PMID- 33786883
OWN - NLM
STAT- MEDLINE
DCOM- 20210722
LR  - 20210722
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 61
IP  - 6
DP  - 2021 Jun
TI  - Rapid generation of monocyte-derived antigen-presenting cells with dendritic 
      cell-like properties.
PG  - 1845-1855
LID - 10.1111/trf.16385 [doi]
AB  - BACKGROUND: One of the major challenges in cellular therapy is the establishment 
      and validation of simple and fast production protocols meeting good manufacturing 
      practice (GMP) requirements. Dendritic cells (DCs) are of particular therapeutic 
      interest, due to their critical role in T cell response initiation and 
      regulation. Conventional wisdom states that DC generation from monocytes is a 
      time-consuming protocol, taking up to 7-9 days. STUDY DESIGN AND METHODS: This 
      study systematically screened and validated numerous culture components and 
      conditions to identify the minimal requirements, which can give rise to 
      functional monocyte-derived antigen-presenting cells (MoAPCs) in less than 48 h 
      (36 h MoAPC). A total of 36 h MoAPCs were evaluated in terms of surface marker 
      expression, endocytic capability, and induction of antigen-specific T cell 
      expansion via flow cytometry. RESULTS: Screening of media compositions, glucose 
      concentrations, and surface marker kinetics, particularly DC-SIGN as a 
      DC-specific marker, allowed the generation of DC-like APCs in 36 h (36 h MoAPCs). 
      A total of 36 h MoAPCs displayed a similar phenotype to 48 h MoAPC and standard 
      7 d MoDCs in terms of HLA-DP,DQ,DR, CD83, and DC-SIGN expression, while CD1a was 
      preferentially expressed in standard MoDCs. Functional evaluation revealed that 
      36 h MoAPCs displayed reduced endocytosis capabilities and IL-12p70 production. 
      However, 36 h MoAPCs were able to induce T cell expansion both in an allogenic 
      and antigen-specific setting. CONCLUSION: Our results indicate that mature 36 h 
      MoAPCs possess DC-like capabilities by inducing antigen-specific T cell 
      responses. This study has important implications for the generation of DC-based 
      cellular therapies, allowing a more cost and time-efficient generation of APCs.
CI  - (c) 2021 The Authors. Transfusion published by Wiley Periodicals LLC. on behalf of 
      AABB.
FAU - Cunningham, Sarah
AU  - Cunningham S
AUID- ORCID: 0000-0001-8692-1211
AD  - Department of Transfusion Medicine and Hemostaseology, University Hospital 
      Erlangen, Erlangen, Germany.
FAU - Hackstein, Holger
AU  - Hackstein H
AUID- ORCID: 0000-0001-9722-8180
AD  - Department of Transfusion Medicine and Hemostaseology, University Hospital 
      Erlangen, Erlangen, Germany.
LA  - eng
GR  - SFB Transregio 84 Project A2 to HH/Deutsche Forschungsgemeinschaft/
GR  - reserach equipment grant #INST 410/114-1 FUGG to HH/Deutsche 
      Forschungsgemeinschaft/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210330
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Antigens)
RN  - 0 (Culture Media)
RN  - 0 (Cytokines)
SB  - IM
MH  - Antigen-Presenting Cells/*cytology/metabolism
MH  - Antigens/metabolism
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Culture Media/metabolism
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*cytology/metabolism
MH  - Humans
MH  - Monocytes/*cytology/metabolism
MH  - T-Lymphocytes/cytology/metabolism
OTO - NOTNLM
OT  - 36 h MoAPCs
OT  - 48 h MoAPC
OT  - antigen-presenting cells
OT  - monocytes
EDAT- 2021/04/01 06:00
MHDA- 2021/07/23 06:00
CRDT- 2021/03/31 06:59
PHST- 2021/03/12 00:00 [revised]
PHST- 2020/09/14 00:00 [received]
PHST- 2021/03/17 00:00 [accepted]
PHST- 2021/04/01 06:00 [pubmed]
PHST- 2021/07/23 06:00 [medline]
PHST- 2021/03/31 06:59 [entrez]
AID - 10.1111/trf.16385 [doi]
PST - ppublish
SO  - Transfusion. 2021 Jun;61(6):1845-1855. doi: 10.1111/trf.16385. Epub 2021 Mar 30.