PMID- 33789637
OWN - NLM
STAT- MEDLINE
DCOM- 20210825
LR  - 20240331
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 17
IP  - 1
DP  - 2021 Mar 31
TI  - Antimicrobial and antibiofilm potentials of cinnamon oil and silver nanoparticles 
      against Streptococcus agalactiae isolated from bovine mastitis: new avenues for 
      countering resistance.
PG  - 136
LID - 10.1186/s12917-021-02842-9 [doi]
LID - 136
AB  - BACKGROUND: Streptococcus agalactiae (S. agalactiae) is a contagious pathogen of 
      bovine mastitis. It has financial implications for the dairy cattle industry in 
      certain areas of the world. Since antimicrobial resistance increases in dairy 
      farms, natural antimicrobials from herbal origins and nanoparticles have been 
      given more attention as an alternative therapy. Hence, this study reported the 
      antimicrobial and antibiofilm potentials of cinnamon oil, silver nanoparticles 
      (AgNPs), and their combination against multidrug-resistant (MDR) S. agalactiae 
      recovered from clinical bovine mastitis in Egypt. RESULTS: Our findings revealed 
      that 73% (146/200) of the examined milk samples collected from dairy cows with 
      clinical mastitis were infected with Streptococci species. Of these, 9.59% 
      (14/146) were identified as S. agalactiae and categorized as MDR. S. agalactiae 
      isolates expressed four virulence genes (Hyl, cylE, scpB, and lmb) and 
      demonstrated an ability to produce biofilms. Cinnamon oil showed high 
      antimicrobial (MICs </=0.063 mug /mL) and antibiofilm (MBIC(50) = 4 mug/mL) 
      potentials against planktonic and biofilms of S. agalactiae isolates, 
      respectively. However, AgNPs showed reasonable antimicrobial (MICs </=16 mug/mL) and 
      relatively low antibiofilm (MBIC(50) = 64 mug/mL) activities against screened 
      isolates. Synergistic antimicrobial or additive antibiofilm interactions of 
      cinnamon oil combined with AgNPs were reported for the first time. Scanning 
      electron microscope (SEM) analysis revealed that biofilms of S. agalactiae 
      isolates treated with cinnamon oil were more seriously damaged than observed in 
      AgNPs cinnamon oil combination. Moreover, reverse transcriptase quantitative 
      polymerase chain reaction (RT-qPCR) showed that cinnamon oil exerted a remarkable 
      down-regulation of pili biosynthesis genes (pilA and pilB) and their regulator 
      (rogB) against S. agalactiae biofilms, meanwhile the AgNPs cinnamon oil 
      combination demonstrated a lower efficacy. CONCLUSIONS: This is an in vitro 
      preliminary approach that documented the antibiofilm potential of cinnamon oil 
      and the inhibitory activity of cinnamon oil and its combination with AgNPs 
      against MDR S. agalactiae recovered from clinical mastitis. Further in vivo 
      studies should be carried out in animal models to provide evidence of concept for 
      implementing these alternative candidates in the treatment of dairy farms 
      infected by streptococcal mastitis in the future.
FAU - Abd El-Aziz, Norhan K
AU  - Abd El-Aziz NK
AUID- ORCID: 0000-0001-8309-9058
AD  - Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, 
      Zagazig, Sharkia, 44511, Egypt. norhan_vet@hotmail.com.
FAU - Ammar, Ahmed M
AU  - Ammar AM
AD  - Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, 
      Zagazig, Sharkia, 44511, Egypt.
FAU - El-Naenaeey, El-Sayed Y M
AU  - El-Naenaeey EYM
AD  - Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, 
      Zagazig, Sharkia, 44511, Egypt.
FAU - El Damaty, Hend M
AU  - El Damaty HM
AD  - Department of Animal Medicine, Infectious Diseases, Faculty of Veterinary 
      Medicine, Zagazig University, Zagazig, Sharkia, Egypt.
FAU - Elazazy, Asmaa A
AU  - Elazazy AA
AD  - Abou Hamad Veterinary Organizations, Ministry of Agriculture, Abou Hamad, 
      Sharkia, Egypt.
FAU - Hefny, Ahmed A
AU  - Hefny AA
AD  - Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 
      Sharkia, Egypt.
FAU - Shaker, Asmaa
AU  - Shaker A
AD  - Department of Microbiology, Veterinary Hospital, Faculty of Veterinary Medicine, 
      University of Sadat City, Sadat City, Egypt.
FAU - Eldesoukey, Ibrahim E
AU  - Eldesoukey IE
AD  - Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary 
      Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20210331
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Oils, Volatile)
RN  - 0 (Virulence Factors)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Biofilms/drug effects
MH  - Cattle
MH  - Cinnamomum zeylanicum/chemistry
MH  - Egypt
MH  - Female
MH  - Mastitis, Bovine/microbiology
MH  - *Metal Nanoparticles
MH  - Milk/microbiology
MH  - Oils, Volatile/*pharmacology
MH  - Silver/*pharmacology
MH  - Streptococcal Infections/veterinary
MH  - Streptococcus agalactiae/*drug effects
MH  - Virulence Factors/genetics
PMC - PMC8010958
OTO - NOTNLM
OT  - Antibiofilm potential
OT  - Antimicrobial activity
OT  - Cinnamon oil
OT  - Mastitis
OT  - Silver nanoparticles
COIS- The authors declare that they have no competing interests.
EDAT- 2021/04/02 06:00
MHDA- 2021/08/26 06:00
PMCR- 2021/03/31
CRDT- 2021/04/01 05:32
PHST- 2020/04/29 00:00 [received]
PHST- 2021/03/18 00:00 [accepted]
PHST- 2021/04/01 05:32 [entrez]
PHST- 2021/04/02 06:00 [pubmed]
PHST- 2021/08/26 06:00 [medline]
PHST- 2021/03/31 00:00 [pmc-release]
AID - 10.1186/s12917-021-02842-9 [pii]
AID - 2842 [pii]
AID - 10.1186/s12917-021-02842-9 [doi]
PST - epublish
SO  - BMC Vet Res. 2021 Mar 31;17(1):136. doi: 10.1186/s12917-021-02842-9.