PMID- 33779853 OWN - NLM STAT- MEDLINE DCOM- 20211220 LR - 20211220 IS - 1573-4838 (Electronic) IS - 0957-4530 (Print) IS - 0957-4530 (Linking) VI - 32 IP - 4 DP - 2021 Mar 29 TI - A composite scaffold of Wharton's jelly and chondroitin sulphate loaded with human umbilical cord mesenchymal stem cells repairs articular cartilage defects in rat knee. PG - 36 LID - 10.1007/s10856-021-06506-w [doi] LID - 36 AB - To evaluate the performance of a composite scaffold of Wharton's jelly (WJ) and chondroitin sulfate (CS) and the effect of the composite scaffold loaded with human umbilical cord mesenchymal stem cells (hUCMSCs) in repairing articular cartilage defects, two experiments were carried out. The in vitro experiments involved identification of the hUCMSCs, construction of the biomimetic composite scaffolds by the physical and chemical crosslinking of WJ and CS, and testing of the biomechanical properties of both the composite scaffold and the WJ scaffold. In the in vivo experiments, composite scaffolds loaded with hUCMSCs and WJ scaffolds loaded with hUCMSCs were applied to repair articular cartilage defects in the rat knee. Moreover, their repair effects were evaluated by the unaided eye, histological observations, and the immunogenicity of scaffolds and hUCMSCs. We found that in vitro, the Young's modulus of the composite scaffold (WJ-CS) was higher than that of the WJ scaffold. In vivo, the composite scaffold loaded with hUCMSCs repaired rat cartilage defects better than did the WJ scaffold loaded with hUCMSCs. Both the scaffold and hUCMSCs showed low immunogenicity. These results demonstrate that the in vitro construction of a human-derived WJ-CS composite scaffold enhances the biomechanical properties of WJ and that the repair of knee cartilage defects in rats is better with the composite scaffold than with the single WJ scaffold if the scaffold is loaded with hUCMSCs. FAU - Li, Zhong AU - Li Z AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. FAU - Bi, Yikang AU - Bi Y AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. FAU - Wu, Qi AU - Wu Q AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. FAU - Chen, Chao AU - Chen C AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. FAU - Zhou, Lu AU - Zhou L AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. FAU - Qi, Jianhong AU - Qi J AUID- ORCID: 0000-0002-8674-016X AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. jhqi7281@163.com. AD - Clinical Center for Sports Medicine and Rehabilitation, the Affiliated Hospital of Shandong First Medical University, 706 Taishan Great Street, Taian, 271000, Shandong, PR China. jhqi7281@163.com. FAU - Xie, Di AU - Xie D AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. FAU - Song, Hongqiang AU - Song H AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. FAU - Han, Yunning AU - Han Y AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. FAU - Qu, Pengwei AU - Qu P AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. FAU - Zhang, Kaihong AU - Zhang K AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. FAU - Wu, Yadi AU - Wu Y AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. FAU - Yin, Qipu AU - Yin Q AD - Institute of Sports Medicine, Shandong First Medical University & Shandong Academy Medical Sciences, 619 Changcheng Road, Taian, 271016, Shandong, PR China. LA - eng GR - ZR2017LH018/Shandong Provincial Natural Science Foundation of China/ GR - ZR2017LH018/Shandong Provincial Natural Science Foundation of China/ PT - Journal Article DEP - 20210329 PL - United States TA - J Mater Sci Mater Med JT - Journal of materials science. Materials in medicine JID - 9013087 RN - 0 (Interleukin-6) RN - 9007-28-7 (Chondroitin Sulfates) SB - IM MH - Animals MH - Biomechanical Phenomena MH - Cartilage MH - Cartilage, Articular/*metabolism MH - Cell Differentiation MH - Chondrocytes/cytology MH - Chondroitin Sulfates/*chemistry MH - Hindlimb/*physiology MH - Immunohistochemistry MH - In Vitro Techniques MH - Interleukin-6/metabolism MH - Male MH - Mesenchymal Stem Cells/*cytology MH - Rats MH - Rats, Sprague-Dawley MH - Tissue Engineering MH - Tissue Scaffolds/*chemistry MH - Umbilical Cord/*metabolism MH - Wharton Jelly/*chemistry PMC - PMC8007499 COIS- The authors declare no competing interests. EDAT- 2021/03/30 06:00 MHDA- 2021/12/21 06:00 PMCR- 2021/03/29 CRDT- 2021/03/29 13:38 PHST- 2019/07/02 00:00 [received] PHST- 2021/03/09 00:00 [accepted] PHST- 2021/03/29 13:38 [entrez] PHST- 2021/03/30 06:00 [pubmed] PHST- 2021/12/21 06:00 [medline] PHST- 2021/03/29 00:00 [pmc-release] AID - 10.1007/s10856-021-06506-w [pii] AID - 6506 [pii] AID - 10.1007/s10856-021-06506-w [doi] PST - epublish SO - J Mater Sci Mater Med. 2021 Mar 29;32(4):36. doi: 10.1007/s10856-021-06506-w.