PMID- 33812640 OWN - NLM STAT- MEDLINE DCOM- 20210514 LR - 20210514 IS - 1873-0191 (Electronic) IS - 0928-4931 (Linking) VI - 123 DP - 2021 Apr TI - One-minute and green synthesis of magnetic iron oxide nanoparticles assisted by design of experiments and high energy ultrasound: Application to biosensing and immunoprecipitation. PG - 112023 LID - S0928-4931(21)00162-4 [pii] LID - 10.1016/j.msec.2021.112023 [doi] AB - The present study is focused on the ultrafast and green synthesis, via the co-precipitation method, of magnetic nanoparticles (MNPs) based on iron oxides using design of experiments (DOE) and high energy sonochemical approach, considering two main factors: amplitude (energy) of the ultrasound probe and sonication time. The combination of these techniques allowed the development of a novel one-minute green synthesis, which drastically reduced the amount of consumed energy, solvents, reagents, time and produced residues. This green sonochemical synthesis permitted to obtain mean particle sizes of 11 +/- 2 nm under the optimized conditions of amplitude = 40% (2826 J) and time = 1 min. Their composition, structure, size, morphology and magnetic properties were assessed through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning and transmission electron microscopy (SEM & TEM), and vibrating sample magnetometry (VSM). The characterization results indicate the proper formation of MNPs, and the correct functionalization of MNPs with different coating agents. The functionalized MNPs were used as: i) biosensor, which could detect mercury in water in the range of 0.030-0.060 ppm, and ii) support onto which polyclonal antibodies were anchored and successfully bound to an osteosarcoma cell line expressing the target protein (TRIB2-GFP), as part of an immunoprecipitation assay. CI - Copyright (c) 2021 Elsevier B.V. All rights reserved. FAU - Perez-Beltran, Christian Hazael AU - Perez-Beltran CH AD - Institute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Poligono del Rio San Pedro, S/N, 11510 Puerto Real, Cadiz, Spain; Faculty of Science and Technology, Universidade do Algarve, Campus Gambelas, Faro 8005-139, Portugal. FAU - Garcia-Guzman, Juan Jose AU - Garcia-Guzman JJ AD - Institute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Poligono del Rio San Pedro, S/N, 11510 Puerto Real, Cadiz, Spain. FAU - Ferreira, Bibiana AU - Ferreira B AD - Centre for Biomedical Research (CBMR), Universidade do Algarve, Campus Gambelas, Faro 8005-139, Portugal; Algarve Biomedical Center (ABC), University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal; Regenerative Medicine Program, Department of Biomedical Sciences and Medicine, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal. FAU - Estevez-Hernandez, Osvaldo AU - Estevez-Hernandez O AD - Instituto de Ciencia y Tecnologia de Materiales (IMRE), Universidad de La Habana, Zapata y G, Vedado 10400, La Habana, Cuba. FAU - Lopez-Iglesias, David AU - Lopez-Iglesias D AD - Institute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Poligono del Rio San Pedro, S/N, 11510 Puerto Real, Cadiz, Spain. FAU - Cubillana-Aguilera, Laura AU - Cubillana-Aguilera L AD - Institute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Poligono del Rio San Pedro, S/N, 11510 Puerto Real, Cadiz, Spain. FAU - Link, Wolfgang AU - Link W AD - Instituto de Investigaciones Biomedicas "Alberto Sols" (CSIC-UAM), Arturo Duperier 4, 28029 Madrid, Spain. FAU - Stanica, N AU - Stanica N AD - Institute of Physical Chemistry 'Ilie Murgulescu' of the Romanian Academy, 202 Splaiul Independentei, 060021 Bucharest, Romania. FAU - Rosa da Costa, Ana Maria AU - Rosa da Costa AM AD - Faculty of Science and Technology, Universidade do Algarve, Campus Gambelas, Faro 8005-139, Portugal; Algarve Chemistry Research Centre (CIQA), Universidade do Algarve, Campus Gambelas, Faro 8005-139, Portugal. Electronic address: amcosta@ualg.pt. FAU - Palacios-Santander, Jose Maria AU - Palacios-Santander JM AD - Institute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Poligono del Rio San Pedro, S/N, 11510 Puerto Real, Cadiz, Spain. Electronic address: josem.palacios@uca.es. LA - eng PT - Journal Article DEP - 20210310 PL - Netherlands TA - Mater Sci Eng C Mater Biol Appl JT - Materials science & engineering. C, Materials for biological applications JID - 101484109 RN - 0 (Magnetite Nanoparticles) SB - IM MH - Immunoprecipitation MH - Magnetic Iron Oxide Nanoparticles MH - Magnetic Phenomena MH - Magnetics MH - *Magnetite Nanoparticles MH - Spectroscopy, Fourier Transform Infrared OTO - NOTNLM OT - 1 min-synthesis OT - Biosensing OT - Design of experiments OT - High-energy ultrasound OT - Immunoprecipitation OT - Iron oxides OT - Magnetic nanoparticles EDAT- 2021/04/05 06:00 MHDA- 2021/05/15 06:00 CRDT- 2021/04/04 20:27 PHST- 2020/11/20 00:00 [received] PHST- 2021/02/08 00:00 [revised] PHST- 2021/03/02 00:00 [accepted] PHST- 2021/04/04 20:27 [entrez] PHST- 2021/04/05 06:00 [pubmed] PHST- 2021/05/15 06:00 [medline] AID - S0928-4931(21)00162-4 [pii] AID - 10.1016/j.msec.2021.112023 [doi] PST - ppublish SO - Mater Sci Eng C Mater Biol Appl. 2021 Apr;123:112023. doi: 10.1016/j.msec.2021.112023. Epub 2021 Mar 10.