PMID- 33804507
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210408
IS  - 2077-0383 (Print)
IS  - 2077-0383 (Electronic)
IS  - 2077-0383 (Linking)
VI  - 10
IP  - 5
DP  - 2021 Mar 1
TI  - Multiple Allergen Simultaneous Test-Immunoblot Assay for Immunoglobulin E 
      Detection in Patients with Isolated Allergic Conjunctivitis.
LID - 10.3390/jcm10050960 [doi]
LID - 960
AB  - We aimed to investigate the immunoglobulin E (IgE) detection rate and allergen 
      patterns in patients with isolated allergic conjunctivitis using the multiple 
      allergen simultaneous test (MAST)-immunoblot assay. A total of 120 patients with 
      allergic conjunctivitis and no associated rhinitis, asthma, or dermatitis 
      underwent the MAST-immunoblot assay to measure serum total IgE (TIgE) and serum 
      specific IgE (SIgE) against 57 allergens. Patients were classified into subgroups 
      based on the season when the eye symptoms were exacerbated, and TIgE and SIgE 
      positivity. Differences between sex and age groups were also analyzed. Of the 120 
      patients, 57.5% (69 patients) and 69.2% (83 patients) were positive for TIgE 
      (>/=100 IU/mL) and SIgE (>/=0.7 IU/mL), respectively. The allergens that most 
      frequently triggered sensitization in the study population were Dermatophagoides 
      farinae, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Alternaria, 
      and house dust. House dust mites, such as D. farinae and D. pteronyssinus, showed 
      the highest detection rates regardless of the season. Men had a higher positive 
      rate for TIgE than women, whereas a higher rate of sensitization, detected as 
      SIgE positivity, was seen in younger patients. In conclusion, MAST-immunoblot 
      assay can detect sensitizing allergens in patients with isolated allergic 
      conjunctivitis.
FAU - Han, Jung Yeob
AU  - Han JY
AUID- ORCID: 0000-0002-4092-9047
AD  - Department of Ophthalmology, Asan Medical Center, University of Ulsan College of 
      Medicine, Seoul 05505, Korea.
FAU - Lee, Hun
AU  - Lee H
AUID- ORCID: 0000-0001-7225-6580
AD  - Department of Ophthalmology, Asan Medical Center, University of Ulsan College of 
      Medicine, Seoul 05505, Korea.
FAU - Chung, Jae Lim
AU  - Chung JL
AUID- ORCID: 0000-0002-9501-9593
AD  - Eyejun Ophthalmic Clinic, Seoul 06232, Korea.
FAU - Kim, Young Jun
AU  - Kim YJ
AD  - Eyejun Ophthalmic Clinic, Seoul 06232, Korea.
FAU - Kim, Jae Yong
AU  - Kim JY
AD  - Department of Ophthalmology, Asan Medical Center, University of Ulsan College of 
      Medicine, Seoul 05505, Korea.
FAU - Tchah, Hungwon
AU  - Tchah H
AD  - Department of Ophthalmology, Asan Medical Center, University of Ulsan College of 
      Medicine, Seoul 05505, Korea.
LA  - eng
GR  - P0014063/Ministry of Trade, Industry and Energy/
GR  - 2020M3E5D8104338, 2020M3E5D8106706/Ministry of Science and ICT, South Korea/
PT  - Journal Article
DEP - 20210301
PL  - Switzerland
TA  - J Clin Med
JT  - Journal of clinical medicine
JID - 101606588
PMC - PMC7957652
OTO - NOTNLM
OT  - allergic conjunctivitis
OT  - multiple allergen simultaneous test-immunoblot assay
OT  - serum immunoglobulin E
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2021/04/04 06:00
MHDA- 2021/04/04 06:01
PMCR- 2021/03/01
CRDT- 2021/04/03 01:17
PHST- 2021/01/16 00:00 [received]
PHST- 2021/02/15 00:00 [revised]
PHST- 2021/02/19 00:00 [accepted]
PHST- 2021/04/03 01:17 [entrez]
PHST- 2021/04/04 06:00 [pubmed]
PHST- 2021/04/04 06:01 [medline]
PHST- 2021/03/01 00:00 [pmc-release]
AID - jcm10050960 [pii]
AID - jcm-10-00960 [pii]
AID - 10.3390/jcm10050960 [doi]
PST - epublish
SO  - J Clin Med. 2021 Mar 1;10(5):960. doi: 10.3390/jcm10050960.