PMID- 33784492
OWN - NLM
STAT- MEDLINE
DCOM- 20211103
LR  - 20221116
IS  - 1744-5116 (Electronic)
IS  - 1388-0209 (Print)
IS  - 1388-0209 (Linking)
VI  - 59
IP  - 1
DP  - 2021 Dec
TI  - Effect of Lupinus rotundiflorus gamma conglutin treatment on JNK1 gene expression 
      and protein activation in a rat model of type 2 diabetes.
PG  - 374-380
LID - 10.1080/13880209.2021.1893757 [doi]
AB  - CONTEXT: Gamma conglutin (Cgamma) from lupine species represents a potential 
      complementary treatment for type 2 diabetes mellitus (T2DM) because of its 
      hypoglycaemic effect. However, its underlying mechanism of action is not fully 
      known. OBJECTIVE: To evaluate whether Cgamma from Lupinus rotundiflorus M. E. Jones 
      (Fabaceae) modulates c-Jun N-terminal kinase 1 (JNK1) expression and activation 
      in a T2DM rat model. MATERIALS AND METHODS: Gamma conglutin isolated from L. 
      rotundiflorus seeds was characterized by SDS-PAGE. Fifteen Wistar rats with 
      streptozotocin-induced T2DM (HG) were randomized into three groups (n = 5): 
      vehicle administration (HG-Ctrl), oral treatment with Cgamma (120 mg/kg/day) (HG-Lr) 
      for one week, and treatment with metformin (300 mg/kg/day) (HG-Met); a healthy 
      group (Ctrl, n = 5) was included as control. The levels of glucose and biomarkers 
      of renal and hepatic function were measured pre- and post-treatment. Hepatic Jnk1 
      expression and phosphorylation of JNK1 were evaluated by qRT-PCR and western 
      blot, respectively. RESULTS: Oral treatment with either Cgamma or metformin reduced 
      serum glucose level to 86.30 and 74.80 mg/dL, respectively (p < 0.05), from the 
      basal levels. Jnk1 expression was 0.65- and 0.54-fold lower (p < 0.05) in the 
      HG-Lr and HG-Met groups, respectively, than in HG-Ctrl. Treatment with Cgamma 
      decreased JNK1 phosphorylation. However, Cgamma did not change the levels of kidney 
      and liver biomarkers. DISCUSSION AND CONCLUSIONS: Treatment with Cgamma from L. 
      rotundiflorus inhibited Jnk1 expression, in vivo, suggesting JNK1 as a potential 
      therapeutic target in diabetes and revealing one mechanism underlying the 
      hypoglycaemic effect of lupine Cgamma. Nevertheless, further studies are required.
FAU - Zepeda-Pena, Andrea Catalina
AU  - Zepeda-Pena AC
AUID- ORCID: 0000-0003-1714-0213
AD  - Instituto de Investigacion en Enfermedades Cronico Degenerativas, Departamento de 
      Biologia Molecular y Genomica, Centro Universitario de Ciencias de la Salud, 
      Universidad de Guadalajara, Guadalajara, Mexico.
FAU - Gurrola-Diaz, Carmen Magdalena
AU  - Gurrola-Diaz CM
AUID- ORCID: 0000-0002-9851-8961
AD  - Instituto de Investigacion en Enfermedades Cronico Degenerativas, Departamento de 
      Biologia Molecular y Genomica, Centro Universitario de Ciencias de la Salud, 
      Universidad de Guadalajara, Guadalajara, Mexico.
FAU - Dominguez-Rosales, Jose Alfredo
AU  - Dominguez-Rosales JA
AUID- ORCID: 0000-0002-8560-5855
AD  - Instituto de Investigacion en Enfermedades Cronico Degenerativas, Departamento de 
      Biologia Molecular y Genomica, Centro Universitario de Ciencias de la Salud, 
      Universidad de Guadalajara, Guadalajara, Mexico.
FAU - Garcia-Lopez, Pedro Macedonio
AU  - Garcia-Lopez PM
AUID- ORCID: 0000-0001-9551-6875
AD  - Departamento de Botanica y Zoologia, Centro Universitario de Ciencias Biologicas 
      y Agropecuarias, Universidad de Guadalajara, Zapopan, Mexico.
FAU - Pizano-Andrade, Juan Carlos
AU  - Pizano-Andrade JC
AUID- ORCID: 0000-0003-2784-322X
AD  - Departamento de Botanica y Zoologia, Centro Universitario de Ciencias Biologicas 
      y Agropecuarias, Universidad de Guadalajara, Zapopan, Mexico.
FAU - Hernandez-Nazara, Zamira Helena
AU  - Hernandez-Nazara ZH
AUID- ORCID: 0000-0003-2319-8470
AD  - Instituto de Investigacion en Enfermedades Cronico Degenerativas, Departamento de 
      Biologia Molecular y Genomica, Centro Universitario de Ciencias de la Salud, 
      Universidad de Guadalajara, Guadalajara, Mexico.
FAU - Vargas-Guerrero, Belinda
AU  - Vargas-Guerrero B
AUID- ORCID: 0000-0002-9112-5153
AD  - Instituto de Investigacion en Enfermedades Cronico Degenerativas, Departamento de 
      Biologia Molecular y Genomica, Centro Universitario de Ciencias de la Salud, 
      Universidad de Guadalajara, Guadalajara, Mexico.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Pharm Biol
JT  - Pharmaceutical biology
JID - 9812552
RN  - 0 (Blood Glucose)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Plant Proteins)
RN  - 5W494URQ81 (Streptozocin)
RN  - 9100L32L2N (Metformin)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 8)
SB  - IM
MH  - Animals
MH  - Blood Glucose/drug effects
MH  - Diabetes Mellitus, Experimental/*drug therapy/physiopathology
MH  - Diabetes Mellitus, Type 2/*drug therapy/physiopathology
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Hypoglycemic Agents/isolation & purification/pharmacology
MH  - Lupinus/*chemistry
MH  - Male
MH  - Metformin/pharmacology
MH  - Mitogen-Activated Protein Kinase 8/genetics
MH  - Plant Proteins/isolation & purification/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Streptozocin
PMC - PMC8018548
OTO - NOTNLM
OT  - Hypoglycaemic effect
OT  - insulin resistance
OT  - legume
OT  - lupine
OT  - vegetal protein
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2021/03/31 06:00
MHDA- 2021/11/04 06:00
PMCR- 2021/03/30
CRDT- 2021/03/30 20:08
PHST- 2021/03/30 20:08 [entrez]
PHST- 2021/03/31 06:00 [pubmed]
PHST- 2021/11/04 06:00 [medline]
PHST- 2021/03/30 00:00 [pmc-release]
AID - 1893757 [pii]
AID - 10.1080/13880209.2021.1893757 [doi]
PST - ppublish
SO  - Pharm Biol. 2021 Dec;59(1):374-380. doi: 10.1080/13880209.2021.1893757.