PMID- 33795813
OWN - NLM
STAT- MEDLINE
DCOM- 20211116
LR  - 20211116
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Apr 1
TI  - Uric acid shown to contribute to increased oxidative stress level independent of 
      xanthine oxidoreductase activity in MedCity21 health examination registry.
PG  - 7378
LID - 10.1038/s41598-021-86962-0 [doi]
LID - 7378
AB  - Uric acid has both antioxidant and pro-oxidant properties in vitro by scavenging 
      and production of reactive oxygen species (ROS). This cross-sectional study 
      examined whether uric acid possesses effects on oxidative stress under 
      physiological conditions independent of xanthine oxidoreductase (XOR), which is 
      involved in uric acid and ROS production. Serum uric acid level was measured, 
      while plasma XOR activity was determined using our high-sensitive assay in 192 
      participants (91 males, 101 females) who underwent health examinations and were 
      not taking an antihyperuricemic agent. For antioxidant potential and oxidative 
      stress level, biological antioxidant potential (BAP) and derivative of reactive 
      oxygen metabolites (d-ROMs) in serum, respectively, were measured. Median uric 
      acid level and plasma XOR activity were 5.6 mg/dL and 26.1 pmol/h/mL, 
      respectively, and BAP and d-ROMs levels were 2112.8 mumol/L and 305.5 Carr U, 
      respectively. Multivariable regression analyses revealed no significant 
      association of serum uric acid level with BAP level, whereas serum uric acid 
      level showed a significant association with d-ROMs level independent of plasma 
      XOR activity (p = 0.045), which was prominent in females (p = 0.036; p for 
      interaction = 0.148). Uric acid might contribute to increased oxidative stress 
      independent of XOR activity by increasing ROS production, without affecting ROS 
      scavenging, especially in females.
FAU - Kurajoh, Masafumi
AU  - Kurajoh M
AD  - Department of Metabolism, Endocrinology, and Molecular Medicine, Osaka City 
      University Graduate School of Medicine, 1-4-3, Asahi-machi, Abeno-ku, Osaka, 
      545-8585, Japan. m1155129@med.osaka-cu.ac.jp.
FAU - Fukumoto, Shinya
AU  - Fukumoto S
AD  - Department of Premier Preventive Medicine, Osaka City University Graduate School 
      of Medicine, Osaka, Japan.
FAU - Yoshida, Shio
AU  - Yoshida S
AD  - Department of Metabolism, Endocrinology, and Molecular Medicine, Osaka City 
      University Graduate School of Medicine, 1-4-3, Asahi-machi, Abeno-ku, Osaka, 
      545-8585, Japan.
FAU - Akari, Seigo
AU  - Akari S
AD  - Department of Research and Development, Sanwa Kagaku Kenkyusho Co., Ltd., Aichi, 
      Japan.
FAU - Murase, Takayo
AU  - Murase T
AD  - Department of Research and Development, Sanwa Kagaku Kenkyusho Co., Ltd., Aichi, 
      Japan.
FAU - Nakamura, Takashi
AU  - Nakamura T
AD  - Department of Research and Development, Sanwa Kagaku Kenkyusho Co., Ltd., Aichi, 
      Japan.
FAU - Ishii, Haruka
AU  - Ishii H
AD  - Department of Medical Statistics, Osaka City University Graduate School of 
      Medicine, Osaka, Japan.
FAU - Yoshida, Hisako
AU  - Yoshida H
AD  - Department of Medical Statistics, Osaka City University Graduate School of 
      Medicine, Osaka, Japan.
FAU - Nagata, Yuki
AU  - Nagata Y
AD  - Department of Vascular Medicine, Osaka City University Graduate School of 
      Medicine, Osaka, Japan.
FAU - Morioka, Tomoaki
AU  - Morioka T
AD  - Department of Metabolism, Endocrinology, and Molecular Medicine, Osaka City 
      University Graduate School of Medicine, 1-4-3, Asahi-machi, Abeno-ku, Osaka, 
      545-8585, Japan.
FAU - Mori, Katsuhito
AU  - Mori K
AD  - Department of Nephrology, Osaka City University Graduate School of Medicine, 
      Osaka, Japan.
FAU - Imanishi, Yasuo
AU  - Imanishi Y
AD  - Department of Metabolism, Endocrinology, and Molecular Medicine, Osaka City 
      University Graduate School of Medicine, 1-4-3, Asahi-machi, Abeno-ku, Osaka, 
      545-8585, Japan.
FAU - Hirata, Kazuto
AU  - Hirata K
AD  - Osaka City University, Osaka, Japan.
FAU - Emoto, Masanori
AU  - Emoto M
AD  - Department of Metabolism, Endocrinology, and Molecular Medicine, Osaka City 
      University Graduate School of Medicine, 1-4-3, Asahi-machi, Abeno-ku, Osaka, 
      545-8585, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210401
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antioxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - 268B43MJ25 (Uric Acid)
RN  - EC 1.17.1.4 (Xanthine Dehydrogenase)
SB  - IM
MH  - Aged
MH  - Antioxidants/metabolism
MH  - Cross-Sectional Studies
MH  - Female
MH  - Health Status
MH  - Humans
MH  - Japan
MH  - Male
MH  - Metabolic Syndrome/blood
MH  - Middle Aged
MH  - Multivariate Analysis
MH  - *Oxidative Stress
MH  - Reactive Oxygen Species
MH  - Registries
MH  - Uric Acid/*blood
MH  - Xanthine Dehydrogenase/*blood
PMC - PMC8016900
COIS- Seigo Akari, Takayo Murase, and Takashi Nakamura of Sanwa Kagaku Kenkyusho Co. 
      Ltd. developed the plasma XOR activity assay used in this study and also 
      performed measurements of that activity. Their involvment does not alter our 
      adherence regarding sharing of data and materials. The other authors have no 
      conflicts of interest to declare.
EDAT- 2021/04/03 06:00
MHDA- 2021/11/17 06:00
PMCR- 2021/04/01
CRDT- 2021/04/02 06:22
PHST- 2021/02/05 00:00 [received]
PHST- 2021/03/23 00:00 [accepted]
PHST- 2021/04/02 06:22 [entrez]
PHST- 2021/04/03 06:00 [pubmed]
PHST- 2021/11/17 06:00 [medline]
PHST- 2021/04/01 00:00 [pmc-release]
AID - 10.1038/s41598-021-86962-0 [pii]
AID - 86962 [pii]
AID - 10.1038/s41598-021-86962-0 [doi]
PST - epublish
SO  - Sci Rep. 2021 Apr 1;11(1):7378. doi: 10.1038/s41598-021-86962-0.