PMID- 33794282
OWN - NLM
STAT- MEDLINE
DCOM- 20220209
LR  - 20220209
IS  - 1873-5169 (Electronic)
IS  - 0196-9781 (Linking)
VI  - 142
DP  - 2021 Aug
TI  - Effect of diet-induced obesity on kisspeptin-neurokinin B-dynorphin A neurons in 
      the arcuate nucleus and luteinizing hormone secretion in sex hormone-primed male 
      and female rats.
PG  - 170546
LID - S0196-9781(21)00054-1 [pii]
LID - 10.1016/j.peptides.2021.170546 [doi]
AB  - Metabolic stress resulting from either lack or excess of nutrients often causes 
      infertility in both sexes. Kisspeptin-neurokinin B-dynorphin A (KNDy) neurons in 
      the arcuate nucleus (ARC) has been suggested to be a key players in reproduction 
      via direct stimulation of the pulsatile gonadotropin-releasing hormone (GnRH) and 
      subsequent gonadotropin release in mammalian species. In this study, we 
      investigated the effect of high-fat diet (HFD) on hypothalamic KNDy gene 
      expression to examine the pathogenic mechanism underlying obesity-induced 
      infertility in male and female rats. Male and female rats at 7 weeks of age were 
      fed with either a standard or HFD for 4 months. In the male rats, the HFD caused 
      a significant suppression of ARC Kiss1 and Pdyn gene expressions, but did not 
      affect the plasma luteinizing hormone (LH) levels and sizes of the morphology of 
      the testis and epididymis. In the female rats, 58% of the HFD-fed female rats 
      exhibited irregular estrous cycles, whereas the remaining rats showed regular 
      cycles. Two of the 10 rats that showed HFD-induced irregular estrous cycles 
      showed profound suppression of LH pulse frequency and the number of ARC 
      Kiss1-expressing cells, whereas the other females showed normal LH pulses and ARC 
      Kiss1 expression. Our finding shows that suppression of ARC Kiss1 expression 
      might be the initial pathological change of hypogonadotropic hypogonadism in 
      HFD-fed male rats, while the obese-related infertility in the female rats may be 
      mainly induced by KNDy-independent pathways. Taken together, ARC kisspeptin 
      neurons in male rats may be susceptible to HFD-induced obesity compared with 
      those in female rats.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Minabe, Shiori
AU  - Minabe S
AD  - Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon 
      Medical School, Tokyo, 113- 8602, Japan. Electronic address: 
      minabe.shiori@gmail.com.
FAU - Iwata, Kinuyo
AU  - Iwata K
AD  - Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon 
      Medical School, Tokyo, 113- 8602, Japan.
FAU - Tsuchida, Hitomi
AU  - Tsuchida H
AD  - Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, 
      464-8601, Japan.
FAU - Tsukamura, Hiroko
AU  - Tsukamura H
AD  - Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, 
      464-8601, Japan.
FAU - Ozawa, Hitoshi
AU  - Ozawa H
AD  - Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon 
      Medical School, Tokyo, 113- 8602, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20210329
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Kisspeptins)
RN  - 74913-18-1 (Dynorphins)
RN  - 86933-75-7 (Neurokinin B)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Animals
MH  - Arcuate Nucleus of Hypothalamus/drug effects/metabolism/*pathology
MH  - Diet, High-Fat
MH  - Dynorphins/genetics/metabolism
MH  - Female
MH  - Gonadal Steroid Hormones/*pharmacology
MH  - Hypogonadism/etiology/metabolism/*pathology
MH  - Kisspeptins/genetics/metabolism
MH  - Luteinizing Hormone/*metabolism
MH  - Male
MH  - Metabolic Diseases/*complications
MH  - Neurokinin B/genetics/metabolism
MH  - Neurons/drug effects/metabolism/*pathology
MH  - Obesity/*complications
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - metabolic
OT  - stresskisspeptinmetastingonadotropin
EDAT- 2021/04/02 06:00
MHDA- 2022/02/10 06:00
CRDT- 2021/04/01 20:10
PHST- 2020/12/28 00:00 [received]
PHST- 2021/03/13 00:00 [revised]
PHST- 2021/03/24 00:00 [accepted]
PHST- 2021/04/02 06:00 [pubmed]
PHST- 2022/02/10 06:00 [medline]
PHST- 2021/04/01 20:10 [entrez]
AID - S0196-9781(21)00054-1 [pii]
AID - 10.1016/j.peptides.2021.170546 [doi]
PST - ppublish
SO  - Peptides. 2021 Aug;142:170546. doi: 10.1016/j.peptides.2021.170546. Epub 2021 Mar 
      29.