PMID- 33800466
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210413
IS  - 2076-0817 (Print)
IS  - 2076-0817 (Electronic)
IS  - 2076-0817 (Linking)
VI  - 10
IP  - 3
DP  - 2021 Mar 8
TI  - A Comparative Genomic Study of Attenuated and Virulent Strains of Babesia 
      bigemina.
LID - 10.3390/pathogens10030318 [doi]
LID - 318
AB  - Cattle babesiosis is a socio-economically important tick-borne disease caused by 
      Apicomplexa protozoa of the genus Babesia that are obligate intraerythrocytic 
      parasites. The pathogenicity of Babesia parasites for cattle is determined by the 
      interaction with the host immune system and the presence of the parasite's 
      virulence genes. A Babesia bigemina strain that has been maintained under a 
      microaerophilic stationary phase in in vitro culture conditions for several years 
      in the laboratory lost virulence for the bovine host and the capacity for being 
      transmitted by the tick vector. In this study, we compared the virulome of the in 
      vitro culture attenuated Babesia bigemina strain (S) and the virulent tick 
      transmitted parental Mexican B. bigemina strain (M). Preliminary results obtained 
      by using the Basic Local Alignment Search Tool (BLAST) showed that out of 27 
      virulence genes described and analyzed in the B. bigemina virulent tick 
      transmitted strain, only five were fully identified in the attenuated laboratory 
      strain. In all cases, the identity and coverture of the identified genes of the 
      wildtype strain were higher than those of the laboratory strain. This finding is 
      putatively associated with the continuous partial loss of virulence genes in the 
      laboratory strain after several passages of the parasite population under optimal 
      in vitro growth conditions. The loss of virulence factors might be reflected in 
      the absence of symptoms of the disease in cattle inoculated with the attenuated 
      strain despite the presence of infection in the bovine host cells.
FAU - Sachman-Ruiz, Bernardo
AU  - Sachman-Ruiz B
AUID- ORCID: 0000-0001-8350-4789
AD  - CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales 
      Agricolas y Pecuarias, Jiutepec, Morelos 62550, Mexico.
FAU - Lozano, Luis
AU  - Lozano L
AD  - Centro de Ciencias Genomicas, Universidad Nacional Autonoma de Mexico, AP565-A 
      Cuernavaca, Morelos 62210, Mexico.
FAU - Lira, Jose J
AU  - Lira JJ
AD  - CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales 
      Agricolas y Pecuarias, Jiutepec, Morelos 62550, Mexico.
FAU - Martinez, Grecia
AU  - Martinez G
AUID- ORCID: 0000-0002-5795-5567
AD  - CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales 
      Agricolas y Pecuarias, Jiutepec, Morelos 62550, Mexico.
FAU - Rojas, Carmen
AU  - Rojas C
AUID- ORCID: 0000-0001-9177-5634
AD  - CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales 
      Agricolas y Pecuarias, Jiutepec, Morelos 62550, Mexico.
FAU - Alvarez, J Antonio
AU  - Alvarez JA
AD  - CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales 
      Agricolas y Pecuarias, Jiutepec, Morelos 62550, Mexico.
FAU - Figueroa, Julio V
AU  - Figueroa JV
AUID- ORCID: 0000-0003-4731-3592
AD  - CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales 
      Agricolas y Pecuarias, Jiutepec, Morelos 62550, Mexico.
LA  - eng
GR  - A1-S-43508/Consejo Nacional de Ciencia y Tecnologia/
GR  - 1672534936/INIFAP/
PT  - Journal Article
DEP - 20210308
PL  - Switzerland
TA  - Pathogens
JT  - Pathogens (Basel, Switzerland)
JID - 101596317
PMC - PMC7999288
OTO - NOTNLM
OT  - Babesia bigemina
OT  - attenuated strain
OT  - putative virulence genes
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses or interpretation of data; in 
      the writing of the manuscript or in the decision to publish the results.
EDAT- 2021/04/04 06:00
MHDA- 2021/04/04 06:01
PMCR- 2021/03/08
CRDT- 2021/04/03 01:05
PHST- 2021/01/21 00:00 [received]
PHST- 2021/02/23 00:00 [revised]
PHST- 2021/03/06 00:00 [accepted]
PHST- 2021/04/03 01:05 [entrez]
PHST- 2021/04/04 06:00 [pubmed]
PHST- 2021/04/04 06:01 [medline]
PHST- 2021/03/08 00:00 [pmc-release]
AID - pathogens10030318 [pii]
AID - pathogens-10-00318 [pii]
AID - 10.3390/pathogens10030318 [doi]
PST - epublish
SO  - Pathogens. 2021 Mar 8;10(3):318. doi: 10.3390/pathogens10030318.