PMID- 33806347
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210413
IS  - 2304-8158 (Print)
IS  - 2304-8158 (Electronic)
IS  - 2304-8158 (Linking)
VI  - 10
IP  - 3
DP  - 2021 Mar 4
TI  - Dietary Grape Seed Meal Bioactive Compounds Alleviate Epithelial Dysfunctions and 
      Attenuates Inflammation in Colon of DSS-Treated Piglets.
LID - 10.3390/foods10030530 [doi]
LID - 530
AB  - Inflammatory Bowel Diseases (IBD) are chronic inflammations associated with 
      progressive degradation of intestinal epithelium and impairment of the local 
      innate immune response. Restoring of epithelial integrity and of the mucosal 
      barrier function, together with modulation of inflammatory and innate immune 
      markers, represent targets for alternative strategies in IBD. The aim of our 
      study was to evaluate the effects of a diet including 8% grape seed meal (GSM), 
      rich in bioactive compounds (polyphenols, polyunsaturated fatty acids (PUFAs), 
      fiber) on the markers of colonic epithelial integrity, mucosal barrier function, 
      pro-inflammatory, and innate immunity in DSS-treated piglets used as animal 
      models of intestinal inflammation. Our results have demonstrated the beneficial 
      effects of bioactive compounds from dietary GSM, exerted at three complementary 
      levels: (a) restoration of the epithelial integrity and mucosal barrier 
      reinforcement by modulation of claudins, Occludin (OCCL) and Zonula-1 (ZO-1) 
      tight junction genes and proteins, myosin IXB (MYO9B) and protein tyrosine 
      phosphatase (PTPN) tight junction regulators and mucin-2 (MUC2) gene; (b) 
      reduction of pro-inflammatory MMP-2 (matrix metalloproteinase-2) and MMP-9 
      (matrix metalloproteinase-9) genes and activities; and (c) suppression of the 
      innate immune TLR-2 (Toll-like receptor-2) and TLR-4 (Toll-like receptor-4) genes 
      and attenuation of the expression of MyD88 (Myeloid Differentiation Primary 
      Response 88)/MD-2 (Myeloid differentiation factor-2) signaling molecules. These 
      beneficial effects of GSM could further attenuate the transition of chronic 
      colitis to carcinogenesis, by modulating the in-depth signaling mediators 
      belonging to the Wnt pathway.
FAU - Pistol, Gina Cecilia
AU  - Pistol GC
AUID- ORCID: 0000-0003-1139-604X
AD  - Laboratory of Animal Biology, INCDBNA-IBNA, National Institute of Research and 
      Development for Biology and Animal Nutrition, 077015 Balotesti, Romania.
FAU - Bulgaru, Cristina Valeria
AU  - Bulgaru CV
AD  - Laboratory of Animal Biology, INCDBNA-IBNA, National Institute of Research and 
      Development for Biology and Animal Nutrition, 077015 Balotesti, Romania.
FAU - Marin, Daniela Eliza
AU  - Marin DE
AD  - Laboratory of Animal Biology, INCDBNA-IBNA, National Institute of Research and 
      Development for Biology and Animal Nutrition, 077015 Balotesti, Romania.
FAU - Oancea, Alexandra Gabriela
AU  - Oancea AG
AD  - Laboratory of Chemistry and Nutrition Physiology, INCDBNA-IBNA, National 
      Institute of Research and Development for Biology and Animal Nutrition, 077015 
      Balotesti, Romania.
FAU - Taranu, Ionelia
AU  - Taranu I
AUID- ORCID: 0000-0003-0727-5827
AD  - Laboratory of Animal Biology, INCDBNA-IBNA, National Institute of Research and 
      Development for Biology and Animal Nutrition, 077015 Balotesti, Romania.
LA  - eng
GR  - PN-II-RU-TE-2014-4-1287/Ministerul Cercetarii si Inovarii/
PT  - Journal Article
DEP - 20210304
PL  - Switzerland
TA  - Foods
JT  - Foods (Basel, Switzerland)
JID - 101670569
PMC - PMC7999447
OTO - NOTNLM
OT  - animal model
OT  - epithelium
OT  - grape
OT  - inflammation
OT  - inflammatory bowel disease
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2021/04/04 06:00
MHDA- 2021/04/04 06:01
PMCR- 2021/03/04
CRDT- 2021/04/03 01:23
PHST- 2021/01/12 00:00 [received]
PHST- 2021/02/25 00:00 [revised]
PHST- 2021/02/27 00:00 [accepted]
PHST- 2021/04/03 01:23 [entrez]
PHST- 2021/04/04 06:00 [pubmed]
PHST- 2021/04/04 06:01 [medline]
PHST- 2021/03/04 00:00 [pmc-release]
AID - foods10030530 [pii]
AID - foods-10-00530 [pii]
AID - 10.3390/foods10030530 [doi]
PST - epublish
SO  - Foods. 2021 Mar 4;10(3):530. doi: 10.3390/foods10030530.