PMID- 33776992
OWN - NLM
STAT- MEDLINE
DCOM- 20210623
LR  - 20210623
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 12
DP  - 2021
TI  - Tenascin-C Deficiency Is Associated With Reduced Bacterial Outgrowth During 
      Klebsiella pneumoniae-Evoked Pneumosepsis in Mice.
PG  - 600979
LID - 10.3389/fimmu.2021.600979 [doi]
LID - 600979
AB  - Tenascin C (TNC) is an extracellular matrix glycoprotein that recently emerged as 
      an immunomodulator. TNC-deficient (TNC(-/-)) mice were reported to have a reduced 
      inflammatory response upon systemic administration of lipopolysaccharide, the 
      toxic component of gram-negative bacteria. Here, we investigated the role of TNC 
      during gram-negative pneumonia derived sepsis. TNC(+/+) and TNC(-/-) mice were 
      infected with Klebsiella pneumoniae via the airways and sacrificed 24 and 42 h 
      thereafter for further analysis. Pulmonary TNC protein levels were elevated 42 h 
      after infection in TNC(+/+) mice and remained undetectable in TNC(-/-) mice. 
      TNC(-/-) mice showed modestly lower bacterial loads in lungs and blood, and a 
      somewhat reduced local-but not systemic-inflammatory response. Moreover, TNC(-/-) 
      and TNC(+/+) mice did not differ with regard to neutrophil recruitment, lung 
      pathology or plasma markers of distal organ injury. These results suggest that 
      while TNC shapes the immune response during lipopolysaccharide-induced 
      inflammation, this role may be superseded during pneumosepsis caused by a common 
      gram-negative pathogen.
CI  - Copyright (c) 2021 Meijer, de Vos, Scicluna, Roelofs, Abou Faycal, Orend, Uhel and 
      van der Poll.
FAU - Meijer, Mariska T
AU  - Meijer MT
AD  - Center for Experimental and Molecular Medicine, Amsterdam University Medical 
      Centers, Location Academic Medical Center, University of Amsterdam, Amsterdam, 
      Netherlands.
AD  - Amsterdam Institute for Infection and Immunity, Amsterdam University Medical 
      Centers, Amsterdam, Netherlands.
FAU - de Vos, Alex F
AU  - de Vos AF
AD  - Center for Experimental and Molecular Medicine, Amsterdam University Medical 
      Centers, Location Academic Medical Center, University of Amsterdam, Amsterdam, 
      Netherlands.
AD  - Amsterdam Institute for Infection and Immunity, Amsterdam University Medical 
      Centers, Amsterdam, Netherlands.
FAU - Scicluna, Brendon P
AU  - Scicluna BP
AD  - Center for Experimental and Molecular Medicine, Amsterdam University Medical 
      Centers, Location Academic Medical Center, University of Amsterdam, Amsterdam, 
      Netherlands.
AD  - Amsterdam Institute for Infection and Immunity, Amsterdam University Medical 
      Centers, Amsterdam, Netherlands.
AD  - Clinical Epidemiology Biostatistics and Bioinformatics, Amsterdam University 
      Medical Centers, Location Academic Medical Center, University of Amsterdam, 
      Amsterdam, Netherlands.
FAU - Roelofs, Joris J
AU  - Roelofs JJ
AD  - Department of Pathology, Amsterdam University Medical Centers, Location Academic 
      Medical Center, University of Amsterdam, Amsterdam, Netherlands.
FAU - Abou Faycal, Cherine
AU  - Abou Faycal C
AD  - The Tumor Microenvironment Laboratory, INSERM UMR_S 1109, Universite Strasbourg, 
      Faculte de Medecine, Hopital Civil, Institut d'Hematologie et d'Immunologie, 
      Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.
FAU - Orend, Gertraud
AU  - Orend G
AD  - The Tumor Microenvironment Laboratory, INSERM UMR_S 1109, Universite Strasbourg, 
      Faculte de Medecine, Hopital Civil, Institut d'Hematologie et d'Immunologie, 
      Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.
FAU - Uhel, Fabrice
AU  - Uhel F
AD  - Center for Experimental and Molecular Medicine, Amsterdam University Medical 
      Centers, Location Academic Medical Center, University of Amsterdam, Amsterdam, 
      Netherlands.
AD  - Amsterdam Institute for Infection and Immunity, Amsterdam University Medical 
      Centers, Amsterdam, Netherlands.
FAU - van der Poll, Tom
AU  - van der Poll T
AD  - Center for Experimental and Molecular Medicine, Amsterdam University Medical 
      Centers, Location Academic Medical Center, University of Amsterdam, Amsterdam, 
      Netherlands.
AD  - Amsterdam Institute for Infection and Immunity, Amsterdam University Medical 
      Centers, Amsterdam, Netherlands.
AD  - Division of Infectious Diseases, Amsterdam University Medical Centers, Location 
      Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210311
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Tenascin)
RN  - 0 (Tnc protein, mouse)
SB  - IM
MH  - Animals
MH  - Klebsiella Infections/genetics/*immunology/pathology
MH  - Klebsiella pneumoniae/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Pneumonia, Bacterial/genetics/*immunology/pathology
MH  - Sepsis/genetics/*immunology/pathology
MH  - Tenascin/*deficiency/immunology
PMC - PMC7990887
OTO - NOTNLM
OT  - Klebsiella pneumoniae (K. pneumoniae)
OT  - alarmins
OT  - immune system
OT  - innate immunity
OT  - mice
OT  - pneumonia
OT  - sepsis
OT  - tenascin C
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/03/30 06:00
MHDA- 2021/06/24 06:00
PMCR- 2021/01/01
CRDT- 2021/03/29 06:24
PHST- 2020/08/31 00:00 [received]
PHST- 2021/02/22 00:00 [accepted]
PHST- 2021/03/29 06:24 [entrez]
PHST- 2021/03/30 06:00 [pubmed]
PHST- 2021/06/24 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2021.600979 [doi]
PST - epublish
SO  - Front Immunol. 2021 Mar 11;12:600979. doi: 10.3389/fimmu.2021.600979. eCollection 
      2021.