PMID- 33772801
OWN - NLM
STAT- MEDLINE
DCOM- 20210610
LR  - 20220531
IS  - 1469-8137 (Electronic)
IS  - 0028-646X (Linking)
VI  - 231
IP  - 1
DP  - 2021 Jul
TI  - A plant-unique ESCRT component, FYVE4, regulates multivesicular endosome 
      biogenesis and plant growth.
PG  - 193-209
LID - 10.1111/nph.17358 [doi]
AB  - During evolution, land plants generated unique proteins that participate in 
      endosomal sorting and multivesicular endosome (MVE) biogenesis, many of them with 
      specific phosphoinositide-binding capabilities. Nonetheless, the function of most 
      plant phosphoinositide-binding proteins in endosomal trafficking remains elusive. 
      Here, we analysed several Arabidopsis mutants lacking predicted 
      phosphoinositide-binding proteins and first identified fyve4-1 as a mutant with a 
      hypersensitive response to high-boron conditions and defects in degradative 
      vacuolar sorting of membrane proteins such as the borate exporter BOR1-GFP. FYVE4 
      encodes a plant-unique, FYVE domain-containing protein that interacts with SNF7, 
      a core component of ESCRT-III (Endosomal Sorting Complex Required for Transport 
      III). FYVE4 affects the membrane association of the late-acting ESCRT components 
      SNF7 and VPS4, and modulates the formation of intraluminal vesicles (ILVs) inside 
      MVEs. The critical function of FYVE4 in the ESCRT pathway was further 
      demonstrated by the strong genetic interactions with SNF7B and LIP5. Although the 
      fyve4-1, snf7b and lip5 single mutants were viable, the fyve4-1 snf7b and fyve4-1 
      lip5 double mutants were seedling lethal, with strong defects in MVE biogenesis 
      and vacuolar sorting of ubiquitinated membrane proteins. Taken together, we 
      identified FYVE4 as a novel plant endosomal regulator, which functions in 
      ESCRTing pathway to regulate MVE biogenesis.
CI  - (c) 2021 The Authors New Phytologist (c) 2021 New Phytologist Foundation.
FAU - Liu, Chuanliang
AU  - Liu C
AUID- ORCID: 0000-0002-6860-6397
AD  - Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, 
      School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
FAU - Zeng, Yonglun
AU  - Zeng Y
AUID- ORCID: 0000-0002-9512-6487
AD  - Centre for Cell & Developmental Biology and State Key Laboratory of 
      Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, 
      Shatin, New Territories, Hong Kong, China.
FAU - Li, Hongbo
AU  - Li H
AUID- ORCID: 0000-0002-5654-1928
AD  - Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, 
      School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
FAU - Yang, Chao
AU  - Yang C
AD  - Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, 
      School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
FAU - Shen, Wenjin
AU  - Shen W
AD  - Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, 
      School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
FAU - Xu, Min
AU  - Xu M
AD  - Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, 
      School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
FAU - Xiao, Zhidan
AU  - Xiao Z
AD  - Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, 
      School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
FAU - Chen, Tongsheng
AU  - Chen T
AD  - MOE Key Laboratory & Guangdong Provincial Key Laboratory of Laser Life Science, 
      College of Biophotonics, South China Normal University, Guangzhou, 510631, China.
FAU - Li, Baiying
AU  - Li B
AD  - Centre for Cell & Developmental Biology and State Key Laboratory of 
      Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, 
      Shatin, New Territories, Hong Kong, China.
FAU - Cao, Wenhan
AU  - Cao W
AD  - Centre for Cell & Developmental Biology and State Key Laboratory of 
      Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, 
      Shatin, New Territories, Hong Kong, China.
FAU - Jiang, Liwen
AU  - Jiang L
AD  - Centre for Cell & Developmental Biology and State Key Laboratory of 
      Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, 
      Shatin, New Territories, Hong Kong, China.
FAU - Otegui, Marisa S
AU  - Otegui MS
AD  - Department of Botany, Center for Quantitative Cell Imaging, University of 
      Wisconsin-Madison, Madison, WI, 53706, USA.
FAU - Gao, Caiji
AU  - Gao C
AUID- ORCID: 0000-0003-3958-4499
AD  - Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, 
      School of Life Sciences, South China Normal University, Guangzhou, 510631, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20210422
PL  - England
TA  - New Phytol
JT  - The New phytologist
JID - 9882884
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Endosomal Sorting Complexes Required for Transport)
SB  - IM
MH  - *Arabidopsis/genetics/metabolism
MH  - Arabidopsis Proteins/*genetics
MH  - *Endosomal Sorting Complexes Required for Transport/genetics/metabolism
MH  - Endosomes/metabolism
MH  - Plant Development
MH  - Protein Transport
MH  - Vacuoles/metabolism
OTO - NOTNLM
OT  - ESCRT
OT  - FYVE domain proteins
OT  - endosomal sorting
OT  - membrane proteins
OT  - multivesicular endosome
OT  - vacuolar degradation
EDAT- 2021/03/28 06:00
MHDA- 2021/06/11 06:00
CRDT- 2021/03/27 17:26
PHST- 2021/01/04 00:00 [received]
PHST- 2021/03/18 00:00 [accepted]
PHST- 2021/03/28 06:00 [pubmed]
PHST- 2021/06/11 06:00 [medline]
PHST- 2021/03/27 17:26 [entrez]
AID - 10.1111/nph.17358 [doi]
PST - ppublish
SO  - New Phytol. 2021 Jul;231(1):193-209. doi: 10.1111/nph.17358. Epub 2021 Apr 22.