PMID- 33797678
OWN - NLM
STAT- MEDLINE
DCOM- 20220221
LR  - 20220221
IS  - 1573-6814 (Electronic)
IS  - 1389-9333 (Linking)
VI  - 23
IP  - 1
DP  - 2022 Mar
TI  - Human laryngeal squamous cell carcinoma cell line release of endogenous 
      anandamide and 2-arachidonoylglycerol, and their antiproliferative effect via 
      exogenous supplementation: an in vitro study.
PG  - 93-100
LID - 10.1007/s10561-021-09917-9 [doi]
AB  - The level of the major endocannabinoids anandamide (AEA) and 
      2-arachidonoylglycerol (2-AG) are altered in several types of carcinomas, and are 
      known to regulate tumor growth. Thusly, this study hypothesized that the HEp-2 
      human laryngeal squamous cell carcinoma (LSCC) cell line releases AEA and 2-AG, 
      and aimed to determine if their exogenous supplementation has an 
      anti-proliferative effect in vitro. In this in vitro observational study a 
      commercial human LSCC cell line (HEp-2) was used to test for endogenous AEA and 
      2-AG release via liquid chromatography-tandem mass spectrometry (LC-MS/MS). The 
      anti-proliferative effect of AEA and 2-AG supplementation was evaluated via WST-1 
      proliferation assay. It was observed that the HEp-2 LSCC cell line released AEA 
      and 2-AG; the median quantity of AEA released was 15.69 ng mL(-1) (range: 
      14.55-15.95 ng mL(-1)) and the median quantity of 2-AG released was 2.72 ng (-1) 
      (range: 2.67-2.74 ng mL(-1)). Additionally, both AEA and 2-AG exhibited an 
      anti-proliferative effect. The anti-proliferative effect of 2-AG was stronger 
      than that of AEA. These findings suggest that AEA might function via a CB1 
      receptor-independent pathway and that 2-AG might function via a CB2-dependent 
      pathway. The present findings show that the HEp-2 LSCC cell line releases the 
      major endocannabinoids AEA and 2-AG, and that their supplementation inhibits 
      tumor cell proliferation in vitro. Thus, cannabinoid ligands might represent 
      novel drug candidates for laryngeal cancers, although functional in vivo studies 
      are required in order to validate their potency.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Onay, Ovsen
AU  - Onay O
AUID- ORCID: 0000-0002-8009-0311
AD  - Department of ENT, Faculty of Medicine, Atilim University, 06830, Golbasi, 
      Ankara, Turkey. ovsenonay8@gmail.com.
FAU - Kose, Sevil
AU  - Kose S
AUID- ORCID: 0000-0003-2188-9534
AD  - Department of Medical Biology and Genetics, Faculty of Medicine, Atilim 
      University, Ankara, Turkey.
FAU - Suslu, Nilda
AU  - Suslu N
AUID- ORCID: 0000-0001-9901-3044
AD  - Department of ENT, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
FAU - Korkusuz, Petek
AU  - Korkusuz P
AUID- ORCID: 0000-0002-7553-3915
AD  - Department of Histology and Embryology, Faculty of Medicine, Hacettepe 
      University, Ankara, Turkey.
FAU - Nemutlu, Emirhan
AU  - Nemutlu E
AUID- ORCID: 0000-0002-7337-6215
AD  - Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, 
      Ankara, Turkey.
FAU - Aydin, Canset
AU  - Aydin C
AUID- ORCID: 0000-0001-6288-636X
AD  - ENT Clinic, Medicana International Hospital, Ankara, Turkey.
FAU - Hosal, Sefik
AU  - Hosal S
AUID- ORCID: 0000-0002-1912-4287
AD  - Department of ENT, Faculty of Medicine, Atilim University, 06830, Golbasi, 
      Ankara, Turkey.
LA  - eng
GR  - Project ID: 376, Project code: 013 D10 101 004-376/Hacettepe Universitesi/
PT  - Journal Article
PT  - Observational Study
DEP - 20210402
PL  - Netherlands
TA  - Cell Tissue Bank
JT  - Cell and tissue banking
JID - 100965121
RN  - 0 (Arachidonic Acids)
RN  - 0 (Endocannabinoids)
RN  - 0 (Glycerides)
RN  - 0 (Polyunsaturated Alkamides)
RN  - 8D239QDW64 (glyceryl 2-arachidonate)
RN  - UR5G69TJKH (anandamide)
SB  - IM
MH  - Arachidonic Acids
MH  - Cell Line
MH  - Chromatography, Liquid
MH  - Dietary Supplements
MH  - *Endocannabinoids/metabolism/pharmacology
MH  - Glycerides
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - Polyunsaturated Alkamides
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - 2-Arachidonoylglycerol
OT  - Anandamide
OT  - Endocannabinoid
OT  - Laryngeal carcinoma
EDAT- 2021/04/03 06:00
MHDA- 2022/02/22 06:00
CRDT- 2021/04/02 12:22
PHST- 2020/06/03 00:00 [received]
PHST- 2021/03/22 00:00 [accepted]
PHST- 2021/04/03 06:00 [pubmed]
PHST- 2022/02/22 06:00 [medline]
PHST- 2021/04/02 12:22 [entrez]
AID - 10.1007/s10561-021-09917-9 [pii]
AID - 10.1007/s10561-021-09917-9 [doi]
PST - ppublish
SO  - Cell Tissue Bank. 2022 Mar;23(1):93-100. doi: 10.1007/s10561-021-09917-9. Epub 
      2021 Apr 2.