PMID- 33785557
OWN - NLM
STAT- MEDLINE
DCOM- 20220509
LR  - 20220509
IS  - 1468-3288 (Electronic)
IS  - 0017-5749 (Linking)
VI  - 71
IP  - 4
DP  - 2022 Apr
TI  - Microbiota engraftment after faecal microbiota transplantation in obese subjects 
      with type 2 diabetes: a 24-week, double-blind, randomised controlled trial.
PG  - 716-723
LID - 10.1136/gutjnl-2020-323617 [doi]
AB  - OBJECTIVE: The impact of faecal microbiota transplantation (FMT) on microbiota 
      engraftment in patients with metabolic syndrome is uncertain. We aimed to study 
      whether combining FMT with lifestyle modification could enhance the engraftment 
      of favourable microbiota in obese patients with type 2 diabetes mellitus (T2DM). 
      DESIGN: In this double-blind, randomised, placebo-controlled trial, 61 obese 
      subjects with T2DM were randomly assigned to three parallel groups: FMT plus 
      lifestyle intervention (LSI), FMT alone, or sham transplantation plus LSI every 4 
      weeks for up to week 12. FMT solution was prepared from six healthy lean donors. 
      Faecal metagenomic sequencing was performed at baseline, weeks 4, 16 and 24. The 
      primary outcome was the proportion of subjects acquiring >/=20% of microbiota from 
      lean donors at week 24. RESULTS: Proportions of subjects acquiring >/=20% of 
      lean-associated microbiota at week 24 were 100%, 88.2% and 22% in the FMT plus 
      LSI, FMT alone, and sham plus LSI groups, respectively (p<0.0001). Repeated FMTs 
      significantly increased the engraftment of lean-associated microbiota (p<0.05). 
      FMT with or without LSI increased butyrate-producing bacteria. Combining LSI and 
      FMT led to increase in Bifidobacterium and Lactobacillus compared with FMT alone 
      (p<0.05). FMT plus LSI group had reduced total and low-density lipoprotein 
      cholesterol and liver stiffness at week 24 compared with baseline (p<0.05). 
      CONCLUSION: Repeated FMTs enhance the level and duration of microbiota 
      engraftment in obese patients with T2DM. Combining lifestyle intervention with 
      FMT led to more favourable changes in recipients' microbiota and improvement in 
      lipid profile and liver stiffness. TRIAL REGISTRATION NUMBER: NCT03127696.
CI  - (c) Author(s) (or their employer(s)) 2022. No commercial re-use. See rights and 
      permissions. Published by BMJ.
FAU - Ng, Siew C
AU  - Ng SC
AUID- ORCID: 0000-0002-6850-4454
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China.
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
AD  - State Key Laboratory of Digestive Disease, LKS Institute of Health Science, 
      Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, 
      China.
AD  - Microbiota Innovation Centre (MagIC Centre), Hong Kong, China.
FAU - Xu, Zhilu
AU  - Xu Z
AUID- ORCID: 0000-0002-5552-7534
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China.
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
AD  - State Key Laboratory of Digestive Disease, LKS Institute of Health Science, 
      Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, 
      China.
AD  - Microbiota Innovation Centre (MagIC Centre), Hong Kong, China.
FAU - Mak, Joyce Wing Yan
AU  - Mak JWY
AUID- ORCID: 0000-0001-5221-7349
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China.
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
AD  - State Key Laboratory of Digestive Disease, LKS Institute of Health Science, 
      Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Yang, Keli
AU  - Yang K
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China.
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
AD  - State Key Laboratory of Digestive Disease, LKS Institute of Health Science, 
      Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Liu, Qin
AU  - Liu Q
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China.
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
AD  - State Key Laboratory of Digestive Disease, LKS Institute of Health Science, 
      Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, 
      China.
AD  - Microbiota Innovation Centre (MagIC Centre), Hong Kong, China.
FAU - Zuo, Tao
AU  - Zuo T
AUID- ORCID: 0000-0001-8450-5281
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China.
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
AD  - State Key Laboratory of Digestive Disease, LKS Institute of Health Science, 
      Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, 
      China.
AD  - Microbiota Innovation Centre (MagIC Centre), Hong Kong, China.
FAU - Tang, Whitney
AU  - Tang W
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China.
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
AD  - State Key Laboratory of Digestive Disease, LKS Institute of Health Science, 
      Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, 
      China.
AD  - Microbiota Innovation Centre (MagIC Centre), Hong Kong, China.
FAU - Lau, Louis
AU  - Lau L
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
FAU - Lui, Rashid N
AU  - Lui RN
AUID- ORCID: 0000-0003-1277-9595
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
FAU - Wong, Sunny H
AU  - Wong SH
AUID- ORCID: 0000-0002-3354-9310
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China.
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
AD  - State Key Laboratory of Digestive Disease, LKS Institute of Health Science, 
      Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Tse, Yee Kit
AU  - Tse YK
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
FAU - Li, Amy Y L
AU  - Li AYL
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China.
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
AD  - State Key Laboratory of Digestive Disease, LKS Institute of Health Science, 
      Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Cheung, Kitty
AU  - Cheung K
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China.
