PMID- 33788466
OWN - NLM
STAT- MEDLINE
DCOM- 20210402
LR  - 20210402
IS  - 0255-2930 (Print)
IS  - 0255-2930 (Linking)
VI  - 41
IP  - 2
DP  - 2021 Feb 12
TI  - [Effect of acupoint thread-embedding on macrophage polarization of epididymis 
      adipose tissue in obese mice].
PG  - 177-82
LID - 10.13703/j.0255-2930.20191114-0003 [doi]
AB  - OBJECTIVE: To observe the effect of acupoint thread-embedding at "Zusanli" (ST 
      36) and "Fenglong" (ST 40) on the macrophage polarization of epididymis adipose 
      tissue in obese mice, and to explore the action mechanism of acupoint 
      thread-embedding on weight control. METHODS: Among 30 male C57BL/6 mice, 10 mice 
      were randomly selected and fed with normal diet, and the remaining 20 mice were 
      fed with high-fat diet to establish the obesity model. Sixteen mice with 
      successful obesity model were randomly divided into a model group and an acupoint 
      thread-embedding group, 8 mice in each group. Eight mice were selected from mice 
      which were fed with normal diet as the normal group. On the next day of 
      successful modeling, acupoint thread-embedding was performed at "Zusanli" (ST 36) 
      and "Fenglong" (ST 40) in the acupoint thread-embedding group, once every 10 days 
      for 4 times. The body weight was recorded at 0, 8, 16, 24, 32, 40 days into 
      intervention; the level of glucose metabolism was compared after intervention; 
      the level of lipid metabolism and weight of epididymal adipose tissue were 
      compared at the end of the intervention; the mRNA expression of M1 and M2 
      macrophage-related cytokines interleukin-10 (IL-6), monocyte chemoattractant 
      protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) 
      were detected by real-time PCR; the mRNA and protein expression of M1 macrophage 
      labeled inducible nitric oxide synthase (iNOS) and M2 macrophage labeled 
      arginase-1 (Arg-1) were detected by real-time PCR and Western blot. RESULTS: 
      Compared with the normal group, the body weight at 0, 8, 16, 24, 32, 40 days into 
      intervention in the model group was increased (P<0.05); the results of glucose 
      tolerance test at 0, 30, 60, 120 min and insulin tolerance test at 0, 30, 60, 90, 
      120 min in the model group were higher than those in the normal group (P<0.05); 
      the levels of total cholesterol and triacylglycerol in the model group were 
      significantly higher than those in the normal group (P<0.001, P<0.01); the weight 
      of epididymal adipose tissue in the model group was significantly higher than 
      that in the normal group (P<0.001); the mRNA expression of IL-6, MCP-1, TNF-alpha and 
      iNOS was increased (P<0.05, P<0.01, P<0.001), that of IL-10, Arg-1 was decreased 
      (P<0.01), the protein expression of iNOS was up-regulated (P<0.01), and that of 
      Arg-1 was down-regulated (P<0.001). Compared with the model group, the body 
      weight at 16, 24, 32, 40 days into treatment in the acupoint thread-embedding 
      group was reduced (P<0.05); the results of glucose tolerance test at 30, 60, 120 
      min and insulin tolerance test at 30, 60 min in the acupoint thread-embedding 
      group were lower than those in the model group (P<0.05); the levels of total 
      cholesterol and triacylglycerol in the acupoint thread-embedding group were 
      significantly lower than those in the model group (P<0.01, P<0.05); the weight of 
      epididymal adipose tissue in the acupoint thread-embedding group was 
      significantly lower than that in the model group (P<0.01); the mRNA expression of 
      IL-6, MCP-1, TNF-alpha and iNOS was reduced (P<0.05), that of IL-10, Arg-1 was 
      increased (P<0.05), the protein expression of iNOS was down-regulated (P<0.05), 
      and that of Arg-1 was up-regulated (P<0.01). CONCLUSION: Acupoint 
      thread-embedding at "Zusanli" (ST 36) and "Fenglong" (ST 40) may play a role in 
      weight control by regulating the polarization of macrophages.
FAU - Li, Ling-Li
AU  - Li LL
AD  - Department of TCM, Fourth Hospital of Wuhan City/Puai Hospital, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan 430033, Hubei 
      Province, China.
FAU - Hu, Jin-Qing
AU  - Hu JQ
AD  - Department of TCM, Fourth Hospital of Wuhan City/Puai Hospital, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan 430033, Hubei 
      Province, China.
FAU - Zhang, Si-Yi
AU  - Zhang SY
AD  - College of Clinical Chinese Medicine, Hubei University of CM.
FAU - Xie, Yong-Hua
AU  - Xie YH
AD  - Department of TCM, Fourth Hospital of Wuhan City/Puai Hospital, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan 430033, Hubei 
      Province, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhen Jiu
JT  - Zhongguo zhen jiu = Chinese acupuncture & moxibustion
JID - 8600658
SB  - IM
MH  - *Acupuncture Points
MH  - Adipose Tissue
MH  - Animals
MH  - *Epididymis
MH  - Macrophages
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
OTO - NOTNLM
OT  - acupoint thread-embedding
OT  - epididymal fat
OT  - macrophage polarization
OT  - obesity
EDAT- 2021/04/01 06:00
MHDA- 2021/04/07 06:00
CRDT- 2021/03/31 13:45
PHST- 2021/03/31 13:45 [entrez]
PHST- 2021/04/01 06:00 [pubmed]
PHST- 2021/04/07 06:00 [medline]
AID - 10.13703/j.0255-2930.20191114-0003 [doi]
PST - ppublish
SO  - Zhongguo Zhen Jiu. 2021 Feb 12;41(2):177-82. doi: 
      10.13703/j.0255-2930.20191114-0003.