PMID- 33799308
OWN - NLM
STAT- MEDLINE
DCOM- 20210816
LR  - 20210816
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 13
IP  - 7
DP  - 2021 Apr 2
TI  - Longitudinal normative OCT retinal thickness data for wild-type mice, and 
      characterization of changes in the 3xTg-AD mice model of Alzheimer's disease.
PG  - 9433-9454
LID - 10.18632/aging.202916 [doi]
AB  - Mice are widely used as models for many diseases, including eye and 
      neurodegenerative diseases. However, there is a lack of normative data for 
      retinal thickness over time, especially at young ages. In this work, we present a 
      normative thickness database from one to four-months-old, for nine 
      layers/layer-aggregates, including the total retinal thickness, obtained from the 
      segmentation of spectral-domain optical coherence tomography (SD-OCT) data from 
      the C57BL6/129S mouse strain. Based on fifty-seven mice, this normative database 
      provides an opportunity to study the ageing of control mice and characterise 
      disease models' ageing, such as the triple transgenic mouse model of Alzheimer's 
      disease (3xTg-AD) used in this work. We report thickness measurements, the 
      differences in thickness per layer, demonstrate a nasal-temporal asymmetry, and 
      the variation of thickness as a function to the distance to the optic disc 
      centre. Significant differences were found between the transgenic group's 
      thickness and the normative database for the entire period covered in this study. 
      Even though it is well accepted that retinal nerve fibre layer (RNFL) thinning is 
      a hallmark of neurodegeneration, our results show a thicker RNFL-GCL 
      (RNFL-Ganglion cell layer) aggregate for the 3xTg-AD mice until four-months-old.
FAU - Ferreira, Hugo
AU  - Ferreira H
AD  - University of Coimbra, Coimbra Institute for Biomedical Imaging and Translational 
      Research (CIBIT), Institute for Nuclear Sciences Applied to Health (ICNAS), 
      Coimbra, Portugal.
FAU - Martins, Joao
AU  - Martins J
AD  - University of Coimbra, Coimbra Institute for Biomedical Imaging and Translational 
      Research (CIBIT), Institute for Nuclear Sciences Applied to Health (ICNAS), 
      Coimbra, Portugal.
AD  - University of Coimbra, Coimbra Institute for Clinical and Biomedical Research 
      (iCBR), Faculty of Medicine (FMUC), Coimbra, Portugal.
AD  - University of Coimbra, Center for Innovative Biomedicine and Biotechnology 
      (CIBB), Coimbra, Portugal.
AD  - University of Coimbra, Clinical Academic Center of Coimbra (CACC), Faculty of 
      Medicine (FMUC), Coimbra, Portugal.
FAU - Moreira, Paula I
AU  - Moreira PI
AD  - University of Coimbra, Center for Innovative Biomedicine and Biotechnology 
      (CIBB), Coimbra, Portugal.
AD  - University of Coimbra, Clinical Academic Center of Coimbra (CACC), Faculty of 
      Medicine (FMUC), Coimbra, Portugal.
AD  - University of Coimbra, Laboratory of Physiology, Faculty of Medicine (FMUC), 
      Coimbra, Portugal.
AD  - University of Coimbra, Center for Neuroscience and Cell Biology (CNC), Coimbra, 
      Portugal.
FAU - Ambrosio, Antonio Francisco
AU  - Ambrosio AF
AD  - University of Coimbra, Coimbra Institute for Clinical and Biomedical Research 
      (iCBR), Faculty of Medicine (FMUC), Coimbra, Portugal.
AD  - University of Coimbra, Center for Innovative Biomedicine and Biotechnology 
      (CIBB), Coimbra, Portugal.
AD  - University of Coimbra, Clinical Academic Center of Coimbra (CACC), Faculty of 
      Medicine (FMUC), Coimbra, Portugal.
FAU - Castelo-Branco, Miguel
AU  - Castelo-Branco M
AD  - University of Coimbra, Coimbra Institute for Biomedical Imaging and Translational 
      Research (CIBIT), Institute for Nuclear Sciences Applied to Health (ICNAS), 
      Coimbra, Portugal.
AD  - University of Coimbra, Clinical Academic Center of Coimbra (CACC), Faculty of 
      Medicine (FMUC), Coimbra, Portugal.
FAU - Serranho, Pedro
AU  - Serranho P
AD  - University of Coimbra, Coimbra Institute for Biomedical Imaging and Translational 
      Research (CIBIT), Institute for Nuclear Sciences Applied to Health (ICNAS), 
      Coimbra, Portugal.
AD  - Universidade Aberta, Department of Sciences and Technology, Lisboa, Portugal.
FAU - Bernardes, Rui
AU  - Bernardes R
AD  - University of Coimbra, Coimbra Institute for Biomedical Imaging and Translational 
      Research (CIBIT), Institute for Nuclear Sciences Applied to Health (ICNAS), 
      Coimbra, Portugal.
AD  - University of Coimbra, Clinical Academic Center of Coimbra (CACC), Faculty of 
      Medicine (FMUC), Coimbra, Portugal.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210402
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
SB  - IM
MH  - Alzheimer Disease/*diagnostic imaging/genetics
MH  - Animals
MH  - Disease Models, Animal
MH  - Mice
MH  - Mice, Transgenic
MH  - *Optic Disk
MH  - Retina/*diagnostic imaging
MH  - Tomography, Optical Coherence
PMC - PMC8064224
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - mouse model
OT  - normative data
OT  - optical coherence tomography
OT  - retinal thickness
COIS- CONFLICTS OF INTEREST: The authors declare that they have no conflicts of 
      interest.
EDAT- 2021/04/03 06:00
MHDA- 2021/08/17 06:00
PMCR- 2021/04/15
CRDT- 2021/04/02 20:38
PHST- 2020/12/06 00:00 [received]
PHST- 2021/03/23 00:00 [accepted]
PHST- 2021/04/03 06:00 [pubmed]
PHST- 2021/08/17 06:00 [medline]
PHST- 2021/04/02 20:38 [entrez]
PHST- 2021/04/15 00:00 [pmc-release]
AID - 202916 [pii]
AID - 10.18632/aging.202916 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2021 Apr 2;13(7):9433-9454. doi: 10.18632/aging.202916. Epub 
      2021 Apr 2.