PMID- 33783698
OWN - NLM
STAT- MEDLINE
DCOM- 20210929
LR  - 20210929
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 193
IP  - 8
DP  - 2021 Aug
TI  - Preparation of Functionalized Mesoporous Silica as a Novel Carrier and 
      Immobilization of Laccase.
PG  - 2547-2566
LID - 10.1007/s12010-021-03556-2 [doi]
AB  - Amino-modified mesoporous silica (SBA-15-NH(2)) was prepared by hydrothermal 
      method, which is a kind of excellent carrier for enzyme immobilization. The 
      structure of SBA-15 was characterized by SEM and FTIR, which proved that amino 
      group was successfully attached to the surface of SBA-15. The carrier had good 
      mesoporous structure proved by nitrogen adsorption and desorption test. Using 
      SBA-15-NH(2) as the carrier, the optimal conditions of laccase immobilization by 
      two different cross-linking methods were explored. At the same time, the 
      properties of the immobilized enzyme and free enzyme were compared. The results 
      showed that the activity of immobilized laccase by two-step method (2977.5 U/g) 
      was much higher than that by one-step method (239.5 U/g). The optimal conditions 
      were as follows: free laccase (35 degrees C, pH=4.5), two-step immobilized laccase (40 degrees C, 
      pH=4.0), one-step immobilized laccase (35 degrees C, pH=4.0). The two-step method was 
      more adaptable to temperature. The pH adaptation range of the immobilized enzyme 
      is wider, and the thermal stability is greatly enhanced. After five cycles of 
      repeated reaction, the residual enzyme activity of two-step and one-step methods 
      was 56% and 43% of the original. The treatment of simulated wastewater containing 
      2,4-dichlorophenol (2,4-DCP) by immobilized laccase was also studied. Under 
      optimum conditions (40 degrees C, pH=5.0, 20 mg/L), the removal of 2,4-DCP reached 
      89.06%. The immobilized laccase is really effective for treatment of 
      2,4-DCP-containing wastewater.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Yang, Bo
AU  - Yang B
AD  - College of Environment and Safety Engineering, Qingdao University of Science & 
      Technology, Qingdao, 266042, Shandong, China. yangbo16@126.com.
FAU - Tang, Kun
AU  - Tang K
AD  - College of Environment and Safety Engineering, Qingdao University of Science & 
      Technology, Qingdao, 266042, Shandong, China.
FAU - Wei, Shuwei
AU  - Wei S
AD  - College of Environment and Safety Engineering, Qingdao University of Science & 
      Technology, Qingdao, 266042, Shandong, China.
FAU - Zhai, Xuejun
AU  - Zhai X
AD  - College of Environment and Safety Engineering, Qingdao University of Science & 
      Technology, Qingdao, 266042, Shandong, China.
FAU - Nie, Nanzhu
AU  - Nie N
AD  - College of Environment and Safety Engineering, Qingdao University of Science & 
      Technology, Qingdao, 266042, Shandong, China.
LA  - eng
GR  - 2016YFC0208100/National key R & D projects of China/
PT  - Journal Article
DEP - 20210330
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Fungal Proteins)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - EC 1.10.3.2 (Laccase)
SB  - IM
MH  - Enzymes, Immobilized/*chemistry
MH  - Fungal Proteins/*chemistry
MH  - Laccase/*chemistry
MH  - Porosity
MH  - Silicon Dioxide/*chemistry
OTO - NOTNLM
OT  - 2,4-DCP
OT  - Cross-linking
OT  - Immobilization
OT  - Laccase
OT  - SBA-15-NH2
OT  - Water treatment
EDAT- 2021/03/31 06:00
MHDA- 2021/09/30 06:00
CRDT- 2021/03/30 12:36
PHST- 2021/02/02 00:00 [received]
PHST- 2021/03/22 00:00 [accepted]
PHST- 2021/03/31 06:00 [pubmed]
PHST- 2021/09/30 06:00 [medline]
PHST- 2021/03/30 12:36 [entrez]
AID - 10.1007/s12010-021-03556-2 [pii]
AID - 10.1007/s12010-021-03556-2 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2021 Aug;193(8):2547-2566. doi: 
      10.1007/s12010-021-03556-2. Epub 2021 Mar 30.