PMID- 33786766
OWN - NLM
STAT- MEDLINE
DCOM- 20210811
LR  - 20210811
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 28
IP  - 30
DP  - 2021 Aug
TI  - Comparative study on the bacterial diversity and antibiotic resistance genes of 
      urban landscape waters replenished by reclaimed water and surface water in Xi'an, 
      China.
PG  - 41396-41406
LID - 10.1007/s11356-021-13376-w [doi]
AB  - Pathogenic bacteria and antibiotic resistance genes (ARGs) in urban landscape 
      waters may pose a potential threat to human health. However, the investigation of 
      their occurrence in the urban landscape waters replenished by reclaimed water 
      (RW) and surface water (SW) is still insufficient. The water samples collected 
      from six urban landscape waters replenished by RW or SW were used to analyze 
      bacterial diversity using high-throughput sequencing of 16S rRNA gene and to 
      detect 18 ARGs and 2 integron-integrase genes by means of quantitative PCR array. 
      Results indicated that Proteobacteria was the dominant phylum in all six urban 
      landscape waters. The bacterial species richness was lower in urban landscape 
      waters replenished by RW than that by SW. Sulfonamide resistance genes (sulI and 
      sulIII) were the major ARGs in these urban landscape waters. No significant 
      difference in the relative abundance of sulfonamide resistance genes, 
      tetracycline resistance genes, and most of beta-lactam resistance genes was 
      observed between RW-replenished and SW-replenished urban landscape waters. By 
      contrast, the relative abundance of bla(ampC) gene and qnrA gene in 
      RW-replenished urban landscape waters was significantly higher than that in 
      SW-replenished urban landscape waters (p < 0.05), which suggested that use of RW 
      may increase the amount of specific ARGs to urban landscape waters. 
      Interestingly, among six urban landscape waters, RW-replenished urban landscape 
      waters had a relatively rich variety of ARGs (12-15 of 18 ARGs) but a low 
      relative abundance of ARGs (458.90-1944.67 copies/16S x 10(6)). The RW 
      replenishment was found to have a certain impact on the bacterial diversity and 
      prevalence of ARGs in urban landscape waters, which provide new insight into the 
      effect of RW replenishment on urban landscape waters.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Zhang, Chong-Miao
AU  - Zhang CM
AD  - Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of 
      Education, Xi'an University of Architecture and Technology, Xi'an, 710055, China. 
      cmzhang@xauat.edu.cn.
AD  - Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of 
      Architecture and Technology, Xi'an, 710055, China. cmzhang@xauat.edu.cn.
AD  - International Science and Technology Cooperation Center for Urban Alternative 
      Water Resources Development, Xi'an University of Architecture and Technology, 
      Xi'an, 710055, China. cmzhang@xauat.edu.cn.
FAU - Liang, Jie
AU  - Liang J
AD  - Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of 
      Education, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
AD  - Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of 
      Architecture and Technology, Xi'an, 710055, China.
FAU - Liu, Wan-Ying
AU  - Liu WY
AD  - Key Laboratory of Northwest Water Resource, Environment and Ecology, Ministry of 
      Education, Xi'an University of Architecture and Technology, Xi'an, 710055, China.
AD  - Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of 
      Architecture and Technology, Xi'an, 710055, China.
LA  - eng
GR  - 51578441/National Natural Science Foundation of China (CN)/
GR  - 2020ZDLNY06-07/Key Research and Development Project of Shaanxi Province/
GR  - 2017YFE0127300/National Key Research and Development Project/
PT  - Journal Article
DEP - 20210330
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - *Anti-Bacterial Agents/analysis
MH  - Bacteria/genetics
MH  - China
MH  - Drug Resistance, Microbial/genetics
MH  - *Genes, Bacterial
MH  - Humans
MH  - RNA, Ribosomal, 16S/genetics
MH  - Water/analysis
OTO - NOTNLM
OT  - Antibiotic resistance genes
OT  - Bacterial diversity
OT  - Reclaimed water
OT  - Replenishment
OT  - Surface water
OT  - Urban landscape waters
EDAT- 2021/04/01 06:00
MHDA- 2021/08/12 06:00
CRDT- 2021/03/31 06:54
PHST- 2020/09/02 00:00 [received]
PHST- 2021/03/08 00:00 [accepted]
PHST- 2021/04/01 06:00 [pubmed]
PHST- 2021/08/12 06:00 [medline]
PHST- 2021/03/31 06:54 [entrez]
AID - 10.1007/s11356-021-13376-w [pii]
AID - 10.1007/s11356-021-13376-w [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2021 Aug;28(30):41396-41406. doi: 
      10.1007/s11356-021-13376-w. Epub 2021 Mar 30.