PMID- 33788928 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20221110 IS - 1938-162X (Electronic) IS - 1062-6050 (Print) IS - 1062-6050 (Linking) VI - 56 IP - 4 DP - 2021 Apr 1 TI - Reliability of 3 Urine Specific Gravity Meters for Measuring Brix and Urine Solutions at Different Temperatures. PG - 389-395 LID - 10.4085/1062-6050-0142.20 [doi] AB - CONTEXT: Urine specific gravity (USG) should be measured at room temperature (20 degrees C), but the temperature of the sample is not always considered. OBJECTIVE: To evaluate the effect of sample temperature on the measurement accuracy of a digital refractometer (DIG), manual optical refractometer (MAN), and hydrometer (HYD). DESIGN: Descriptive laboratory study. MAIN OUTCOME MEASURE(S): Urine specific gravity. RESULTS: Experiment 1 (24 Brix ( degrees Bx) samples) showed that measurements via the DIG and MAN did not differ from the reference, but HYD provided lower or inconsistent values compared with degrees Bx and was highly correlated with degrees Bx solutions (r, > = 0.89). The overall diagnostic ability of elevated USG cut-off values (>/=1.020, >/=1.025, >/=1.030) was high for all tools (area under the curve >0.92). Misclassification of samples increased from 0 to 2 at 1.020 to 1 to 3 samples at cutoffs of 1.025 and 1.030 USG. Bland-Altman analysis showed that the DIG 5 degrees C underreported slightly without reporting bias (r = -0.344, P = .13); all other plots for the DIG, MAN, and HYD showed considerably larger underreporting at higher concentrations (r = -0.21 to -0.97 with P >.02) at all temperatures. The outcomes of experiment 2 (33 fresh urine samples) using DIG 20 degrees C as the standard demonstrated only negligible differences between the DIG and MAN at all temperatures but larger differences using the HYD. CONCLUSIONS: All tools showed reporting bias compared with the degrees Bx solutions, which can affect the classification of low and high urine concentration at higher USG cutoff values, especially with a sample temperature of 37 degrees C. CI - (c) by the National Athletic Trainers' Association, Inc. FAU - Wardenaar, Floris AU - Wardenaar F AD - College of Health Solutions, Arizona State University, Phoenix. FAU - Ortega-Santos, Carmen P AU - Ortega-Santos CP AD - College of Health Solutions, Arizona State University, Phoenix. FAU - Vento, Kaila AU - Vento K AD - College of Health Solutions, Arizona State University, Phoenix. FAU - Olzinski, Stephanie AU - Olzinski S AD - College of Health Solutions, Arizona State University, Phoenix. FAU - Olig, Jason AU - Olig J AD - College of Health Solutions, Arizona State University, Phoenix. FAU - Kavouras, Stavros AU - Kavouras S AD - College of Health Solutions, Arizona State University, Phoenix. FAU - Johnston, Carol AU - Johnston C AD - College of Health Solutions, Arizona State University, Phoenix. LA - eng PT - Journal Article PL - United States TA - J Athl Train JT - Journal of athletic training JID - 9301647 SB - IM PMC - PMC8063665 OTO - NOTNLM OT - accuracy OT - hydration status OT - validation EDAT- 2021/04/01 06:00 MHDA- 2021/04/01 06:01 PMCR- 2022/04/01 CRDT- 2021/03/31 17:22 PHST- 2021/04/01 06:00 [pubmed] PHST- 2021/04/01 06:01 [medline] PHST- 2021/03/31 17:22 [entrez] PHST- 2022/04/01 00:00 [pmc-release] AID - 463248 [pii] AID - 10.4085/1062-6050-0142.20 [doi] PST - ppublish SO - J Athl Train. 2021 Apr 1;56(4):389-395. doi: 10.4085/1062-6050-0142.20.