunidade Natural (LIN), Instituto de Patologia Tropical e Saude 
      Publica, Universidade Federal de Goias, Goiania, Goias, Brazil. Electronic 
      address: fdias@ufg.br.
LA  - eng
PT  - Journal Article
DEP - 20210323
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Interleukin-15)
RN  - 0 (Interleukins)
RN  - 1406-16-2 (Vitamin D)
SB  - IM
MH  - Humans
MH  - Interleukin-15
MH  - Interleukins
MH  - Leukocytes, Mononuclear
MH  - *Paracoccidioides
MH  - *Paracoccidioidomycosis
MH  - Vitamin D
OTO - NOTNLM
OT  - Interleukin-15
OT  - Interleukin-32
OT  - Paracoccidioides brasiliensis
OT  - Vitamin D
OT  - human mononuclear cells
EDAT- 2021/03/28 06:00
MHDA- 2021/06/22 06:00
CRDT- 2021/03/27 05:49
PHST- 2020/11/29 00:00 [received]
PHST- 2021/02/19 00:00 [revised]
PHST- 2021/03/05 00:00 [accepted]
PHST- 2021/03/28 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2021/03/27 05:49 [entrez]
AID - S0882-4010(21)00136-4 [pii]
AID - 10.1016/j.micpath.2021.104864 [doi]
PST - ppublish
SO  - Microb Pathog. 2021 May;154:104864. doi: 10.1016/j.micpath.2021.104864. Epub 2021 
      Mar 23.