PMID- 33781899 OWN - NLM STAT- MEDLINE DCOM- 20220126 LR - 20220716 IS - 1522-9653 (Electronic) IS - 1063-4584 (Print) IS - 1063-4584 (Linking) VI - 29 IP - 7 DP - 2021 Jul TI - Synovial fluid concentrations of matrix Metalloproteinase-3 and Interluekin-6 following anterior cruciate ligament injury associate with gait biomechanics 6 months following reconstruction. PG - 1006-1019 LID - S1063-4584(21)00670-1 [pii] LID - 10.1016/j.joca.2021.03.014 [doi] AB - OBJECTIVE: To compare gait biomechanics 6 months following anterior cruciate ligament (ACL) reconstruction (ACLR) between patients with the highest and lowest concentrations of synovial fluid (SF) interleukin-6 (IL-6) and matrix metalloproteinase-3 (MMP-3), as well as compared to uninjured controls. DESIGN: SF concentrations of IL-6 and MMP-3 were collected 7 +/- 4 days post injury in 38 ACL injured patients (55% female, 21+/-4yrs, 25.3 +/- 5.2BMI). ACL injured individuals were stratified into the lowest and highest quartiles based on IL-6 (IL-6(Lowest) and IL-6(Highest)) and MMP-3 (MMP-3(Lowest) and MMP-3(Highest)) concentrations. Gait biomechanics were collected on the injured limb 6 months post-ACLR and in 38 uninjured controls (50% female, 21+/-3yrs, 23.8 +/- 2.8BMI). Functional analyses of variance were used to compare vertical ground reaction force (vGRF), knee flexion angle (KFA), and internal knee extension moment (KEM) waveforms throughout stance phase of gait to determine the proportions of stance differing between limbs and groups. RESULTS: Compared to uninjured controls, IL-6(High) and MMP-3(High) ACL subgroups demonstrated lesser vGRF (largest differences: IL-6, 7.88%BW; MMP-3, 11.05%BW) during early-stance and greater vGRF (largest differences: IL-6, 6.21%BW; MMP-3, 5.85%BW) in mid-stance, lesser KFA (largest differences: IL-6, 3.11 degrees ; MMP-3, 3.72 degrees ) and lesser KEM (largest differences: IL-6, 0.96%BW*m; MMP-3, 1.07%BW*m) in early-stance, as well as greater KFA in mid-stance (largest differences: IL-6, 1.5 degrees ; MMP-3, 2.95 degrees ). CONCLUSIONS: High SF concentrations of a proinflammatory cytokine and a degradative enzyme early post-ACL injury are associated with aberrant gait biomechanics in the injured limb at 6 months post-ACLR (i.e., lesser vGRF, KFA and KEM) linked to posttraumatic osteoarthritis development. CI - Copyright (c) 2021 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved. FAU - Evans-Pickett, A AU - Evans-Pickett A AD - MOTION Science Institute, Department of Exercise and Sport Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Human Movement Science Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Electronic address: alyssa7evans@unc.edu. FAU - Longobardi, L AU - Longobardi L AD - Thurston Arthritis Research Center, University of North Carolina at Chapel Hill, 27599, USA. FAU - Spang, J T AU - Spang JT AD - Department of Orthopaedics, School of Medicine, University of North Carolina at Chapel Hill, NC, 27599, USA. FAU - Creighton, R A AU - Creighton RA AD - Department of Orthopaedics, School of Medicine, University of North Carolina at Chapel Hill, NC, 27599, USA. FAU - Kamath, G AU - Kamath G AD - Department of Orthopaedics, School of Medicine, University of North Carolina at Chapel Hill, NC, 27599, USA. FAU - Davis-Wilson, H C AU - Davis-Wilson HC AD - MOTION Science Institute, Department of Exercise and Sport Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Human Movement Science Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. FAU - Loeser, R AU - Loeser R AD - Thurston Arthritis Research Center, University of North Carolina at Chapel Hill, 27599, USA. FAU - Blackburn, J T AU - Blackburn JT AD - MOTION Science Institute, Department of Exercise and Sport Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Human Movement Science Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Orthopaedics, School of Medicine, University of North Carolina at Chapel Hill, NC, 27599, USA. FAU - Pietrosimone, B AU - Pietrosimone B AD - MOTION Science Institute, Department of Exercise and Sport Science, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Human Movement Science Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Department of Orthopaedics, School of Medicine, University of North Carolina at Chapel Hill, NC, 27599, USA. LA - eng GR - R03 AR066840/AR/NIAMS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20210327 PL - England TA - Osteoarthritis Cartilage JT - Osteoarthritis and cartilage JID - 9305697 RN - 0 (Interleukin-6) RN - EC 3.4.24.17 (MMP3 protein, human) RN - EC 3.4.24.17 (Matrix Metalloproteinase 3) SB - IM MH - Anterior Cruciate Ligament Injuries/physiopathology/*surgery MH - Biomechanical Phenomena/physiology MH - Case-Control Studies MH - Female MH - Gait/*physiology MH - Humans MH - Interleukin-6/*metabolism MH - Male MH - Matrix Metalloproteinase 3/*metabolism MH - Synovial Fluid/*metabolism MH - Young Adult PMC - PMC8658576 MID - NIHMS1694717 OTO - NOTNLM OT - ACL OT - Biomarkers OT - Inflammation OT - Knee OT - Posttraumatic osteoarthritis EDAT- 2021/03/31 06:00 MHDA- 2022/01/27 06:00 PMCR- 2022/07/01 CRDT- 2021/03/30 06:24 PHST- 2020/04/17 00:00 [received] PHST- 2021/03/11 00:00 [revised] PHST- 2021/03/15 00:00 [accepted] PHST- 2021/03/31 06:00 [pubmed] PHST- 2022/01/27 06:00 [medline] PHST- 2021/03/30 06:24 [entrez] PHST- 2022/07/01 00:00 [pmc-release] AID - S1063-4584(21)00670-1 [pii] AID - 10.1016/j.joca.2021.03.014 [doi] PST - ppublish SO - Osteoarthritis Cartilage. 2021 Jul;29(7):1006-1019. doi: 10.1016/j.joca.2021.03.014. Epub 2021 Mar 27.