PMID- 33774143
OWN - NLM
STAT- MEDLINE
DCOM- 20220207
LR  - 20220207
IS  - 1873-2747 (Electronic)
IS  - 0361-9230 (Linking)
VI  - 171
DP  - 2021 Jun
TI  - LncRNA p21, downregulating miR-181b, aggravates neuropathic pain by upregulating 
      Tnfaip1 and inhibit the AKT/CREB axis.
PG  - 150-161
LID - S0361-9230(21)00075-7 [pii]
LID - 10.1016/j.brainresbull.2021.03.005 [doi]
AB  - Recently, there is evidence that long non-coding RNA p21 may play a regulatory 
      role in the development of neuropathic pain (NPP), but it remains to be studied. 
      In this study, we found that lncRNA p21 and tumor necrosis factor alpha-induced 
      protein 1 (Tnfaip1) expression were up-regulated and miR-181b expression was 
      down-regulated in lipopolysaccharide (LPS)-induced and activated BV-2 microglia. 
      The results of flow cytometry and ELISA suggested that overexpression of lncRNA 
      p21 or Tnfaip1 promoted apoptosis and inflammatory factors secretion, and 
      miR-181b overexpression inhibited apoptosis and secretion of inflammatory 
      factors. Luciferase reporter gene analysis validated the adsorption of miR-181b 
      by lncRNA p21. In addition, the targeting relationship between miR-181b and 
      Tnfaip1 was determined. Next, the up-regulation of lncRNA p21 and miR-181b was 
      used as a reversal experiment, and the results suggested that the up-regulation 
      of miR-181b attenuated the promoting effect of lncRNA p21 and Tnfaip1 on 
      apoptosis and inflammatory response, which may be related to the activation of 
      AKT/cAMP response element binding protein (CREB) axis. Finally, the rat model of 
      SNL with lncRNA p21 knockdown was constructed, and the results of paw retraction 
      mechanical threshold (PWMT) and paw retraction thermal latency (PWTL) 
      measurements showed that knockdown of lncRNA p21 alleviated neuropathic pain in 
      rats. In conclusion, our study found that the lncRNA p21/miR-181b/Tnfaip1 axis 
      probably plays an important role in the progression of neuropathic pain, among 
      which lncRNA p21 may become a new insight in the treatment of neuropathic pain.
CI  - Copyright (c) 2021. Published by Elsevier Inc.
FAU - Liu, Yan
AU  - Liu Y
AD  - Department of Anesthesiology, China-Japan Union Hospital of Jilin University, 
      Changchun, 130033, Jilin, China.
FAU - Sun, Hai
AU  - Sun H
AD  - Department of Anesthesiology, China-Japan Union Hospital of Jilin University, 
      Changchun, 130033, Jilin, China.
FAU - Sun, Yan
AU  - Sun Y
AD  - Department of Anesthesiology, China-Japan Union Hospital of Jilin University, 
      Changchun, 130033, Jilin, China. Electronic address: yansun@jlu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210324
PL  - United States
TA  - Brain Res Bull
JT  - Brain research bulletin
JID - 7605818
RN  - 0 (Carrier Proteins)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (MIRN181 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (TNFAIP1 protein, rat)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Line
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Inflammation/genetics/*metabolism
MH  - Male
MH  - Mice
MH  - MicroRNAs/genetics/*metabolism
MH  - Microglia/metabolism
MH  - Neuralgia/genetics/*metabolism
MH  - Pain Threshold/physiology
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Up-Regulation
OTO - NOTNLM
OT  - Neuropathic pain
OT  - The AKT/CREB axis
OT  - Tnfaip1
OT  - lncRNA p21
OT  - miR-181b
EDAT- 2021/03/29 06:00
MHDA- 2022/02/08 06:00
CRDT- 2021/03/28 20:40
PHST- 2020/10/28 00:00 [received]
PHST- 2021/03/04 00:00 [revised]
PHST- 2021/03/08 00:00 [accepted]
PHST- 2021/03/29 06:00 [pubmed]
PHST- 2022/02/08 06:00 [medline]
PHST- 2021/03/28 20:40 [entrez]
AID - S0361-9230(21)00075-7 [pii]
AID - 10.1016/j.brainresbull.2021.03.005 [doi]
PST - ppublish
SO  - Brain Res Bull. 2021 Jun;171:150-161. doi: 10.1016/j.brainresbull.2021.03.005. 
      Epub 2021 Mar 24.