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
FAU - Ching, Jessica Y L
AU  - Ching JYL
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China.
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
FAU - Wong, Vincent W S
AU  - Wong VWS
AUID- ORCID: 0000-0003-2215-9410
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
AD  - State Key Laboratory of Digestive Disease, LKS Institute of Health Science, 
      Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Kong, Alice P S
AU  - Kong APS
AD  - Division of Endocrinology and Diabetes, Department of Medicine and Therapeutics, 
      The Chinese University of Hong Kong, Hong Kong, China.
FAU - Ma, Ronald C W
AU  - Ma RCW
AD  - Division of Endocrinology and Diabetes, Department of Medicine and Therapeutics, 
      The Chinese University of Hong Kong, Hong Kong, China.
FAU - Chow, Elaine Y K
AU  - Chow EYK
AUID- ORCID: 0000-0002-4147-3387
AD  - Division of Endocrinology and Diabetes, Department of Medicine and Therapeutics, 
      The Chinese University of Hong Kong, Hong Kong, China.
AD  - Phase 1 Clinical Trial Centre, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Wong, Simon K H
AU  - Wong SKH
AD  - Department of Surgery, The Chinese University of Hong Kong, Hong Kong, China.
FAU - Ho, Ivan Chak Hang
AU  - Ho ICH
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
FAU - Chan, Paul K S
AU  - Chan PKS
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China.
AD  - Department of Microbiology, The Chinese University of Hong Kong, Hong Kong, 
      China.
FAU - Chan, Francis K L
AU  - Chan FKL
AUID- ORCID: 0000-0001-7388-2436
AD  - Center for Gut Microbiota Research, Faculty of Medicine, The Chinese University 
      of Hong Kong, Hong Kong, China fklchan@cuhk.edu.hk.
AD  - Department of Medicine and Therapeutics, The Chinese University of Hong Kong, 
      Hong Kong, China.
AD  - State Key Laboratory of Digestive Disease, LKS Institute of Health Science, 
      Institute of Digestive Disease, The Chinese University of Hong Kong, Hong Kong, 
      China.
AD  - Microbiota Innovation Centre (MagIC Centre), Hong Kong, China.
LA  - eng
SI  - ClinicalTrials.gov/NCT03127696
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20210330
PL  - England
TA  - Gut
JT  - Gut
JID - 2985108R
SB  - IM
MH  - *Diabetes Mellitus, Type 2/complications/therapy
MH  - Double-Blind Method
MH  - Fecal Microbiota Transplantation
MH  - Feces
MH  - *Gastrointestinal Microbiome
MH  - Humans
MH  - Obesity/complications/microbiology/therapy
MH  - Treatment Outcome
OTO - NOTNLM
OT  - enteric bacterial microflora
OT  - lipoprotein-cholesterol
OT  - obesity
COIS- Competing interests: SCN has served as advisory board member for Pfizer, Ferring, 
      Janssen, Abbvie and speaker for Ferring, Tillotts, Menarini, Janssen, Abbvie and 
      Takeda. She has received research grants from Olympus, Ferring and Abbvie. FKLC 
      has served as advisor and Lecture Speaker for Eisai Co. Ltd, AstraZeneca, Pfizer 
      Inc, Takeda Pharmaceutical Co and Takeda (China) Holdings Co. Ltd. VW-SW has 
      served as a consultant or advisory board member for 3V-BIO, AbbVie, Allergan, 
      Boehringer Ingelheim, Center for Outcomes Research in Liver Diseases, Echosens, 
      Gilead Sciences, Hanmi Pharmaceutical, Intercept, Merck, Novartis, Novo Nordisk, 
      Perspectum Diagnostics, Pfizer, ProSciento, Sagimet Biosciences, TARGET 
      PharmaSolutions and Terns; and a speaker for AbbVie, Bristol-Myers Squibb, 
      Echosens and Gilead Sciences. He has received a grant from Gilead Sciences for 
      fatty liver research. ZX: none to declare. JWYM: none to declare. KY: none to 
      declare. QL: none to declare. TZ: none to declare. WT: none to declare. LL: none 
      to declare. RNL: none to declare. SW: none to declare. YKT: none to declare. 
      AYLL: none to declare. KC: none to declare. JYLC: none to declare. AP-SK: none to 
      declare. RM: none to declare. EC: none to declare. SKHW: none to declare. CHH: 
      none to declare. PC: none to declare.
EDAT- 2021/04/01 06:00
MHDA- 2022/05/10 06:00
CRDT- 2021/03/31 06:08
PHST- 2020/11/15 00:00 [received]
PHST- 2021/03/04 00:00 [revised]
PHST- 2021/03/08 00:00 [accepted]
PHST- 2021/04/01 06:00 [pubmed]
PHST- 2022/05/10 06:00 [medline]
PHST- 2021/03/31 06:08 [entrez]
AID - gutjnl-2020-323617 [pii]
AID - 10.1136/gutjnl-2020-323617 [doi]
PST - ppublish
SO  - Gut. 2022 Apr;71(4):716-723. doi: 10.1136/gutjnl-2020-323617. Epub 2021 Mar 30.