PMID- 33774873
OWN - NLM
STAT- MEDLINE
DCOM- 20210719
LR  - 20210719
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 35
IP  - 5
DP  - 2021 May
TI  - Macrophage migration inhibitory factor promotes the migration of dendritic cells 
      through CD74 and the activation of the Src/PI3K/myosin II pathway.
PG  - e21418
LID - 10.1096/fj.202001605R [doi]
AB  - Constitutively expressed by innate immune cells, the cytokine macrophage 
      migration inhibitory factor (MIF) initiates host immune responses and drives 
      pathogenic responses in infectious, inflammatory, and autoimmune diseases. 
      Dendritic cells (DCs) express high levels of MIF, but the role of MIF in DC 
      function remains poorly characterized. As migration is critical for DC immune 
      surveillance, we investigated whether MIF promoted the migration of DCs. In 
      classical transwell experiments, MIF(-/-) bone marrow-derived DCs (BMDCs) or 
      MIF(+/+) BMDCs treated with ISO-1, an inhibitor of MIF, showed markedly reduced 
      spontaneous migration and chemotaxis. CD74(-/-) BMDCs that are deficient in the 
      ligand-binding component of the cognate MIF receptor exhibited a migration defect 
      similar to that of MIF(-/-) BMDCs. Adoptive transfer experiments of LPS-matured 
      MIF(+/+) and MIF(-/-) and of CD74(+/+) and CD74(-/-) BMDCs injected into the hind 
      footpads of homologous or heterologous mice showed that the autocrine and 
      paracrine MIF activity acting via CD74 contributed to the recruitment of DCs to 
      the draining lymph nodes. Mechanistically, MIF activated the Src/PI3K signaling 
      pathway and myosin II complexes, which were required for the migration of BMDCs. 
      Altogether, these data show that the cytokine MIF exerts chemokine-like activity 
      for DC motility and trafficking.
CI  - (c) 2021 The Authors. The FASEB Journal published by Wiley Periodicals LLC on 
      behalf of Federation of American Societies for Experimental Biology.
FAU - Ives, Annette
AU  - Ives A
AD  - Infectious Diseases Service, Department of Medicine, Lausanne University Hospital 
      and University of Lausanne, Lausanne, Switzerland.
FAU - Le Roy, Didier
AU  - Le Roy D
AD  - Infectious Diseases Service, Department of Medicine, Lausanne University Hospital 
      and University of Lausanne, Lausanne, Switzerland.
FAU - Theroude, Charlotte
AU  - Theroude C
AD  - Infectious Diseases Service, Department of Medicine, Lausanne University Hospital 
      and University of Lausanne, Lausanne, Switzerland.
FAU - Bernhagen, Jurgen
AU  - Bernhagen J
AUID- ORCID: 0000-0003-2996-2652
AD  - Chair of Vascular Biology, Institute for Stroke and Dementia Research (ISD), 
      Klinikum der Universitat Munchen (KUM), Ludwig-Maximilians-University (LMU), 
      Munich, Germany.
AD  - Munich Cluster for Systems Neurology (SyNergy), Munich, Germany.
FAU - Roger, Thierry
AU  - Roger T
AUID- ORCID: 0000-0002-9358-0109
AD  - Infectious Diseases Service, Department of Medicine, Lausanne University Hospital 
      and University of Lausanne, Lausanne, Switzerland.
FAU - Calandra, Thierry
AU  - Calandra T
AUID- ORCID: 0000-0003-3051-1285
AD  - Infectious Diseases Service, Department of Medicine, Lausanne University Hospital 
      and University of Lausanne, Lausanne, Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Antigens, Differentiation, B-Lymphocyte)
RN  - 0 (Chemokines)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Macrophage Migration-Inhibitory Factors)
RN  - 0 (invariant chain)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 3.6.1.- (Myosin Type II)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.2.1 (Mif protein, mouse)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation, B-Lymphocyte/*physiology
MH  - Cells, Cultured
MH  - Chemokines/metabolism
MH  - *Chemotaxis
MH  - Dendritic Cells/cytology/*physiology
MH  - Histocompatibility Antigens Class II/*physiology
MH  - Immunity
MH  - Intramolecular Oxidoreductases/genetics/*metabolism
MH  - Macrophage Migration-Inhibitory Factors/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Myosin Type II/genetics/*metabolism
MH  - Phosphatidylinositol 3-Kinases/genetics/*metabolism
MH  - Signal Transduction
MH  - src-Family Kinases/genetics/*metabolism
OTO - NOTNLM
OT  - MIF
OT  - chemokine
OT  - cytokine
OT  - dendritic cell
OT  - innate immunity
OT  - motility
EDAT- 2021/03/29 06:00
MHDA- 2021/07/20 06:00
CRDT- 2021/03/28 21:14
PHST- 2021/01/14 00:00 [revised]
PHST- 2020/06/26 00:00 [received]
PHST- 2021/01/21 00:00 [accepted]
PHST- 2021/03/28 21:14 [entrez]
PHST- 2021/03/29 06:00 [pubmed]
PHST- 2021/07/20 06:00 [medline]
AID - 10.1096/fj.202001605R [doi]
PST - ppublish
SO  - FASEB J. 2021 May;35(5):e21418. doi: 10.1096/fj.202001605R.