PMID- 33799159
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1532-2777 (Electronic)
IS  - 0306-9877 (Linking)
VI  - 150
DP  - 2021 May
TI  - Laryngeal carcinoma experimental model suggests the possibility of tumor seeding 
      to gastrostomy site.
PG  - 110573
LID - S0306-9877(21)00091-8 [pii]
LID - 10.1016/j.mehy.2021.110573 [doi]
AB  - Some studies state that laryngeal squamous cell carcinoma (LSCC) is associated 
      with possible direct tumor cell seeding to percutaneous endoscopic gastrostomy 
      (PEG) site. However, there is a lack of experimental proof that LSCC tumor tissue 
      can adhere and grow in distant sites. Therefore, we aimed to investigate the 
      growth pattern of LSCC implants on chicken embryo chorioallantoic membrane (CAM) 
      and evaluate possible associations between clinical course of the disease and 
      behavior of experimentally implanted LSCC tumors. Our results show that implanted 
      LSCC tissue survives on CAMs in 95% of cases while retaining essential 
      morphologic characteristics and proliferative capacity of the original tumor. We 
      identified the increased CAM vascularization, an infiltrative growth pattern of 
      the implant and formation of distant isolated metastatic nodes on the CAMs. LSCC 
      tumors with worse differentiation degree (G2 or G3) adhered to the experimental 
      CAMs significantly better than G1. These results facilitate the understanding of 
      tumor biology and allow hypothetisezing that dissemination and direct 
      implantation of LSCC cells into the stomal wall during the pull PEG procedure 
      might be possible.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Uloza, Virgilijus
AU  - Uloza V
AD  - Department of Otorhinolaryngology, Lithuanian University of Health Sciences, 
      Eiveniu 2, Kaunas, LT 50009, Lithuania. Electronic address: 
      Virgilijus.Ulozas@lsmuni.lt.
FAU - Kuzminiene, Alina
AU  - Kuzminiene A
AD  - Department of Otorhinolaryngology, Lithuanian University of Health Sciences, 
      Eiveniu 2, Kaunas, LT 50009, Lithuania. Electronic address: 
      Alina.Kuzminiene@kaunoklinikos.lt.
FAU - Palubinskiene, Jolita
AU  - Palubinskiene J
AD  - Department of Histology and Embryology, Lithuanian University of Health Sciences, 
      A. Mickeviciaus g. 9, Kaunas, LT 44307, Lithuania. Electronic address: 
      Jolita.Palubinskiene@lsmuni.lt.
FAU - Balnyte, Ingrida
AU  - Balnyte I
AD  - Department of Histology and Embryology, Lithuanian University of Health Sciences, 
      A. Mickeviciaus g. 9, Kaunas, LT 44307, Lithuania. Electronic address: 
      Ingrida.Balnyte@lsmuni.lt.
FAU - Uloziene, Ingrida
AU  - Uloziene I
AD  - Department of Otorhinolaryngology, Lithuanian University of Health Sciences, 
      Eiveniu 2, Kaunas, LT 50009, Lithuania. Electronic address: 
      Ingrida.Uloziene@lsmuni.lt.
FAU - Valanciute, Angelija
AU  - Valanciute A
AD  - Department of Histology and Embryology, Lithuanian University of Health Sciences, 
      A. Mickeviciaus g. 9, Kaunas, LT 44307, Lithuania. Electronic address: 
      angelija.valanciute@lsmuni.lt.
LA  - eng
PT  - Journal Article
DEP - 20210323
PL  - United States
TA  - Med Hypotheses
JT  - Medical hypotheses
JID - 7505668
SB  - IM
MH  - Animals
MH  - *Carcinoma, Squamous Cell
MH  - Chick Embryo
MH  - Gastrostomy
MH  - *Laryngeal Neoplasms
MH  - Models, Theoretical
MH  - Neovascularization, Pathologic
OTO - NOTNLM
OT  - Chicken embryo CAM
OT  - Chorioallantoic membrane
OT  - Experimental models
OT  - Laryngeal carcinoma
OT  - PEG
EDAT- 2021/04/03 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/04/02 20:34
PHST- 2020/12/15 00:00 [received]
PHST- 2021/01/28 00:00 [revised]
PHST- 2021/03/13 00:00 [accepted]
PHST- 2021/04/03 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2021/04/02 20:34 [entrez]
AID - S0306-9877(21)00091-8 [pii]
AID - 10.1016/j.mehy.2021.110573 [doi]
PST - ppublish
SO  - Med Hypotheses. 2021 May;150:110573. doi: 10.1016/j.mehy.2021.110573. Epub 2021 
      Mar 23.