PMID- 33814154
OWN - NLM
STAT- MEDLINE
DCOM- 20210622
LR  - 20240102
IS  - 1525-3198 (Electronic)
IS  - 0022-0302 (Linking)
VI  - 104
IP  - 7
DP  - 2021 Jul
TI  - Role of long polar fimbriae type 1 and 2 in pathogenesis of mammary pathogenic 
      Escherichia coli.
PG  - 8243-8255
LID - S0022-0302(21)00488-4 [pii]
LID - 10.3168/jds.2021-20122 [doi]
AB  - Escherichia coli is a leading cause of bovine mastitis worldwide. The bacteria 
      can rapidly grow in milk and elicit a strong lipopolysaccharide (LPS)/toll-like 
      receptor-4 (TLR4)-dependent inflammatory response. Recently, the long polar 
      fimbriae (LPF) were identified as a promising virulence factor candidate widely 
      distributed in mammary pathogenic E. coli (MPEC) strains. Mammary pathogenic E. 
      coli possess 2 lpf loci encoding LPF1 and LPF2, respectively. By deleting the 
      major fimbrial subunit gene, lpfA, we found that both LPF1 and LPF2 contribute to 
      MPEC adhesion, invasion, and biofilm formation in vitro. The lpf1A and lpf2A 
      mutants showed reduced cytotoxicity in our in vitro cell infection model. 
      Furthermore, we observed that LPF2 induced a mild TLR4-independent 
      proinflammatory response. The median lethal dose (LD(50)) of both ∆lpf2A and 
      ∆lpf1A∆lpf2A mutants to BALB/c mice increased by 0.38 and 0.15 logs, 
      respectively, whereas that of wild-type strain MPJS13 was 8.69 logs. In contrast, 
      LPF1 deficiency significantly enhanced the LPS/TLR4-mediated inflammatory 
      response in mammary epithelial cells, and the LD(50) of the mutant decreased to 
      8.18 logs. In conclusion, our data suggested that LPF are important in MPEC 
      colonization of mammary cells and may provide a benefit to bacterial 
      intracellular survival that induces persistent bovine mastitis.
CI  - Copyright (c) 2021 American Dairy Science Association. Published by Elsevier Inc. 
      All rights reserved.
FAU - Zhou, Mingxu
AU  - Zhou M
AD  - Institute of Veterinary Immunology & Engineering, National Research Center of 
      Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of 
      Agricultural Sciences, Nanjing 210014, China; Jiangsu Key Laboratory for Food 
      Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and 
      Technology, Nanjing 210014, China.
FAU - Yang, Yang
AU  - Yang Y
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety of 
      Ministry of Education of China; College of Veterinary Medicine, Yangzhou 
      University, Yangzhou 225009, China.
FAU - Wu, Miaomiao
AU  - Wu M
AD  - College of Animal Science and Technology, Hunan Co-Innovation Center of Animal 
      Production Safety, Hunan Agricultural University, Changsha 410128, China.
FAU - Ma, Fang
AU  - Ma F
AD  - Institute of Veterinary Immunology & Engineering, National Research Center of 
      Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of 
      Agricultural Sciences, Nanjing 210014, China.
FAU - Xu, Yue
AU  - Xu Y
AD  - Institute of Veterinary Immunology & Engineering, National Research Center of 
      Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of 
      Agricultural Sciences, Nanjing 210014, China.
FAU - Deng, Bihua
AU  - Deng B
AD  - Institute of Veterinary Immunology & Engineering, National Research Center of 
      Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of 
      Agricultural Sciences, Nanjing 210014, China.
FAU - Zhang, Jinqiu
AU  - Zhang J
AD  - Institute of Veterinary Immunology & Engineering, National Research Center of 
      Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of 
      Agricultural Sciences, Nanjing 210014, China.
FAU - Zhu, Guoqiang
AU  - Zhu G
AD  - Joint International Research Laboratory of Agriculture and Agri-Product Safety of 
      Ministry of Education of China; College of Veterinary Medicine, Yangzhou 
      University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention 
      and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 
      225009, China. Electronic address: yzgqzhu@yzu.edu.cn.
FAU - Lu, Yu
AU  - Lu Y
AD  - Institute of Veterinary Immunology & Engineering, National Research Center of 
      Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of 
      Agricultural Sciences, Nanjing 210014, China; School of Pharmacy, Jiangsu 
      University, Zhenjiang 212013, China. Electronic address: luyu@jaas.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20210402
PL  - United States
TA  - J Dairy Sci
JT  - Journal of dairy science
JID - 2985126R
RN  - 0 (Escherichia coli Proteins)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Cattle Diseases
MH  - Escherichia coli
MH  - *Escherichia coli Infections/veterinary
MH  - *Escherichia coli Proteins
MH  - Female
MH  - Fimbriae, Bacterial
MH  - Mice
MH  - Mice, Inbred BALB C
OTO - NOTNLM
OT  - Escherichia coli
OT  - inflammatory response
OT  - long polar fimbriae
OT  - mastitis
OT  - pathogenesis
EDAT- 2021/04/06 06:00
MHDA- 2021/06/23 06:00
CRDT- 2021/04/05 05:37
PHST- 2021/01/04 00:00 [received]
PHST- 2021/02/22 00:00 [accepted]
PHST- 2021/04/06 06:00 [pubmed]
PHST- 2021/06/23 06:00 [medline]
PHST- 2021/04/05 05:37 [entrez]
AID - S0022-0302(21)00488-4 [pii]
AID - 10.3168/jds.2021-20122 [doi]
PST - ppublish
SO  - J Dairy Sci. 2021 Jul;104(7):8243-8255. doi: 10.3168/jds.2021-20122. Epub 2021 
      Apr 2.