PMID- 33799030 OWN - NLM STAT- MEDLINE DCOM- 20210514 LR - 20210514 IS - 1873-4235 (Electronic) IS - 0956-5663 (Linking) VI - 182 DP - 2021 Jun 15 TI - AIEgens enabled ultrasensitive point-of-care test for multiple targets of food safety: Aflatoxin B(1) and cyclopiazonic acid as an example. PG - 113188 LID - S0956-5663(21)00225-6 [pii] LID - 10.1016/j.bios.2021.113188 [doi] AB - Food safety is currently a significant issue for human life and health. Various fluorescent nanomaterials have been applied in the point-of-care test (POCT) for food safety as labeling materials. However, previous fluorescent nanomaterials can cause aggregation-caused quenching (ACQ), thus reducing the detection sensitivity. Conversely, aggregation-induced emission luminogens (AIEgens) are promising candidates for POCT in the food safety field because they can enhance detection sensitivity and throughput. Mycotoxins, such as aflatoxin B(1) (AFB(1)) and cyclopiazonic acid (CPA), are a primary threat to human life and health and a significant food safety issue, and their on-site detection from farm to table is needed. Herein, an ultrasensitive point-of-care test was developed based on TPE-Br, a blue-emissive tetraphenylethylene derivative AIEgen. Under optimal conditions, this AIEgen-based lateral-flow biosensor (ALFB) allowed for a rapid response of 8 min toward AFB(1) and CPA detection, with considerable sensitivities of 0.003 and 0.01 ng/mL in peanut matrices, respectively. In peanut matrices, the recoveries were 90.3%-110.0% for both mycotoxins, with relative standard deviations (RSDs) below 6%. The ALFB was further validated via UPLC-MS/MS using spiked peanut samples. AIEgens open an avenue for on-site, ultrasensitive, high-throughput detection methods and can be extensively used in point-of-care tests in food safety. CI - Copyright (c) 2021 Elsevier B.V. All rights reserved. FAU - Hu, Xiaofeng AU - Hu X AD - National Reference Laboratory for Agricultural Testing (Biotoxin), Key Laboratory of Biology and Genetic Improvement of Oil Crops, Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China. FAU - Zhang, Pengfei AU - Zhang P AD - Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, China. FAU - Wang, Du AU - Wang D AD - National Reference Laboratory for Agricultural Testing (Biotoxin), Key Laboratory of Biology and Genetic Improvement of Oil Crops, Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China. FAU - Jiang, Jun AU - Jiang J AD - National Reference Laboratory for Agricultural Testing (Biotoxin), Key Laboratory of Biology and Genetic Improvement of Oil Crops, Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China. FAU - Chen, Xiaomei AU - Chen X AD - National Reference Laboratory for Agricultural Testing (Biotoxin), Key Laboratory of Biology and Genetic Improvement of Oil Crops, Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China. FAU - Liu, Yong AU - Liu Y AD - Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, China. FAU - Zhang, Zhaowei AU - Zhang Z AD - National Reference Laboratory for Agricultural Testing (Biotoxin), Key Laboratory of Biology and Genetic Improvement of Oil Crops, Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China. Electronic address: zwzhang@whu.edu.cn. FAU - Tang, Ben Zhong AU - Tang BZ AD - Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, China. Electronic address: tangbenz@ust.hk. FAU - Li, Peiwu AU - Li P AD - National Reference Laboratory for Agricultural Testing (Biotoxin), Key Laboratory of Biology and Genetic Improvement of Oil Crops, Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China. Electronic address: peiwuli@oilcrops.cn. LA - eng PT - Journal Article DEP - 20210320 PL - England TA - Biosens Bioelectron JT - Biosensors & bioelectronics JID - 9001289 RN - 0 (Indoles) RN - 9N2N2Y55MH (Aflatoxin B1) RN - X9TLY4580Z (cyclopiazonic acid) SB - IM MH - *Aflatoxin B1/analysis MH - *Biosensing Techniques MH - Chromatography, Liquid MH - Food Contamination/analysis MH - Humans MH - Indoles MH - Limit of Detection MH - Point-of-Care Testing MH - Tandem Mass Spectrometry OTO - NOTNLM OT - Aflatoxin B(1) OT - Aggregation-induced emission OT - Cyclopiazonic acid OT - Food safety OT - Lateral flow OT - Point-of-care test EDAT- 2021/04/03 06:00 MHDA- 2021/05/15 06:00 CRDT- 2021/04/02 20:26 PHST- 2021/01/14 00:00 [received] PHST- 2021/03/09 00:00 [revised] PHST- 2021/03/18 00:00 [accepted] PHST- 2021/04/03 06:00 [pubmed] PHST- 2021/05/15 06:00 [medline] PHST- 2021/04/02 20:26 [entrez] AID - S0956-5663(21)00225-6 [pii] AID - 10.1016/j.bios.2021.113188 [doi] PST - ppublish SO - Biosens Bioelectron. 2021 Jun 15;182:113188. doi: 10.1016/j.bios.2021.113188. Epub 2021 Mar 20.