PMID- 33806267
OWN - NLM
STAT- MEDLINE
DCOM- 20210824
LR  - 20210824
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 13
IP  - 3
DP  - 2021 Mar 4
TI  - Characterization and Vector Competence Studies of Chikungunya Virus Lacking 
      Repetitive Motifs in the 3' Untranslated Region of the Genome.
LID - 10.3390/v13030403 [doi]
LID - 403
AB  - Using reverse genetics, we analyzed a chikungunya virus (CHIKV) isolate of the 
      Indian Ocean lineage lacking direct repeat (DR) elements in the 3' untranslated 
      region, namely DR1a and DR2a. While this deletion mutant CHIKV-∆DR exhibited 
      growth characteristics comparable to the wild-type virus in Baby Hamster Kidney 
      cells, replication of the mutant was reduced in Aedes albopictus C6/36 and Ae. 
      aegypti Aag2 cells. Using oral and intrathoracic infection of mosquitoes, viral 
      infectivity, dissemination, and transmission of CHIKV-∆DR could be shown for the 
      well-known CHIKV vectors Ae. aegypti and Ae. albopictus. Oral infection of Ae. 
      vexans and Culex pipiens mosquitoes with mutant or wild-type CHIKV showed very 
      limited infectivity. Dissemination, transmission, and transmission efficiencies 
      as determined via viral RNA in the saliva were slightly higher in Ae. vexans for 
      the wild-type virus than for CHIKV-∆DR. However, both Ae. vexans and Cx. pipiens 
      allowed efficient viral replication after intrathoracic injection confirming that 
      the midgut barrier is an important determinant for the compromised infectivity 
      after oral infection. Transmission efficiencies were neither significantly 
      different between Ae. vexans and Cx. pipiens nor between wild-type and CHIKV-∆DR. 
      With a combined transmission efficiency of 6%, both Ae. vexans and Cx. pipiens 
      might serve as potential vectors in temperate regions.
FAU - Karliuk, Yauhen
AU  - Karliuk Y
AD  - Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary 
      Medicine, University of Leipzig, 04103 Leipzig, Germany.
FAU - Vom Hemdt, Anja
AU  - Vom Hemdt A
AUID- ORCID: 0000-0003-0814-5979
AD  - Institute of Virology, Medical Faculty, University of Bonn, 53127 Bonn, Germany.
FAU - Wieseler, Janett
AU  - Wieseler J
AD  - Institute of Virology, Medical Faculty, University of Bonn, 53127 Bonn, Germany.
FAU - Pfeffer, Martin
AU  - Pfeffer M
AUID- ORCID: 0000-0002-2457-8224
AD  - Institute of Animal Hygiene and Veterinary Public Health, Faculty of Veterinary 
      Medicine, University of Leipzig, 04103 Leipzig, Germany.
FAU - Kummerer, Beate M
AU  - Kummerer BM
AUID- ORCID: 0000-0002-9011-2764
AD  - Institute of Virology, Medical Faculty, University of Bonn, 53127 Bonn, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210304
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (3' Untranslated Regions)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Aedes/*virology
MH  - Animals
MH  - Chikungunya Fever/*virology
MH  - Chikungunya virus/*genetics
MH  - Chlorocebus aethiops
MH  - Cricetinae
MH  - Culex/*virology
MH  - Genes, Viral
MH  - Mosquito Vectors/*virology
MH  - Vero Cells
PMC - PMC7999577
OTO - NOTNLM
OT  - 3' untranslated region
OT  - Aedes vexans
OT  - Culex pipiens
OT  - chikungunya virus
OT  - direct repeats
OT  - vector competence
COIS- The authors declare no conflict of interest.
EDAT- 2021/04/04 06:00
MHDA- 2021/08/25 06:00
PMCR- 2021/03/04
CRDT- 2021/04/03 01:23
PHST- 2021/01/26 00:00 [received]
PHST- 2021/02/26 00:00 [revised]
PHST- 2021/02/27 00:00 [accepted]
PHST- 2021/04/03 01:23 [entrez]
PHST- 2021/04/04 06:00 [pubmed]
PHST- 2021/08/25 06:00 [medline]
PHST- 2021/03/04 00:00 [pmc-release]
AID - v13030403 [pii]
AID - viruses-13-00403 [pii]
AID - 10.3390/v13030403 [doi]
PST - epublish
SO  - Viruses. 2021 Mar 4;13(3):403. doi: 10.3390/v13030403.