PMID- 33778243
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210330
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 6
IP  - 11
DP  - 2021 Mar 23
TI  - Polymer Brush-GaAs Interface and Its Use as an Antibody-Compatible Platform for 
      Biosensing.
PG  - 7286-7295
LID - 10.1021/acsomega.0c04954 [doi]
AB  - Despite evidence showing that polymer brushes (PBs) are a powerful tool used in 
      biosensing for minimizing nonspecific interactions, allowing for optimization of 
      biosensing performance, and the fact that GaAs semiconductors have proven to have 
      a remarkable potential for sensitive biomolecule detection, the combination of 
      these two robust components has never been considered nor evaluated as a platform 
      for biosensing applications. This work reports different methodologies to prepare 
      and tune PBs on the GaAs interface (PB-GaAs) and their potential as useful 
      platforms for antibody grafting, with the ultimate goal of demonstrating the 
      innovative and attractive character of the PB-GaAs interfaces in the enhanced 
      capture of antibodies and control of nonspecific interactions. Three different 
      functionalization approaches were explored, one "grafting-to" and two 
      "grafting-from," in which atom transfer radical polymerization (ATRP) was 
      performed, followed by their corresponding characterizations. Demonstration of 
      the compatibility of Escherichia coli (E. coli) and Legionella pneumophila (Lp) 
      antibodies with the PB-GaAs platform compared to the results obtained with 
      conventional biosensing architectures developed for GaAs indicates the attractive 
      potential for operation of a sensitive biosensor. Furthermore, these results 
      showed that by carefully choosing the nature and preparation methodology of a 
      PB-GaAs interface, it is possible to effectively tune the affinity of 
      PB-GaAs-based sensors toward E. coli and Lp antibodies ultimately demonstrating 
      the superior specificity of the developed biosensing platform.
CI  - (c) 2021 The Authors. Published by American Chemical Society.
FAU - Marquez, Daniela T
AU  - Marquez DT
AD  - Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, 
      Universite de Sherbrooke, 3000, Boulevard de l'Universite, Sherbrooke, Quebec J1K 
      0A5, Canada.
AD  - Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, 
      Ontario K1S 5B6, Canada.
FAU - Chawich, Juliana
AU  - Chawich J
AD  - Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, 
      Universite de Sherbrooke, 3000, Boulevard de l'Universite, Sherbrooke, Quebec J1K 
      0A5, Canada.
FAU - Hassen, Walid M
AU  - Hassen WM
AD  - Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, 
      Universite de Sherbrooke, 3000, Boulevard de l'Universite, Sherbrooke, Quebec J1K 
      0A5, Canada.
FAU - Moumanis, Khalid
AU  - Moumanis K
AD  - Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, 
      Universite de Sherbrooke, 3000, Boulevard de l'Universite, Sherbrooke, Quebec J1K 
      0A5, Canada.
FAU - DeRosa, Maria C
AU  - DeRosa MC
AD  - Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, 
      Ontario K1S 5B6, Canada.
FAU - Dubowski, Jan J
AU  - Dubowski JJ
AD  - Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, 
      Universite de Sherbrooke, 3000, Boulevard de l'Universite, Sherbrooke, Quebec J1K 
      0A5, Canada.
LA  - eng
PT  - Journal Article
DEP - 20210312
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC7992090
COIS- The authors declare no competing financial interest.
EDAT- 2021/03/30 06:00
MHDA- 2021/03/30 06:01
PMCR- 2021/03/12
CRDT- 2021/03/29 06:40
PHST- 2020/10/10 00:00 [received]
PHST- 2020/12/21 00:00 [accepted]
PHST- 2021/03/29 06:40 [entrez]
PHST- 2021/03/30 06:00 [pubmed]
PHST- 2021/03/30 06:01 [medline]
PHST- 2021/03/12 00:00 [pmc-release]
AID - 10.1021/acsomega.0c04954 [doi]
PST - epublish
SO  - ACS Omega. 2021 Mar 12;6(11):7286-7295. doi: 10.1021/acsomega.0c04954. 
      eCollection 2021 Mar 23.