PMID- 33813273
OWN - NLM
STAT- MEDLINE
DCOM- 20210630
LR  - 20210630
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 555
DP  - 2021 May 28
TI  - Requirement for expression of WW domain containing transcription regulator 1 in 
      bovine trophectoderm development.
PG  - 140-146
LID - S0006-291X(21)00510-6 [pii]
LID - 10.1016/j.bbrc.2021.03.112 [doi]
AB  - WW domain-containing transcription regulator 1 (WWTR1) is one of the primary 
      effectors in the Hippo pathway, which plays essential roles in cell 
      differentiation into trophectoderm (TE) and inner cell mass cell lineages at the 
      blastocyst stage. However, little is known about the roles of WWTR1 in 
      preimplantation development. The present study aimed to explore the significance 
      of WWTR1 expression in preimplantation development using an mRNA knockdown (KD) 
      system in bovine embryos. We first quantitated WWTR1 expression at protein and 
      mRNA levels from fertilization to blastocyst stage. WWTR1 proteins gradually 
      shifted from extranuclear localization during the 16-cell stage to nuclear 
      localization by morula stage. WWTR1 mRNA expression was also transiently 
      upregulated at the 16-cell stage. WWTR1 KD efficiently repressed WWTR1 expression 
      at protein and mRNA levels. The WWTR1 KD embryos developed to the blastocyst 
      stage at rates equivalent to those of controls, but TE cell numbers were 
      significantly decreased. Representative TE-expressed genes, including CDX2 and 
      IFNT were also significantly decreased in WWTR1 KD blastocysts. These results 
      provide the first demonstration that WWTR1 expression is responsible for normal 
      TE cell development in preimplantation embryos.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Saito, Shun
AU  - Saito S
AD  - Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, 
      Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan.
FAU - Yamamura, Shota
AU  - Yamamura S
AD  - Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, 
      Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan.
FAU - Kohri, Nanami
AU  - Kohri N
AD  - Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, 
      Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan.
FAU - Bai, Hanako
AU  - Bai H
AD  - Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, 
      Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan.
FAU - Takahashi, Masashi
AU  - Takahashi M
AD  - Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, 
      Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan.
FAU - Kawahara, Manabu
AU  - Kawahara M
AD  - Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, 
      Hokkaido University, Kita-ku, Kita 9, Nishi 9, Sapporo, 060-8589, Japan. 
      Electronic address: k-hara@anim.agr.hokudai.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210401
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*genetics/metabolism
MH  - Animals
MH  - Blastocyst/cytology/*physiology
MH  - Cattle
MH  - Cell Differentiation/genetics
MH  - Embryonic Development/*physiology
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Oocytes/physiology
MH  - RNA, Messenger/genetics
MH  - RNA, Small Interfering
MH  - Trophoblasts/physiology
MH  - WW Domains
OTO - NOTNLM
OT  - Blastocyst
OT  - Cattle
OT  - Differentiation
OT  - Embryo
OT  - Hippo pathway
OT  - Trophectoderm
COIS- Declaration of competing interest There are no conflicts of interest to declare.
EDAT- 2021/04/05 06:00
MHDA- 2021/07/01 06:00
CRDT- 2021/04/04 20:53
PHST- 2021/03/08 00:00 [received]
PHST- 2021/03/20 00:00 [accepted]
PHST- 2021/04/05 06:00 [pubmed]
PHST- 2021/07/01 06:00 [medline]
PHST- 2021/04/04 20:53 [entrez]
AID - S0006-291X(21)00510-6 [pii]
AID - 10.1016/j.bbrc.2021.03.112 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2021 May 28;555:140-146. doi: 
      10.1016/j.bbrc.2021.03.112. Epub 2021 Apr 1.