PMID- 33779613
OWN - NLM
STAT- MEDLINE
DCOM- 20210510
LR  - 20210510
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 169
DP  - 2021 Mar 9
TI  - Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues.
LID - 10.3791/61997 [doi]
AB  - Pretreatment is still the most expensive step in lignocellulosic biorefinery 
      processes. It must be made cost-effective by minimizing chemical requirements as 
      well as power and heat consumption and by using environment-friendly solvents. 
      Deep eutectic solvents (DESs) are key, green, and low-cost solvents in 
      sustainable biorefineries. They are transparent mixtures characterized by low 
      freezing points resulting from at least one hydrogen bond donor and one hydrogen 
      bond acceptor. Although DESs are promising solvents, it is necessary to combine 
      them with an economic heating technology, such as microwave irradiation, for 
      competitive profitability. Microwave irradiation is a promising strategy to 
      shorten the heating time and boost fractionation because it can rapidly attain 
      the appropriate temperature. The aim of this study was to develop a one-step, 
      rapid method for biomass fractionation and lignin extraction using a low-cost and 
      biodegradable solvent. In this study, a microwave-assisted DES pretreatment was 
      conducted for 60 s at 800 W, using three kinds of DESs. The DES mixtures were 
      facilely prepared from choline chloride (ChCl) and three hydrogen-bond donors 
      (HBDs): a monocarboxylic acid (lactic acid), a dicarboxylic acid (oxalic acid), 
      and urea. This pretreatment was used for biomass fractionation and lignin 
      recovery from marine residues (Posidonia leaves and aegagropile), agri-food 
      byproducts (almond shells and olive pomace), forest residues (pinecones), and 
      perennial lignocellulosic grasses (Stipa tenacissima). Further analyses were 
      conducted to determine the yield, purity, and molecular weight distribution of 
      the recovered lignin. In addition, the effect of DESs on the chemical functional 
      groups in the extracted lignin was determined by Fourier-transform infrared 
      (FTIR) spectroscopy. The results indicate that the ChCl-oxalic acid mixture 
      affords the highest lignin purity and the lowest yield. The present study 
      demonstrates that the DES-microwave process is an ultrafast, efficient, and 
      cost-competitive technology for lignocellulosic biomass fractionation.
FAU - Kammoun, Maroua
AU  - Kammoun M
AD  - Laboratory of Biomass and Green Technologies, University of Liege (Gembloux 
      Agro-Bio Tech Campus); mkammoun@doct.uliege.be.
FAU - Berchem, Thomas
AU  - Berchem T
AD  - Laboratory of Biomass and Green Technologies, University of Liege (Gembloux 
      Agro-Bio Tech Campus).
FAU - Richel, Aurore
AU  - Richel A
AD  - Laboratory of Biomass and Green Technologies, University of Liege (Gembloux 
      Agro-Bio Tech Campus).
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20210309
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 11132-73-3 (lignocellulose)
RN  - 9005-53-2 (Lignin)
SB  - IM
MH  - Animals
MH  - Lignin/*therapeutic use
EDAT- 2021/03/30 06:00
MHDA- 2021/05/11 06:00
CRDT- 2021/03/29 13:28
PHST- 2021/03/29 13:28 [entrez]
PHST- 2021/03/30 06:00 [pubmed]
PHST- 2021/05/11 06:00 [medline]
AID - 10.3791/61997 [doi]
PST - epublish
SO  - J Vis Exp. 2021 Mar 9;(169). doi: 10.3791/61997.