PMID- 33810054
OWN - NLM
STAT- MEDLINE
DCOM- 20210928
LR  - 20210928
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 11
IP  - 3
DP  - 2021 Mar 22
TI  - Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats.
LID - 10.3390/biom11030472 [doi]
LID - 472
AB  - Endometrial receptivity represents one of the leading factors affecting the 
      successful implantation of embryos during early pregnancy. However, the mechanism 
      of microRNAs (miRNAs) to establish goat endometrial receptivity remains unclear. 
      This study was intended to identify potential miRNAs and regulatory mechanisms 
      associated with establishing endometrial receptivity through integrating 
      bioinformatics analysis and experimental verification. MiRNA expression profiles 
      were obtained by high-throughput sequencing, resulting in the detection of 33 
      differentially expressed miRNAs (DEMs), followed by their validation through 
      quantitative RT-PCR. Furthermore, 10 potential transcription factors (TFs) and 
      1316 target genes of these DEMs were obtained, and the TF-miRNA and miRNA-mRNA 
      interaction networks were constructed. Gene Ontology (GO) and Kyoto Encyclopedia 
      of Genes and Genomes (KEGG) analyses indicated that these miRNAs were 
      significantly linked to establishing endometrial receptivity. Moreover, the 
      fluorescence in situ hybridization (FISH) analysis, dual-luciferase report assay, 
      and immunohistochemistry (IHC) analysis corroborated that chi-miR-483 could 
      directly bind to deltex E3 ubiquitin ligase 3L (DTX3L) to reduce its expression 
      level. In conclusion, our findings contribute to a better understanding of 
      molecular mechanisms regulating the endometrial receptivity of goats, and they 
      provide a reference for improving embryo implantation efficiency.
FAU - Zang, Xupeng
AU  - Zang X
AD  - College of Animal Science, South China Agricultural University, Guangzhou 510642, 
      China.
AD  - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China.
FAU - Zhou, Chen
AU  - Zhou C
AD  - College of Animal Science, South China Agricultural University, Guangzhou 510642, 
      China.
AD  - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China.
FAU - Wang, Wenjing
AU  - Wang W
AD  - College of Animal Science, South China Agricultural University, Guangzhou 510642, 
      China.
AD  - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China.
FAU - Gan, Jianyu
AU  - Gan J
AD  - College of Animal Science, South China Agricultural University, Guangzhou 510642, 
      China.
AD  - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China.
FAU - Li, Yaokun
AU  - Li Y
AUID- ORCID: 0000-0002-9211-0244
AD  - College of Animal Science, South China Agricultural University, Guangzhou 510642, 
      China.
AD  - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China.
FAU - Liu, Dewu
AU  - Liu D
AD  - College of Animal Science, South China Agricultural University, Guangzhou 510642, 
      China.
AD  - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China.
FAU - Liu, Guangbin
AU  - Liu G
AD  - College of Animal Science, South China Agricultural University, Guangzhou 510642, 
      China.
AD  - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China.
FAU - Hong, Linjun
AU  - Hong L
AUID- ORCID: 0000-0002-6554-7918
AD  - College of Animal Science, South China Agricultural University, Guangzhou 510642, 
      China.
AD  - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China.
LA  - eng
GR  - 2018-143/Guangdong Provincial Promotion Project on Preservation and Utilization 
      of Local Breed of Live-stock and Poultry/
GR  - 2019KJ127/Modern Agricultural Industry Technology System of Guangdong Province/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210322
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Embryo Implantation/physiology
MH  - Endometrium/*metabolism
MH  - Female
MH  - Gene Ontology
MH  - Goats
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - MicroRNAs/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC8004627
OTO - NOTNLM
OT  - DTX3L
OT  - chi-miR-483
OT  - endometrium receptivity
OT  - goat
OT  - high-throughput sequencing
OT  - implantation
COIS- The authors declare no conflict of interest.
EDAT- 2021/04/04 06:00
MHDA- 2021/09/29 06:00
PMCR- 2021/03/22
CRDT- 2021/04/03 01:35
PHST- 2021/01/25 00:00 [received]
PHST- 2021/03/08 00:00 [revised]
PHST- 2021/03/18 00:00 [accepted]
PHST- 2021/04/03 01:35 [entrez]
PHST- 2021/04/04 06:00 [pubmed]
PHST- 2021/09/29 06:00 [medline]
PHST- 2021/03/22 00:00 [pmc-release]
AID - biom11030472 [pii]
AID - biomolecules-11-00472 [pii]
AID - 10.3390/biom11030472 [doi]
PST - epublish
SO  - Biomolecules. 2021 Mar 22;11(3):472. doi: 10.3390/biom11030472.