PMID- 33794460
OWN - NLM
STAT- MEDLINE
DCOM- 20210528
LR  - 20210528
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 781
DP  - 2021 Aug 10
TI  - Effects of nonylphenol exposure on histological changes, apoptosis and 
      time-course transcriptome in gills of white shrimp Litopenaeus vannamei.
PG  - 146731
LID - S0048-9697(21)01799-X [pii]
LID - 10.1016/j.scitotenv.2021.146731 [doi]
AB  - Nonylphenol (NP) is considered as one of the persistent organic pollutants (POPs) 
      in the environment. Pacific white shrimp Litopenaeus vannamei is the predominant 
      species in China, which is frequently affected by environmental pollutants. 
      However, potential toxicity mechanism of NP in shrimp has not been 
      comprehensively studied. To explore the physiological changes and molecular 
      mechanism involved in NP exposure of shrimp, we analyzed histological 
      alterations, apoptosis and transcriptional responses of L.vannamei subjected to 
      NP. Results indicated that significant changes in the histoarchitecture of the 
      gills were observed after NP exposure for 3, 12 and 48 h. Apoptosis was also 
      detected in a time-dependent manner. Numerous differentially expressed genes 
      (DEGs) were obtained at 3 h, 12 h and 48 h after exposure. On the basis of the 
      expression patterns over the time course, these DEGs were classified into 12 
      clusters. GO and KEGG enrichment analysis of these DEGs was carried out and a 
      dynamic and global view was obtained in shrimp after NP exposure on a 
      transcriptome level. In addition, 15 DEGs involved in immune response, apoptosis, 
      DNA repair, osmoregulation etc. were selected for qRT-PCR validation. The 
      expression patterns of these DEGs kept a well consistent with the high-throughput 
      data at different timepoints, which confirmed the accuracy and reliability of the 
      transcriptome data. All the results demonstrated that NP exposure might lead to 
      impairments of biological functions in gills, alter immune and antioxidant 
      response, compromise DNA repair and anti-apoptosis abilities of shrimp, cause 
      severe histopathological changes and eventually trigger apoptosis. The present 
      study enriched the information on the toxicity mechanism of crustaceans in 
      response to NP exposure.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Guo, Hui
AU  - Guo H
AD  - College of Fisheries, Guangdong Ocean University, Zhanjiang 524025, China; 
      Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for 
      Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic 
      Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524025, 
      China.
FAU - Liang, Zhi
AU  - Liang Z
AD  - College of Fisheries, Guangdong Ocean University, Zhanjiang 524025, China; 
      Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for 
      Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic 
      Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524025, 
      China.
FAU - Zheng, Peihua
AU  - Zheng P
AD  - Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical 
      Agricultural Sciences, Haikou 571101, China.
FAU - Li, Ling
AU  - Li L
AD  - College of Fisheries, Guangdong Ocean University, Zhanjiang 524025, China; 
      Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for 
      Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic 
      Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524025, 
      China.
FAU - Xian, Jian'an
AU  - Xian J
AD  - Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical 
      Agricultural Sciences, Haikou 571101, China.
FAU - Zhu, Xiaowen
AU  - Zhu X
AD  - College of Fisheries, Guangdong Ocean University, Zhanjiang 524025, China; 
      Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for 
      Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic 
      Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524025, 
      China. Electronic address: zhuxwen@gdou.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210325
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Phenols)
RN  - 79F6A2ILP5 (nonylphenol)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - China
MH  - Gills
MH  - *Penaeidae/genetics
MH  - Phenols
MH  - Reproducibility of Results
MH  - *Transcriptome
OTO - NOTNLM
OT  - Apoptosis
OT  - Histological changes
OT  - Litopenaeus vannamei
OT  - Nonylphenol
OT  - Transcriptome
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/04/02 06:00
MHDA- 2021/05/29 06:00
CRDT- 2021/04/01 20:15
PHST- 2021/01/31 00:00 [received]
PHST- 2021/03/19 00:00 [revised]
PHST- 2021/03/21 00:00 [accepted]
PHST- 2021/04/02 06:00 [pubmed]
PHST- 2021/05/29 06:00 [medline]
PHST- 2021/04/01 20:15 [entrez]
AID - S0048-9697(21)01799-X [pii]
AID - 10.1016/j.scitotenv.2021.146731 [doi]
PST - ppublish
SO  - Sci Total Environ. 2021 Aug 10;781:146731. doi: 10.1016/j.scitotenv.2021.146731. 
      Epub 2021 Mar 25.